| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,763 | G→A | 79.4% | intergenic (‑134/+162) | yqeF ← / ← lysS | predicted acyltransferase/lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,763 | 0 | G | A | 79.4% | 196.8 / 46.4 | 102 | intergenic (‑134/+162) | yqeF/lysS | predicted acyltransferase/lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (9/12); new base A (30/51); total (39/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.74e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
TAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGT > minE/1932695‑1932830 | tAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAagg > 1:2833520/1‑71 (MQ=255) aaaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAagg < 1:2542123/70‑1 (MQ=255) aaaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGt > 1:5480343/1‑71 (MQ=255) aaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAagg < 1:1475786/69‑1 (MQ=255) aaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGt < 1:466989/70‑1 (MQ=255) aaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGtt < 1:484226/71‑1 (MQ=255) aaaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGt < 1:2724405/70‑1 (MQ=255) aaaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGttt < 1:2087578/71‑1 (MQ=255) aaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTg < 1:4568146/71‑1 (MQ=255) aaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTg < 1:2322765/71‑1 (MQ=255) aaaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTg > 1:4992381/1‑71 (MQ=255) aaaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc < 1:4526543/71‑1 (MQ=255) aaGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc < 1:1993085/70‑1 (MQ=255) gTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAAc < 1:1081794/71‑1 (MQ=255) gTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAAc < 1:274317/71‑1 (MQ=255) gATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAagg > 1:205636/1‑61 (MQ=255) tttttATCACATAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt < 1:3988933/71‑1 (MQ=255) tttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt < 1:2596593/71‑1 (MQ=255) tttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt < 1:189235/71‑1 (MQ=255) ttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGttt > 1:945466/1‑61 (MQ=255) ttttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCaa > 1:3210075/1‑65 (MQ=255) tttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTaa > 1:4467836/1‑71 (MQ=255) tttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc > 1:4635771/1‑62 (MQ=255) ttATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAAc > 1:4384860/1‑71 (MQ=255) tCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAAcc < 1:224332/69‑1 (MQ=255) acaAAGGAAATATGCCTGAGCACCAGTCAGAGACATAACTGGCACGTAAGGtt > 1:5559763/1‑53 (MQ=255) aaaGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCa < 1:774399/55‑1 (MQ=255) aaaGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCa < 1:1151202/55‑1 (MQ=255) aaGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAAccc < 1:1527812/65‑1 (MQ=255) aaGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAAt < 1:7641/71‑1 (MQ=255) ggAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATag < 1:1744577/71‑1 (MQ=255) gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCa > 1:2459952/1‑55 (MQ=255) gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCa > 1:2816374/1‑55 (MQ=255) gAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGAAAcc < 1:5080674/54‑1 (MQ=255) aaaTATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATagag > 1:206936/1‑71 (MQ=255) atatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAggg < 1:5524019/71‑1 (MQ=255) tatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGc < 1:2963592/46‑1 (MQ=255) tatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt < 1:5629948/53‑1 (MQ=255) tatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACt < 1:5674813/53‑1 (MQ=255) tatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAgggg < 1:4119660/71‑1 (MQ=255) tatGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAgggg < 1:5541179/71‑1 (MQ=255) tGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTa < 1:3654125/71‑1 (MQ=255) tGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTa < 1:2967586/71‑1 (MQ=255) tGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTa < 1:4917049/71‑1 (MQ=255) ccTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa < 1:4189176/71‑1 (MQ=255) cTGAGCAGCAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa > 1:3184108/1‑70 (MQ=255) tGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa > 1:2712129/1‑69 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGAt < 1:5269958/68‑1 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAgg > 1:1890582/1‑71 (MQ=255) agcagcAGTCAGAGACATAACTGGCACGTAAGGTTTGCAACAACTAACCCACCAATAGAGGGGTAGATAg > 1:2326976/1‑70 (MQ=255) cagcagTCAGAGACATAACTGGCACGTAAGGTTTGCAA‑CACTAACcca > 1:1053511/1‑48 (MQ=38) cagTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGtt < 1:559915/71‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:845870/71‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:3469119/71‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:3856124/71‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:2676576/71‑1 (MQ=255) agTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:3033175/71‑1 (MQ=255) gTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTaa < 1:2553984/71‑1 (MQ=255) gTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGa < 1:2054094/60‑1 (MQ=255) gTCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:5497369/70‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACTACTAACCCACCAATa < 1:807888/49‑1 (MQ=39) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACccac < 1:1758029/44‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACccac < 1:287773/44‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:4457034/49‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:2067098/49‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:1621634/49‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:5649948/49‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:1338540/49‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa < 1:2680932/49‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg > 1:4861620/1‑54 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg > 1:243982/1‑54 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg > 1:1958093/1‑54 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg > 1:4777539/1‑54 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg > 1:813669/1‑54 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTa < 1:667527/69‑1 (MQ=255) tCAGAGACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAAt > 1:5142764/1‑71 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACccacca > 1:2075483/1‑44 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACccacca > 1:507672/1‑44 (MQ=255) agagACATAACTGGCACGTAGGGTTTGCAACCACTAACccacca > 1:4467516/1‑44 (MQ=255) agagACATAACTGGCACGTAAGGTTTGCAACCACTAACccacca > 1:3871246/1‑44 (MQ=255) agagACATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTaa < 1:1465022/68‑1 (MQ=255) gagaCATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAgggg < 1:3092639/52‑1 (MQ=255) agaCATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTcc < 1:1611959/71‑1 (MQ=255) cATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCa < 1:4975227/70‑1 (MQ=255) cATAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCAt < 1:5055109/71‑1 (MQ=255) aTAACTGGCACGTAGGGTTTGCAACCACTAACcca > 1:817344/1‑35 (MQ=255) aTAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTcc < 1:3133098/67‑1 (MQ=255) aTAACTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTcc < 1:1765872/67‑1 (MQ=255) tAACTGGCACGTAGGGTTTGCAACCATTAACCCACCAATAGAGGGGTAGATAGGGCGt < 1:4131597/58‑1 (MQ=255) tAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGt > 1:5208252/1‑58 (MQ=255) aaCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACt > 1:568684/1‑71 (MQ=255) aCTGGCACGTAAGGTTTGCAACCACTAACCCACCAATa > 1:2993213/1‑38 (MQ=255) tGGCACGTAAGGTTTGCAACCACTAACCCACCAATAGAggg < 1:1114710/41‑1 (MQ=255) ggCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAggg > 1:2186694/1‑50 (MQ=255) ggCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAggg > 1:1548937/1‑50 (MQ=255) gCACGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAAcc < 1:5537407/71‑1 (MQ=255) aCGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTg < 1:4927887/71‑1 (MQ=255) cGTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTgg > 1:3652166/1‑71 (MQ=255) gTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGt > 1:1681499/1‑71 (MQ=255) gTAAGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGt > 1:4150523/1‑71 (MQ=255) | TAAAAAAAGTGATTTTTATCACAAAGGAAATATGCCTGAGCAGCAGTCAGAGACATAACTGGCACGTAGGGTTTGCAACCACTAACCCACCAATAGAGGGGTAGATAGGGCGTTAATCTCCCATACTTAACCTGGT > minE/1932695‑1932830 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |