| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,970 | G→A | 25.4% | T491T (ACC→ACT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,970 | 0 | G | A | 25.4% | 212.4 / 72.6 | 126 | T491T (ACC→ACT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (59/35); new base A (21/11); total (80/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.34e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 133 total aligned reads displayed.
CGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGT > minE/1932900‑1933036 | cGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:488848/1‑71 (MQ=255) ggggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg < 1:3836129/71‑1 (MQ=255) gggTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg > 1:602358/1‑70 (MQ=255) gTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt < 1:4277311/71‑1 (MQ=255) gTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGAGGATGGTAt > 1:4475544/1‑71 (MQ=255) ttGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATg > 1:5326697/1‑71 (MQ=255) gggCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTTTGGCt < 1:4852350/68‑1 (MQ=255) aGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGt < 1:2807755/71‑1 (MQ=255) gCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:3595818/1‑71 (MQ=255) cATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATgg < 1:2753239/60‑1 (MQ=255) cATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTg > 1:2843988/1‑71 (MQ=255) tAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGt > 1:2800879/1‑71 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg < 1:2485013/70‑1 (MQ=255) aCGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTg > 1:1143899/1‑70 (MQ=255) aaTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:4910924/1‑54 (MQ=255) aTGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:1341780/61‑1 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:569442/1‑47 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATg > 1:2407182/1‑47 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:287165/70‑1 (MQ=255) cTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCAt < 1:3173011/70‑1 (MQ=255) ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:3424891/1‑65 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:71809/1‑47 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:4162378/70‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTa > 1:2451728/1‑46 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:2969026/1‑71 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:152248/1‑71 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGGTg > 1:723710/1‑55 (MQ=255) tttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTAt > 1:2756339/1‑46 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:804596/1‑52 (MQ=255) ttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt > 1:5106035/1‑52 (MQ=255) tACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATAc > 1:4696342/1‑71 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:5317859/70‑1 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:5661682/70‑1 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGaa > 1:1195276/1‑52 (MQ=255) cGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGa > 1:1911385/1‑70 (MQ=255) tCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGt < 1:3926415/39‑1 (MQ=37) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:1302562/40‑1 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGtt < 1:4728297/40‑1 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:4976200/55‑1 (MQ=255) tCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:5327225/55‑1 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:214042/1‑71 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4480796/1‑71 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTT‑GTAAACAGCATAATCATTCGGt > 1:3371417/1‑61 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAtt > 1:1258370/1‑56 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1121669/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1549438/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4613870/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1222664/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:4542885/1‑70 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:3372839/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:1871851/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACa > 1:195424/1‑46 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:2515203/1‑71 (MQ=255) ccGGGAAGAGAATAACGTCGCGAATAGTATGGCTGt > 1:4589784/1‑36 (MQ=37) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:1630853/1‑46 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAg > 1:5293691/1‑46 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCa > 1:2797841/1‑48 (MQ=255) cGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa > 1:2007391/1‑62 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa > 1:166024/1‑67 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc < 1:1912456/71‑1 (MQ=255) aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAcc > 1:4509101/4‑68 (MQ=255) aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa < 1:4105807/67‑1 (MQ=255) aaCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc > 1:605765/1‑68 (MQ=255) agaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGa > 1:4320936/3‑71 (MQ=255) aCAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGa > 1:4756273/1‑71 (MQ=255) cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:2554270/1‑71 (MQ=255) cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:1415903/1‑71 (MQ=255) cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:818567/1‑71 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:3687344/1‑43 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:2029828/1‑53 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc < 1:4848811/66‑1 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:2071754/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:1768795/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:539040/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:602986/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:4361469/1‑71 (MQ=255) gAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc > 1:591399/1‑69 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:1113569/1‑70 (MQ=255) aaTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAccag > 1:3842704/1‑69 (MQ=255) aTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGa > 1:4995649/1‑66 (MQ=255) aTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc > 1:4415567/1‑67 (MQ=255) tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:5687052/71‑1 (MQ=255) tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:3501053/71‑1 (MQ=255) aCGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAtac > 1:3015437/1‑56 (MQ=255) cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt < 1:5224403/56‑1 (MQ=255) gTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCa > 1:2743370/1‑52 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1262694/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:4781095/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1028680/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:405258/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg < 1:2874951/66‑2 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg < 1:65892/66‑2 (MQ=255) cgcgAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg < 1:4651124/65‑2 (MQ=255) gcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc < 1:1094365/55‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAGTTCCCAGACCTGCTGTcggcgg < 1:2206873/70‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg < 1:2438293/60‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt < 1:834487/71‑1 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATAc > 1:2037821/1‑35 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct > 1:1153979/1‑61 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt > 1:561937/1‑70 (MQ=255) | CGGGGTTGTGAGCATAACGTAATGCTTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGT > minE/1932900‑1933036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |