| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,932,994 | T→A | 29.4% | V483V (GTA→GTT) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,932,994 | 0 | T | A | 29.4% | 225.0 / 119.9 | 146 | V483V (GTA→GTT) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (62/41); new base A (30/13); total (92/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.48e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01 | |||||||||||
Only 100 of 147 total aligned reads displayed.
TTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAA > minE/1932925‑1933063 | ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:2810753/1‑71 (MQ=255)ttATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:80001/1‑71 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:4162378/70‑1 (MQ=255) aTTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:1140601/70‑1 (MQ=255) ttttACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCa > 1:2969026/1‑71 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:469845/70‑1 (MQ=255) ccGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACg < 1:5317859/70‑1 (MQ=255) gACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTc > 1:4596429/1‑70 (MQ=255) cGCATCGCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGa > 1:1911385/1‑70 (MQ=255) tCGCCGGTAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTAc < 1:2761608/55‑1 (MQ=255) cGCCGGGAAGAGAATAACGTCGCGAATAGTATGACTGTT‑GTAAACAGCATAATCATTCGGt > 1:3371417/1‑61 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCAtt > 1:1258370/1‑56 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1121669/1‑70 (MQ=255) gCCGGGAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCaa > 1:1549438/1‑70 (MQ=255) gCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAt > 1:596568/1‑71 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:615725/1‑56 (MQ=255) ccGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATa > 1:1964495/1‑56 (MQ=255) cGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGa > 1:2007391/1‑62 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa > 1:166024/1‑67 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCaa > 1:538936/1‑67 (MQ=255) gggAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTcc < 1:1912456/71‑1 (MQ=255) ggAAGAGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1215495/64‑1 (MQ=255) aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATAcc > 1:4509101/4‑68 (MQ=255) aagaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCa < 1:4105807/67‑1 (MQ=255) agaGAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGa > 1:4320936/3‑71 (MQ=255) cAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:2554270/1‑71 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:3687344/1‑43 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:3349004/1‑43 (MQ=255) aGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTa > 1:4210291/1‑43 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:539040/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:4361469/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:2071754/1‑71 (MQ=255) gAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:602986/1‑71 (MQ=255) gAATAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc > 1:591399/1‑69 (MQ=255) aaTAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:1113569/1‑70 (MQ=255) aaTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAccag > 1:3842704/1‑69 (MQ=255) aTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGa > 1:4995649/1‑66 (MQ=255) aTAACGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGAc > 1:4415567/1‑67 (MQ=255) tAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct < 1:5687052/71‑1 (MQ=255) aCGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt < 1:5310255/57‑1 (MQ=255) aCGTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAAtac > 1:3015437/1‑56 (MQ=255) cGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAAtt < 1:5224403/56‑1 (MQ=255) gTCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCa > 1:4889263/1‑52 (MQ=255) tCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg < 1:5073843/71‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:4781095/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1028680/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:1262694/50‑1 (MQ=255) tCGCGAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATAcc < 1:405258/50‑1 (MQ=255) cgcgAATAGTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcg < 1:4651124/65‑2 (MQ=255) gcgGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTccc < 1:1094365/55‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAGTTCCCAGACCTGCTGTcggcgg < 1:2206873/70‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctg < 1:2438293/60‑1 (MQ=255) ggATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt < 1:834487/71‑1 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATAc > 1:2037821/1‑35 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACctgct > 1:1153979/1‑61 (MQ=255) gATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGt > 1:1655341/1‑70 (MQ=255) atagTATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtgg > 1:3781893/4‑65 (MQ=255) gTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGc > 1:1587734/1‑70 (MQ=255) tATGGCTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg < 1:2880802/57‑3 (MQ=255) tGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt < 1:5646041/70‑1 (MQ=255) gCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGtt > 1:259517/1‑71 (MQ=255) cTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTc < 1:2287609/71‑1 (MQ=255) cTGTTAGTAAACAGCATAATCATTCGGTCGATACCAATACCCAGACcagcgg > 1:3006644/1‑50 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTTCTCTCGGCGGTAAGCCATGTTCCa < 1:227321/70‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTa > 1:4117786/1‑58 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTa > 1:4531051/1‑58 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:692037/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:5648073/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:2511018/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:1677124/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTcc < 1:498485/69‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCa < 1:2188498/70‑1 (MQ=255) ttGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAg > 1:249944/1‑71 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:639598/1‑41 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg > 1:3662225/1‑71 (MQ=255) ggTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg > 1:4782943/1‑71 (MQ=255) gTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTg < 1:4421421/70‑1 (MQ=255) tGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:837025/1‑71 (MQ=255) aaaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag > 1:2897954/2‑69 (MQ=255) aaaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTTGGCGGCAGACCATATTCCAgcgcag > 1:3994867/2‑69 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:4682425/1‑37 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:4576652/1‑37 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:4574457/1‑37 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGAc > 1:4565460/1‑37 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt < 1:4710148/71‑1 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt < 1:244594/71‑1 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt < 1:3268170/71‑1 (MQ=255) aaCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGt < 1:3781630/71‑1 (MQ=255) aaCAGCATAATCATTCGGTCGATACCAATACCCAGa > 1:4090682/1‑36 (MQ=16) aaCAGCATAATCATTCGGTCGATACCAATACCCAGACCAGCGGTtggcggca > 1:2556513/1‑50 (MQ=255) aCAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTaa < 1:4545840/52‑1 (MQ=255) cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTcgg > 1:1046314/1‑45 (MQ=255) cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt > 1:5240619/1‑56 (MQ=255) cAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCAt > 1:4860255/1‑56 (MQ=255) aGCATTACCATACGGTCGATACCAATTCCCAGACct > 1:2953900/1‑36 (MQ=255) gCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:3103808/1‑65 (MQ=255) gCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:1486122/1‑65 (MQ=255) gCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCg > 1:1454453/1‑65 (MQ=255) cATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACg > 1:379263/1‑70 (MQ=255) tACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTaa > 1:1038074/1‑70 (MQ=255) | TTATTTTACCGGACGCATCGCCGGGAACAGAATAACGTCGCGGATGGTATGGCTGTTGGTGAACAGCATTACCATACGGTCGATACCAATTCCCAGACCTGCTGTCGGCGGTAAGCCATGTTCCAGTGCGGTGACGTAA > minE/1932925‑1933063 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |