| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 310,794 | T→A | 8.5% | E693D (GAA→GAT) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 310,794 | 0 | T | A | 8.5% | 158.6 / 6.4 | 59 | E693D (GAA→GAT) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base T (23/31); new base A (3/2); total (26/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.46e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01 | |||||||||||
GTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTG > minE/310724‑310864 | gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt < 1:2909431/71‑1 (MQ=255)gTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt < 1:5607459/71‑1 (MQ=255) cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGtttt < 1:4161674/68‑1 (MQ=255) cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg > 1:3586773/1‑71 (MQ=255) cGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg > 1:3162654/1‑71 (MQ=255) gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt < 1:1106219/71‑1 (MQ=255) gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt < 1:4482940/71‑1 (MQ=255) gCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt < 1:5023481/71‑1 (MQ=255) gTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc < 1:295943/71‑1 (MQ=255) cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCGTGAGTCAGTTTTTCGTGAcc > 1:280878/1‑69 (MQ=255) cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc < 1:3969444/69‑1 (MQ=255) cAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc > 1:4977237/1‑69 (MQ=255) cATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc < 1:83119/71‑1 (MQ=255) cATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGc < 1:5482814/71‑1 (MQ=255) tatCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCa < 1:194227/71‑1 (MQ=255) gTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc < 1:5345261/57‑1 (MQ=255) cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa > 1:5678351/1‑71 (MQ=255) cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCaa < 1:4248127/71‑1 (MQ=255) cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa < 1:2166150/70‑1 (MQ=255) cTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCa < 1:4569373/70‑1 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:1054075/1‑69 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:1785531/1‑69 (MQ=255) cGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:2705732/1‑69 (MQ=255) ctgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTc < 1:4555066/43‑1 (MQ=255) tgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:3442614/1‑71 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGt > 1:1755266/1‑43 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:1410835/1‑70 (MQ=255) ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca > 1:4169904/1‑70 (MQ=255) ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca > 1:2339927/1‑70 (MQ=255) tCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGcc > 1:1967700/1‑54 (MQ=255) cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAc < 1:1587239/42‑1 (MQ=255) cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa < 1:1220595/70‑1 (MQ=255) cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:817166/69‑1 (MQ=255) tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg > 1:3649713/1‑37 (MQ=255) tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCg > 1:1733112/1‑37 (MQ=255) tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc < 1:1351816/42‑1 (MQ=255) tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc < 1:70925/42‑1 (MQ=255) tGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGAcc < 1:5243633/42‑1 (MQ=255) gcaagcaaCTTGTTACGCGCCTGAGTCAGTTTTTCGTGACCa > 1:1807289/1‑42 (MQ=255) gcaagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCa > 1:2489477/1‑42 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc < 1:1626494/70‑1 (MQ=255) aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga < 1:999838/71‑3 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa < 1:2247475/71‑1 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa < 1:1283897/71‑1 (MQ=255) agcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga > 1:1523076/1‑68 (MQ=255) gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa > 1:1711755/1‑70 (MQ=255) gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa > 1:4088067/1‑70 (MQ=255) gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 1:425769/1‑71 (MQ=255) caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc > 1:4575506/1‑71 (MQ=255) caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc > 1:2708401/1‑71 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:5341281/71‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTg < 1:5583754/45‑1 (MQ=255) cTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAAt > 1:436058/1‑51 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:835662/56‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:2018286/56‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:4700272/71‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:4797019/71‑1 (MQ=255) tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:4778008/1‑69 (MQ=255) ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc < 1:577185/71‑1 (MQ=255) ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc < 1:3974914/71‑1 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt < 1:2198278/71‑1 (MQ=255) gTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc < 1:3457405/37‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:2744478/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:3020273/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:4875957/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:383746/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:1198118/53‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc < 1:1731220/54‑1 (MQ=255) ttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGa > 1:2639938/1‑70 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:2049266/1‑41 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:1446223/1‑41 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCa > 1:4645013/1‑53 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGt > 1:4281216/1‑62 (MQ=255) ttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTTCCCAGTTCCACGATTg > 1:3998606/1‑71 (MQ=255) ttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt < 1:2025623/70‑1 (MQ=255) | GTACGCTGGTCAACATATCAGGGTGCTTCGCTGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTG > minE/310724‑310864 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |