| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,204 | G→A | 49.5% | R413R (CGC→CGT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,204 | 0 | G | A | 49.5% | 10.3 / 147.3 | 109 | R413R (CGC→CGT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (30/25); new base A (22/32); total (52/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.81e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 114 total aligned reads displayed.
CTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAA > minE/1933135‑1933274 | cTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc > 1:1817262/1‑71 (MQ=255) tGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCg > 1:3995602/1‑71 (MQ=255) gATCTTCTGCGTCGTTTAATTCGCTAAAACCATTAACGATTTCACGACCACCGATGAAGAATTCAAAACgg > 1:579612/1‑71 (MQ=255) aTCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt > 1:5260041/1‑71 (MQ=255) cTTCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTc > 1:162298/1‑70 (MQ=255) cTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt > 1:83250/1‑71 (MQ=255) ttCTGCGTCGTTTAATTCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTcgg > 1:5093520/1‑69 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGt < 1:3795329/66‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc < 1:4732972/67‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgt < 1:148358/70‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:181014/68‑1 (MQ=255) ccGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCt < 1:3784642/68‑1 (MQ=255) cgcgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCtgtg < 1:5053157/70‑1 (MQ=255) cgTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAtt < 1:1958619/71‑1 (MQ=255) tCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt < 1:2735161/70‑1 (MQ=255) cATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTc < 1:1971917/61‑1 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg > 1:4314297/1‑71 (MQ=255) tCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCggg > 1:2018706/1‑71 (MQ=255) aGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGt > 1:3826387/1‑70 (MQ=255) attCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt < 1:4205209/68‑1 (MQ=255) ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTc < 1:3011458/62‑1 (MQ=255) ttCGCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa > 1:1234982/2‑71 (MQ=255) tCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAAc > 1:3613736/1‑71 (MQ=255) gCTAAAACCATTACCGATTTTACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat < 1:4925232/71‑3 (MQ=255) gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcat < 1:321381/71‑3 (MQ=255) gCTAAAACCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAca < 1:5627787/70‑2 (MQ=255) aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat > 1:2156035/1‑69 (MQ=255) aaaaCCATTACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat > 1:2585388/1‑69 (MQ=255) aGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGc < 1:5349877/42‑1 (MQ=255) gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac > 1:3650974/1‑71 (MQ=255) gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac > 1:3459425/1‑71 (MQ=255) gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac > 1:1998655/1‑71 (MQ=255) gCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac > 1:2357625/1‑71 (MQ=255) cattACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacgg < 1:217585/69‑2 (MQ=255) gTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacgac > 1:169675/1‑71 (MQ=255) ttAGCGATTTCACGACCACCGATGAAGAATTCAAAACg < 1:5633858/38‑1 (MQ=25) ttACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat < 1:4769723/64‑3 (MQ=255) ttACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAttt > 1:1390382/1‑49 (MQ=255) tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa > 1:5626423/1‑45 (MQ=255) tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa > 1:4416406/1‑45 (MQ=255) tACCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa > 1:3099723/1‑45 (MQ=255) gaCCGATTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGa > 1:5177432/2‑45 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGAt > 1:3840602/1‑45 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg > 1:1279968/1‑71 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAcatcat > 1:1087368/1‑56 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5034452/71‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg > 1:610317/1‑71 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:4415099/71‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:784231/71‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5182046/71‑1 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg < 1:4017068/66‑3 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg < 1:4283486/66‑3 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg < 1:1903804/66‑3 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:4345069/71‑1 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:114508/71‑1 (MQ=255) tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc < 1:1902831/71‑1 (MQ=255) tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc < 1:2494489/71‑1 (MQ=255) ttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAAc > 1:1764189/1‑51 (MQ=255) tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGcc > 1:3604451/1‑66 (MQ=255) tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg < 1:5217669/70‑1 (MQ=255) tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg < 1:2327593/70‑1 (MQ=255) cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1954216/69‑1 (MQ=255) cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGGCAGCGgag > 1:4861846/1‑70 (MQ=255) cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgag > 1:2485440/1‑70 (MQ=255) gACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCTTGCCAGCGgggac < 1:4978718/71‑2 (MQ=255) accaccAATGAAGAACTCTAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTac < 1:5526757/53‑1 (MQ=255) ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc > 1:1245625/1‑71 (MQ=255) accaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa < 1:5295457/67‑1 (MQ=255) gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTaa > 1:168162/2‑38 (MQ=25) gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTg < 1:5022301/70‑1 (MQ=255) actGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt < 1:5183383/68‑1 (MQ=255) aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc > 1:3819135/1‑55 (MQ=255) aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc > 1:5497285/1‑55 (MQ=255) aTGAAGAATTCAAAACGGTCGGTGATTTCCGGGtt < 1:1059066/35‑1 (MQ=25) aTGAAGAATTCAAAACGGGCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGc < 1:162148/71‑1 (MQ=255) gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:1030113/63‑1 (MQ=255) gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:3077125/63‑1 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAAc > 1:3454373/1‑35 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg < 1:3482038/71‑1 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg > 1:2914009/1‑70 (MQ=255) agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:4604216/61‑1 (MQ=255) gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTac < 1:3750045/41‑1 (MQ=19) gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa > 1:2674907/1‑70 (MQ=255) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcg > 1:573003/2‑42 (MQ=16) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacg < 1:188270/38‑1 (MQ=21) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacg < 1:5389028/38‑1 (MQ=21) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:1416983/47‑1 (MQ=19) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5411983/47‑1 (MQ=19) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:2235573/47‑1 (MQ=19) tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg > 1:4740757/1‑64 (MQ=255) tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg > 1:2266241/1‑64 (MQ=255) tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTc > 1:5697636/1‑71 (MQ=255) cAAAACGGTCGGTGATTTCCGGGTTAACATCATTacgg > 1:5282043/1‑37 (MQ=16) aaaaCGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTccg > 1:4684276/1‑69 (MQ=255) aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa > 1:1058383/1‑64 (MQ=255) aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:4494449/71‑1 (MQ=255) aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:5057228/71‑1 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:2978820/1‑39 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:1776076/1‑39 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:766922/1‑39 (MQ=255) | CTGATCTTCCGCGTCATTCAGCTCGCTAAAGCCGTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAA > minE/1933135‑1933274 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |