| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,237 | A→G | 50.5% | R402R (CGT→CGC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,237 | 0 | A | G | 50.5% | ‑3.9 / 127.1 | 95 | R402R (CGT→CGC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (20/27); new base G (19/29); total (39/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 104 total aligned reads displayed.
GTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCC > minE/1933168‑1933306 | gTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTacgac > 1:169675/1‑71 (MQ=255) aCCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGAcgcg > 1:1279968/1‑71 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:4415099/71‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5034452/71‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5182046/71‑1 (MQ=255) aTTTCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg > 1:610317/1‑71 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg < 1:1903804/66‑3 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg < 1:4283486/66‑3 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcgtg < 1:4017068/66‑3 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:114508/71‑1 (MQ=255) tttCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGc < 1:4345069/71‑1 (MQ=255) tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc < 1:2494489/71‑1 (MQ=255) tttCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGc < 1:1902831/71‑1 (MQ=255) tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGcc > 1:3604451/1‑66 (MQ=255) tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg < 1:5217669/70‑1 (MQ=255) tCACGACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCg < 1:2327593/70‑1 (MQ=255) cACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1954216/69‑1 (MQ=255) cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGGCAGCGgag > 1:4861846/1‑70 (MQ=255) cGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGgag > 1:2485440/1‑70 (MQ=255) gACCACCGATGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCTTGCCAGCGgggac < 1:4978718/71‑2 (MQ=255) ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc > 1:1928819/1‑71 (MQ=255) ccaccaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc > 1:1245625/1‑71 (MQ=255) accaATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGaa < 1:5295457/67‑1 (MQ=255) gaTGAAGAATTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTg < 1:5022301/70‑1 (MQ=255) actGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt < 1:5183383/68‑1 (MQ=255) aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc > 1:5497285/1‑55 (MQ=255) aaTGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGcc > 1:3819135/1‑55 (MQ=255) aTGAAGAATTCAAAACGGGCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGc < 1:162148/71‑1 (MQ=255) tGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCt < 1:3702050/68‑1 (MQ=255) gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:1030113/63‑1 (MQ=255) gaagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:3077125/63‑1 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCgg < 1:3482038/71‑1 (MQ=255) aagaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCg > 1:2914009/1‑70 (MQ=255) agaaCTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:4604216/61‑1 (MQ=255) gaaTTCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa > 1:2674907/1‑70 (MQ=255) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTacggcg > 1:573003/2‑42 (MQ=16) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:2235573/47‑1 (MQ=19) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:5411983/47‑1 (MQ=19) ttCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:1416983/47‑1 (MQ=19) tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg > 1:4740757/1‑64 (MQ=255) tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCg > 1:2266241/1‑64 (MQ=255) tCAAAACGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTc > 1:5697636/1‑71 (MQ=255) cAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATAtt > 1:4524972/1‑69 (MQ=255) aaaaCGGTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTccg > 1:4684276/1‑69 (MQ=255) aaaGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGa > 1:1058383/1‑64 (MQ=255) aacggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTa < 1:5613074/69‑1 (MQ=255) aacggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTa < 1:2695751/69‑1 (MQ=255) aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:5057228/71‑1 (MQ=255) aGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTa < 1:4494449/71‑1 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:766922/1‑39 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:1776076/1‑39 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACgcgc > 1:2978820/1‑39 (MQ=255) gCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTaa > 1:2623724/1‑71 (MQ=255) cggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAg < 1:3392334/41‑1 (MQ=19) cggtcggtGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGggga > 1:3525716/1‑47 (MQ=19) cGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAAt < 1:2902075/71‑1 (MQ=255) ggTCGGTGATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGata > 1:3379239/1‑61 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:1272112/38‑1 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCg < 1:5655235/38‑1 (MQ=255) tgtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt < 1:3115275/47‑1 (MQ=255) ggtgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGa > 1:546466/2‑55 (MQ=255) gtgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGt > 1:4739304/1‑71 (MQ=255) gtgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGcc < 1:3685336/51‑1 (MQ=255) gtgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGcc < 1:4504936/51‑1 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGatat < 1:2685242/56‑1 (MQ=255) tgATTTCCGGGTTAACGTCGTTACGACGCGCAAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTc > 1:4597413/1‑71 (MQ=255) tgATTTCCGGGTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt < 1:310383/71‑2 (MQ=255) aTTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACtt > 1:1742510/1‑43 (MQ=255) ccGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAAc < 1:645268/37‑1 (MQ=255) gggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGa < 1:2620717/69‑1 (MQ=255) gggTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGa < 1:3645299/69‑1 (MQ=255) gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAAt > 1:5534655/1‑69 (MQ=255) gTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCa < 1:4157015/71‑1 (MQ=255) gTTAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:5486266/71‑1 (MQ=255) ttAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAg < 1:5676097/71‑1 (MQ=255) ttAACATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAg < 1:2243293/71‑1 (MQ=255) aaCGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGa < 1:1599594/70‑1 (MQ=255) aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa > 1:5617751/1‑65 (MQ=255) aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGaa > 1:1530850/1‑65 (MQ=255) aaCATCATTACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAt > 1:4055516/1‑71 (MQ=255) cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:2597515/50‑1 (MQ=255) cgtcgtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATg < 1:3767432/50‑1 (MQ=255) tcattACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGa > 1:5360930/4‑66 (MQ=255) tcattACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGAtg < 1:2922824/65‑1 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt > 1:2449454/1‑71 (MQ=255) gtTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCtt < 1:3358170/71‑1 (MQ=255) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAg > 1:4785117/2‑49 (MQ=19) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAg > 1:580606/2‑49 (MQ=19) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt > 1:2834892/2‑51 (MQ=19) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGtt > 1:4030066/2‑51 (MQ=19) attACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCtt < 1:187248/70‑1 (MQ=255) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATaaa < 1:2350342/47‑1 (MQ=19) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg < 1:3570228/52‑3 (MQ=19) ttACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTc > 1:2088010/1‑71 (MQ=255) tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:4351435/60‑1 (MQ=255) tACGGCGTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCa < 1:40475/60‑1 (MQ=255) acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg > 1:2387713/1‑48 (MQ=19) acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGttg > 1:638248/1‑48 (MQ=19) acggcgTGCCAGCGGGGACACTTCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTg > 1:3141325/1‑71 (MQ=255) gacgCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGcc < 1:3442001/71‑1 (MQ=255) | GTTACCGATTTCACGACCACCAATGAAGAACTCAAAGCGGTCTGTGATTTCCGGGTTAACGTCGTTACGACGCGCCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCC > minE/1933168‑1933306 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |