| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,312 | T→A | 30.4% | E377D (GAA→GAT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,312 | 0 | T | A | 30.4% | 239.8 / 139.9 | 161 | E377D (GAA→GAT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (55/57); new base A (21/28); total (76/85) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.96e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.15e-01 | |||||||||||
Only 100 of 175 total aligned reads displayed.
CCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGC > minE/1933242‑1933380 | gCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACttcttc > 1:137987/1‑70 (MQ=255) aaaCTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc < 1:3332629/71‑1 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg < 1:157479/65‑1 (MQ=255) cTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg < 1:5651827/65‑1 (MQ=255) ttCTGCCGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctctg > 1:1675878/1‑69 (MQ=255) ttCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCgg > 1:659157/1‑71 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:5461863/1‑65 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:4072003/1‑65 (MQ=255) tCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAt > 1:2316289/1‑65 (MQ=255) cTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGt > 1:1264060/1‑70 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:3331715/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:5257695/70‑1 (MQ=255) ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:4175604/70‑1 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:1224416/71‑3 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:3572823/71‑3 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:3931061/71‑3 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa < 1:2303409/55‑1 (MQ=255) atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc > 1:2914735/1‑71 (MQ=255) atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc > 1:5397678/1‑71 (MQ=255) ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:248214/66‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGa < 1:5684663/49‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3587386/1‑71 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:880591/3‑70 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCg > 1:4408036/1‑56 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:1606530/1‑70 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:4073074/2‑69 (MQ=255) aGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa < 1:3773351/71‑1 (MQ=255) taataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACttcatca < 1:2489814/44‑2 (MQ=37) taataaAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCaac < 1:2622100/71‑3 (MQ=255) aaTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAg < 1:2172081/46‑1 (MQ=255) aaTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGa < 1:3029277/70‑1 (MQ=255) aaTGAAGCTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc > 1:3738040/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:3130815/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:5092796/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:2666226/1‑71 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGAtc < 1:3058260/47‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:171847/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:4639703/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc < 1:3814991/64‑1 (MQ=255) aaGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGAtctct > 1:1996093/1‑47 (MQ=37) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:2208617/1‑57 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:1942019/1‑69 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:5525834/1‑69 (MQ=255) aGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAg < 1:375750/71‑1 (MQ=255) tggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGcttt > 1:2315922/2‑69 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:2444210/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:4098982/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGctct < 1:3906577/70‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAg < 1:5275188/36‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAg < 1:4993804/36‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAg < 1:2047972/36‑1 (MQ=37) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa > 1:1904783/1‑65 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa > 1:3610316/1‑65 (MQ=255) gAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGtga > 1:4453235/1‑40 (MQ=25) gAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGtga > 1:139788/1‑40 (MQ=25) gAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGcttt > 1:3166104/1‑64 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:3421519/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:1853951/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:1493536/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:2721236/69‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc < 1:3894561/56‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:4173209/68‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2143698/42‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:215251/42‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc > 1:336830/1‑57 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc > 1:2997193/1‑57 (MQ=255) tCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:4731100/42‑3 (MQ=21) tCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAcc > 1:4148008/1‑70 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2003497/41‑1 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1590314/1‑68 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc < 1:133440/47‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGt < 1:2902844/70‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:4659103/1‑71 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:2546909/71‑1 (MQ=255) aGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAccgt < 1:3852109/71‑4 (MQ=255) gATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg < 1:4687913/71‑1 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:5369291/71‑1 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:5632467/71‑1 (MQ=255) tgtgCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCa < 1:1279444/46‑1 (MQ=19) tgtgCTTCTGCCACTTCATCAAAGATCTCTGTAACAATACGTCCCa < 1:4562239/46‑1 (MQ=25) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:662941/1‑38 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:471681/1‑38 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:1040531/1‑45 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtcttc > 1:4011272/1‑59 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:2393523/1‑71 (MQ=255) tgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg > 1:1089477/1‑70 (MQ=255) tgCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAcc < 1:3678908/64‑2 (MQ=255) gCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:3461882/60‑1 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg > 1:347108/1‑65 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1034932/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1536290/1‑70 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:2966395/1‑40 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGCCCCCAGCtcttc > 1:2372027/1‑53 (MQ=255) aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtctt > 1:5169978/1‑47 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:5553026/1‑69 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:2793419/1‑70 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:3662298/70‑1 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:697422/70‑1 (MQ=255) cttcGAAGATCTAGGTAACGATACTGCCAAGACCCCAGct > 1:4581491/1‑40 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:1316710/64‑1 (MQ=255) | CCAGCGGAGAAACTTCTGCCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGC > minE/1933242‑1933380 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |