| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,327 | A→G | 24.6% | V372V (GTT→GTC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,327 | 0 | A | G | 24.6% | 250.4 / 85.4 | 146 | V372V (GTT→GTC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base A (56/54); new base G (16/20); total (72/74) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 146 total aligned reads displayed.
CCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAG > minE/1933260‑1933396 | ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:4175604/70‑1 (MQ=255)ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:3331715/70‑1 (MQ=255)ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:5257695/70‑1 (MQ=255)ccGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAAc < 1:2198002/70‑1 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:3931061/71‑3 (MQ=255) cGGATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:1224416/71‑3 (MQ=255) ggATATTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAAt < 1:2151697/71‑1 (MQ=255) ggATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:4125749/71‑1 (MQ=255) atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc > 1:2914735/1‑71 (MQ=255) atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc < 1:1503976/71‑1 (MQ=255) atatTCCGTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATAc > 1:5397678/1‑71 (MQ=255) ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:3361860/66‑1 (MQ=255) ttCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGAt < 1:248214/66‑1 (MQ=255) tCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:3587386/1‑71 (MQ=255) ccgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTcc > 1:228746/3‑70 (MQ=255) cAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc > 1:1606530/1‑70 (MQ=255) cgTAATAAAGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTccc < 1:5349380/69‑1 (MQ=255) aaTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGa < 1:3029277/70‑1 (MQ=255) aaTGAAGCTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc > 1:3738040/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:3130815/1‑71 (MQ=255) aTGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc > 1:2666226/1‑71 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:171847/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:1088435/57‑1 (MQ=255) tGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc < 1:3814991/64‑1 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:2208617/1‑57 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:450541/1‑57 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:4037722/1‑57 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:25551/1‑64 (MQ=255) aaGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAccc > 1:5525834/1‑69 (MQ=255) aGGTTGGCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAg < 1:375750/71‑1 (MQ=255) tggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGcttt > 1:2315922/2‑69 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:4098982/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:2444210/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAc < 1:58183/61‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGctct < 1:3906577/70‑1 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa > 1:3610316/1‑65 (MQ=255) ggCTGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCa > 1:1904783/1‑65 (MQ=255) gCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:3830996/48‑1 (MQ=255) tGAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAAt < 1:3573310/47‑1 (MQ=25) gAATCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGcttt > 1:3166104/1‑64 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc > 1:1300250/1‑59 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:3421519/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:3710205/70‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:1853951/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:2721236/69‑1 (MQ=255) aaTCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc < 1:1493536/69‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcc < 1:3894561/56‑1 (MQ=255) aTCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc < 1:4173209/68‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:215251/42‑1 (MQ=255) tCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGAcccc > 1:4681172/1‑57 (MQ=255) tCAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGAcaa < 1:4724694/42‑3 (MQ=21) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGa < 1:2003497/41‑1 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGccc > 1:155765/1‑49 (MQ=255) cAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCaa > 1:1590314/1‑68 (MQ=255) cAGATGTGCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTtctc > 1:50082/1‑66 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGcc < 1:133440/47‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGt < 1:2902844/70‑1 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg > 1:4659103/1‑71 (MQ=255) aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:2546909/71‑1 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:5369291/71‑1 (MQ=255) tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:5632467/71‑1 (MQ=255) tgtgCTTCTGCCACTTCATCAAAGATCTCTGTAACAATACGTCCCa < 1:4562239/46‑1 (MQ=25) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:471681/1‑38 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATa > 1:662941/1‑38 (MQ=255) gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:1040531/1‑45 (MQ=255) tgCTTCTGCCACTTCATCAAAGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTAcc < 1:3678908/64‑2 (MQ=255) gCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCa < 1:3461882/60‑1 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg > 1:347108/1‑65 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:2069040/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:3481753/1‑70 (MQ=255) cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc > 1:1034932/1‑70 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:3955280/1‑40 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:1984331/1‑40 (MQ=255) tCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCa > 1:2966395/1‑40 (MQ=255) cTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGCCCCCAGCtcttc > 1:2372027/1‑53 (MQ=255) gCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTtctc > 1:3801942/1‑53 (MQ=255) aCTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCtctt > 1:5169978/1‑47 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:5553026/1‑69 (MQ=255) cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt > 1:548779/1‑69 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:296842/1‑70 (MQ=255) tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:2793419/1‑70 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:697422/70‑1 (MQ=255) cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc < 1:3662298/70‑1 (MQ=255) cttcGAAGATCTAGGTAACGATACTGCCAAGACCCCAGct > 1:4581491/1‑40 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt < 1:1923287/54‑1 (MQ=255) ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:1316710/64‑1 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt < 1:2928213/63‑1 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:693485/1‑71 (MQ=255) tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt > 1:1137033/1‑71 (MQ=255) cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg > 1:3270839/1‑66 (MQ=255) aaGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGc < 1:5280438/71‑1 (MQ=255) gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt < 1:4023790/71‑1 (MQ=255) aTCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCtt > 1:2622580/1‑70 (MQ=255) ctctGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttta > 1:2088103/1‑69 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCaa < 1:5103850/35‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:1343296/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:476645/67‑1 (MQ=255) ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg < 1:4826876/68‑1 (MQ=255) tAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg < 1:4947452/37‑1 (MQ=255) tAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCagag > 1:3408136/1‑71 (MQ=255) | CCGGATATTCAGTAATGAAGGTCGGCTGAATCAGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAG > minE/1933260‑1933396 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |