Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,362 G→T 8.6% H361N (CAC→AAC) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,3620GT 8.6% 363.1 / 11.8 115H361N (CAC→AAC) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (62/43);  new base T (5/5);  total (67/48)
Fisher's exact test for biased strand distribution p-value = 7.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.85e-01

Only 100 of 123 total aligned reads displayed.

AGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGG  >  minE/1933292‑1933431
                                                                      |                                                                     
aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg                                                                       <  1:3297181/71‑1 (MQ=255)
aGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTg                                                                       >  1:4659103/1‑71 (MQ=255)
 gATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTgg                                                                      <  1:4687913/71‑1 (MQ=255)
   tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt                                                                    <  1:5369291/71‑1 (MQ=255)
   tgtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt                                                                    <  1:5632467/71‑1 (MQ=255)
    gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg                                                                   >  1:2393523/1‑71 (MQ=255)
    gtgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg                                                                   >  1:2899991/1‑71 (MQ=255)
     tgCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATg                                                                   >  1:1089477/1‑70 (MQ=255)
       cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc                                                                 >  1:1034932/1‑70 (MQ=255)
       cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc                                                                 >  1:1875216/1‑70 (MQ=255)
       cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc                                                                 >  1:1536290/1‑70 (MQ=255)
       cTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGcc                                                                 >  1:3481753/1‑70 (MQ=255)
                cttcttCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAtt                                                         >  1:5553026/1‑69 (MQ=255)
                  tcttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg                                                      >  1:296842/1‑70 (MQ=255)
                   cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                                                     <  1:3662298/70‑1 (MQ=255)
                   cttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                                                     <  1:697422/70‑1 (MQ=255)
                    ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt                                                                    <  1:1923287/54‑1 (MQ=255)
                    ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGAt                                                                    <  1:2489382/54‑1 (MQ=255)
                    ttcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt                                                          <  1:1316710/64‑1 (MQ=255)
                     tcGAATATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt                                                  >  1:2191541/1‑71 (MQ=255)
                     tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGAt                                                          <  1:2928213/63‑1 (MQ=255)
                     tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt                                                  >  1:1137033/1‑71 (MQ=255)
                     tcGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAAt                                                  >  1:693485/1‑71 (MQ=255)
                      cGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAg                                                      >  1:3270839/1‑66 (MQ=255)
                        aaGATCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGc                                               <  1:5280438/71‑1 (MQ=255)
                          gATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt                                             <  1:4023790/71‑1 (MQ=255)
                           aTCTCTGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCtt                                             >  1:2622580/1‑70 (MQ=255)
                             ctctGTGACAATACGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttta                                           >  1:2088103/1‑69 (MQ=255)
                                ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           <  1:476645/67‑1 (MQ=255)
                                ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           <  1:1343296/67‑1 (MQ=255)
                                ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg                                          <  1:4826876/68‑1 (MQ=255)
                                ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg                                          >  1:2012272/1‑68 (MQ=255)
                                 gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCaga                                      <  1:5367815/71‑1 (MQ=255)
                                  tAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCagag                                     >  1:3408136/1‑71 (MQ=255)
                                      gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                                                     <  1:3530729/51‑1 (MQ=255)
                                      gATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa                                 <  1:5642619/71‑1 (MQ=255)
                                       aTACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCt                                              >  1:2604011/1‑57 (MQ=255)
                                       aTACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa                                 <  1:5498546/70‑1 (MQ=255)
                                        tACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg                               <  1:3860992/71‑1 (MQ=255)
                                         aCGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCagcat                                    >  1:3577424/1‑62 (MQ=255)
                                         aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa                                        >  1:5191741/1‑61 (MQ=255)
                                         aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg                               >  1:518802/1‑70 (MQ=255)
                                             cccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           >  1:1401028/1‑54 (MQ=255)
                                              ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTg                                                <  1:1940396/48‑1 (MQ=255)
                                              ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                              >  1:4405054/1‑66 (MQ=255)
                                              ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                              >  1:5408165/1‑66 (MQ=255)
                                               cAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa                        <  1:950847/71‑1 (MQ=255)
                                                aGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           <  1:4230118/51‑1 (MQ=255)
                                                 acccccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAgg                      >  1:5018149/3‑70 (MQ=255)
                                                  aCCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                                                     <  1:2405484/39‑1 (MQ=255)
                                                   ccccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCag                                       <  1:4788319/52‑1 (MQ=255)
                                                    cccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCttt                                            <  1:58127/46‑1 (MQ=255)
                                                    cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGc                                                     <  1:2962336/37‑1 (MQ=255)
                                                    cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCtt                                             <  1:1268140/45‑1 (MQ=255)
                                                    cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           >  1:5667421/1‑47 (MQ=255)
                                                    cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           >  1:4195063/1‑47 (MQ=255)
                                                     ccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTcgg                  <  1:4201/71‑3 (MQ=255)
                                                     ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa                                        <  1:5521836/49‑1 (MQ=255)
                                                     ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                <  1:3136553/57‑1 (MQ=255)
                                                     ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                <  1:2878740/57‑1 (MQ=255)
                                                      cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           >  1:4329494/1‑45 (MQ=255)
                                                       aGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGc                 >  1:147769/1‑70 (MQ=255)
                                                        gCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCg                                  <  1:274392/52‑1 (MQ=255)
                                                         ctctTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg                            <  1:2692205/57‑1 (MQ=255)
                                                          tcttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa                                 >  1:695690/1‑51 (MQ=255)
                                                           cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa               >  1:36992/1‑68 (MQ=255)
                                                           cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa               >  1:4641696/1‑68 (MQ=255)
                                                           cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt            >  1:3269418/1‑71 (MQ=255)
                                                            ttctCAACGTGGATGCCTATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg             <  1:692277/69‑1 (MQ=255)
                                                            ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                <  1:2939179/50‑1 (MQ=255)
                                                            ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                <  1:1048956/50‑1 (MQ=255)
                                                             tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag                                       >  1:166091/1‑42 (MQ=255)
                                                             tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa                        >  1:2435580/1‑57 (MQ=255)
                                                             tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCg          >  1:5657053/1‑71 (MQ=255)
                                                             tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTACGCag                                       >  1:5523015/1‑42 (MQ=255)
                                                              ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt            <  1:377594/68‑1 (MQ=255)
                                                              ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg         >  1:4724252/1‑71 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           >  1:3988614/1‑36 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           >  1:2967431/1‑36 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc                                           >  1:3458363/1‑36 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg                               >  1:821347/1‑48 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg                               >  1:1956897/1‑48 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt                             <  1:3285343/50‑1 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt                             <  1:2974341/50‑1 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt                             <  1:4149875/50‑1 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt                             <  1:2378936/50‑1 (MQ=255)
                                                               tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGt        >  1:4995381/1‑71 (MQ=255)
                                                                cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCTGtt       >  1:4926143/1‑71 (MQ=255)
                                                                cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt       >  1:3457524/1‑71 (MQ=255)
                                                                cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCCAAGTTGTCCAGATCCGCCATGTCGGtt       >  1:1021309/1‑71 (MQ=255)
                                                                 aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                >  1:4579636/1‑45 (MQ=255)
                                                                 aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt                              >  1:741757/1‑47 (MQ=255)
                                                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag                                       >  1:213676/1‑36 (MQ=255)
                                                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag                                       >  1:2269137/1‑36 (MQ=255)
                                                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAg                       >  1:1268317/1‑52 (MQ=255)
                                                                   cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg         >  1:3955385/1‑66 (MQ=255)
                                                                     tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg                            <  1:3351043/45‑1 (MQ=255)
                                                                     tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCgg  >  1:518343/1‑71 (MQ=255)
                                                                      ggATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                <  1:3871441/40‑1 (MQ=255)
                                                                      ggATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa                                <  1:860913/40‑1 (MQ=255)
                                                                      |                                                                     
AGATGTGCTTCTGCCACTTCTTCGAAGATCTCGGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGG  >  minE/1933292‑1933431

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: