| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,390 | C→A | 5.5% | A351A (GCG→GCT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,390 | 0 | C | A | 5.5% | 411.9 / 5.1 | 125 | A351A (GCG→GCT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (63/55); new base A (5/2); total (68/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.53e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
GGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGG > minE/1933324‑1933460 | ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:1343296/67‑1 (MQ=255)ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg > 1:3711795/1‑68 (MQ=255)ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg > 1:2012272/1‑68 (MQ=255)ggTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCg < 1:4826876/68‑1 (MQ=255) gTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCaga < 1:5367815/71‑1 (MQ=255) tACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg < 1:3860992/71‑1 (MQ=255) aCGTCCCAACCCCCAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCagcat > 1:3577424/1‑62 (MQ=255) aCGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa > 1:5191741/1‑61 (MQ=255) cccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:1401028/1‑54 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:4405054/1‑66 (MQ=255) ccAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:5408165/1‑66 (MQ=255) cAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCa < 1:950847/71‑1 (MQ=255) aGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc < 1:4230118/51‑1 (MQ=255) gACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGa < 1:4739081/71‑1 (MQ=255) ccccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCag < 1:4788319/52‑1 (MQ=255) cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:4195063/1‑47 (MQ=255) cccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:5667421/1‑47 (MQ=255) ccAGCTTTTCTCTACCGTAATACCGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTcgg < 1:4201/71‑3 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCa < 1:5521836/49‑1 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:3136553/57‑1 (MQ=255) ccAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:2878740/57‑1 (MQ=255) cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:4329494/1‑45 (MQ=255) cAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATc > 1:1890944/1‑68 (MQ=255) aGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGc > 1:147769/1‑70 (MQ=255) gCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCg < 1:274392/52‑1 (MQ=255) ctctTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg < 1:2692205/57‑1 (MQ=255) tcttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGa > 1:695690/1‑51 (MQ=255) cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa > 1:4641696/1‑68 (MQ=255) cttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCa > 1:36992/1‑68 (MQ=255) ttctCAACGTGGATGCCTATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg < 1:692277/69‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:2939179/50‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa < 1:1048956/50‑1 (MQ=255) ttctCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATg < 1:647785/69‑1 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCg > 1:5657053/1‑71 (MQ=255) tctcAACGTGGATGCCGATAGATTCAGCAATTGCTTACGCag > 1:5523015/1‑42 (MQ=255) ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt < 1:377594/68‑1 (MQ=255) ctcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg > 1:4724252/1‑71 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:3988614/1‑36 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTc > 1:2967431/1‑36 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAg > 1:821347/1‑48 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:2378936/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:3285343/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGtt < 1:4149875/50‑1 (MQ=255) tcAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGt > 1:4995381/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCTGtt > 1:4926143/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt > 1:3457524/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt > 1:95204/1‑71 (MQ=255) cAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCCAAGTTGTCCAGATCCGCCATGTCGGtt > 1:1021309/1‑71 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGaa > 1:4579636/1‑45 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:741757/1‑47 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:3205432/1‑47 (MQ=255) aaCGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGt > 1:4585788/1‑47 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:5230752/1‑36 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCag > 1:213676/1‑36 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAg > 1:1268317/1‑52 (MQ=255) cGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCgg > 1:3955385/1‑66 (MQ=255) tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTg < 1:3351043/45‑1 (MQ=255) tGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCgg > 1:518343/1‑71 (MQ=255) aTGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt < 1:3434576/63‑1 (MQ=255) aTGCCGATAGATTCAGCAATTGCTTTCGAAGAGTCGAAGTTGTCCAGATCCGCCATGT‑GGTTTCCGGGCGa < 1:1434767/71‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGt < 1:4489459/57‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGtt < 1:3428074/62‑1 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa > 1:2398714/1‑71 (MQ=255) tGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa < 1:1231949/71‑1 (MQ=255) gCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt < 1:1653556/41‑1 (MQ=255) gCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt < 1:1167094/41‑1 (MQ=255) ccGATAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGat > 1:5072366/1‑70 (MQ=255) aTAGATTCAGCTAATGCTTTAGCAGCATCAAAATTATCCAGGTCGGCCATATCGGTTTCTGGACGATAttt > 1:2745621/1‑71 (MQ=255) aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGt > 1:1935151/1‑37 (MQ=255) aTAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATAtt > 1:4161495/1‑70 (MQ=255) aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATc > 1:1927055/1‑42 (MQ=255) aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCt > 1:4346231/1‑71 (MQ=255) aGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTACAGa > 1:5257507/1‑40 (MQ=255) gATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt < 1:1784528/71‑1 (MQ=255) aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc < 1:3965987/56‑1 (MQ=255) aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTg < 1:623735/71‑1 (MQ=255) aTTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTg < 1:2807241/71‑1 (MQ=255) ttCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc < 1:5408720/55‑1 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc > 1:1314032/1‑54 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc > 1:1778605/1‑54 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc > 1:1752849/1‑54 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt > 1:5022324/1‑68 (MQ=255) tCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt > 1:254912/1‑71 (MQ=255) cAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGcc > 1:4039130/1‑41 (MQ=255) aaTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCtt > 1:5128272/1‑71 (MQ=255) aaTTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCt > 1:497388/1‑70 (MQ=255) ttGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGt < 1:4502156/43‑1 (MQ=255) ttGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCg < 1:1975309/48‑1 (MQ=255) gCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTcc > 1:3163867/1‑45 (MQ=255) gCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt > 1:725789/1‑62 (MQ=255) gCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACg > 1:4677404/1‑71 (MQ=255) cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCg > 1:3785249/1‑38 (MQ=255) cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCtt > 1:1587487/1‑58 (MQ=255) cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACg < 1:5186628/70‑1 (MQ=255) cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACg < 1:1188248/70‑1 (MQ=255) cTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGc < 1:4297489/71‑1 (MQ=255) ttCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGAt > 1:4093508/1‑59 (MQ=255) tCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGa < 1:1319858/47‑1 (MQ=255) tCGCAGAGTCGAACTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCAt < 1:2628132/70‑1 (MQ=255) cGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATgg > 1:5047525/1‑71 (MQ=255) | GGTAACGATACGGCCCAGACCCCAGCTCTTCTCAACGTGGATGCCGATAGATTCAGCAATTGCTTTCGCAGAGTCGAAGTTGTCCAGATCCGCCATGTCGGTTTCCGGGCGATATTTCTTGATCGCTTCACGCATGG > minE/1933324‑1933460 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |