| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,555 | C→T | 10.0% | S296S (TCG→TCA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,555 | 0 | C | T | 10.0% | 359.0 / 21.4 | 130 | S296S (TCG→TCA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (55/62); new base T (5/8); total (60/70) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.82e-01 | |||||||||||
Only 100 of 131 total aligned reads displayed.
GTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTG > minE/1933486‑1933625 | gTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCg < 1:4424617/71‑1 (MQ=255) tCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGa > 1:3530621/1‑71 (MQ=255) gCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:616503/1‑71 (MQ=255) gTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:4477600/1‑69 (MQ=255) tCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTc < 1:3177385/71‑1 (MQ=255) aCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:861474/1‑66 (MQ=255) aCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:3219102/1‑66 (MQ=255) aCGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:1030118/1‑66 (MQ=255) gTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTc < 1:5471403/63‑1 (MQ=255) gTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTc < 1:4816158/63‑1 (MQ=255) gTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg < 1:2111644/64‑1 (MQ=255) gTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCt > 1:3284125/1‑71 (MQ=255) tCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTc > 1:5632444/1‑71 (MQ=255) cGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCt > 1:5144250/1‑69 (MQ=255) gCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGa > 1:2898334/1‑71 (MQ=255) gCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGa > 1:5327732/1‑71 (MQ=255) gCCGTAGGTCACTTCCGTCTTACCGAGAACATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGa < 1:5013603/71‑1 (MQ=255) cGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCa > 1:2358104/1‑63 (MQ=255) cGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATc < 1:546588/71‑1 (MQ=255) gTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGAt > 1:2143972/1‑69 (MQ=255) tAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAg < 1:2949391/71‑1 (MQ=255) ggTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGa < 1:5094003/70‑1 (MQ=255) gTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCa < 1:529255/58‑1 (MQ=255) gTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCa < 1:4394475/58‑1 (MQ=255) cACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCg > 1:3494102/1‑47 (MQ=255) cACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:494790/1‑52 (MQ=255) cACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:283935/1‑52 (MQ=255) cACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCg > 1:607336/1‑52 (MQ=255) aCTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCg < 1:1604421/46‑1 (MQ=255) aCTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt < 1:5454919/71‑1 (MQ=255) aCTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt > 1:2426161/1‑71 (MQ=255) aCTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt > 1:1174200/1‑71 (MQ=255) ttCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGc < 1:3616657/55‑1 (MQ=255) ttCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGc < 1:3274494/55‑1 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATc > 1:208252/1‑59 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa > 1:3082879/1‑71 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa > 1:4152859/1‑71 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:4282210/70‑1 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:4825934/70‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa < 1:2970151/70‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa < 1:525005/70‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt < 1:5629324/71‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt < 1:4032619/71‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt < 1:1125447/71‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGt < 1:3726233/46‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGt < 1:1097762/46‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt < 1:1070019/63‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt < 1:1550941/71‑1 (MQ=255) ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGc < 1:851229/48‑1 (MQ=255) ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc > 1:2824540/1‑71 (MQ=255) aCCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:750842/61‑1 (MQ=255) aCCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:2674842/61‑1 (MQ=255) agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCa < 1:1806145/40‑1 (MQ=255) agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:251775/1‑71 (MQ=255) aaTATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa < 1:3865103/71‑1 (MQ=255) aaTATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:5144597/1‑71 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt < 1:1886964/58‑1 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:4320873/1‑70 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCg < 1:4707523/39‑1 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGAt < 1:3635940/41‑1 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:3359077/1‑69 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:2809981/1‑69 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:4120994/71‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:2918867/70‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:4458211/70‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa > 1:2636665/1‑69 (MQ=255) tgtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTagag > 1:489102/1‑71 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:4180999/1‑71 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:2505897/1‑71 (MQ=255) ccAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc < 1:2002871/70‑1 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt > 1:5168495/1‑41 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:4668741/1‑58 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:4582410/71‑1 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:4214173/71‑1 (MQ=255) gagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:3095157/70‑1 (MQ=255) agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatc > 1:1944328/1‑70 (MQ=255) agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCAGCGTa > 1:2250359/1‑51 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt < 1:3446813/37‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:3404465/38‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:4564623/38‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:5620026/38‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg < 1:3973897/71‑1 (MQ=255) aCGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:3490659/37‑1 (MQ=255) cGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGt < 1:4610910/71‑1 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3125452/1‑71 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3218272/1‑71 (MQ=255) ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:2298891/71‑1 (MQ=255) ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:1453187/71‑1 (MQ=255) ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:4345748/55‑1 (MQ=255) ggAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg < 1:1628587/71‑1 (MQ=255) gAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:3540865/70‑1 (MQ=255) gAACAGTGACTCTGTCAGTTCAATCAAATCGTAGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:207200/70‑1 (MQ=255) gAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTg > 1:4119688/1‑70 (MQ=255) aaCAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa > 1:4333181/1‑71 (MQ=255) cAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAct > 1:573568/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:3805927/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:2719605/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:4744690/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActc > 1:4926756/1‑70 (MQ=255) cGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTg > 1:4254158/1‑71 (MQ=255) | GTCCAGCGTCACGTCGCCGTAGGTCACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTG > minE/1933486‑1933625 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |