| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 310,823 | G→A | 7.4% | L684L (CTG→TTG) ‡ | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 310,823 | 0 | G | A | 7.4% | 193.9 / 4.2 | 67 | L684L (CTG→TTG) ‡ | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base G (30/32); new base A (3/2); total (33/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.73e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.89e-01 | |||||||||||
TGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCG > minE/310755‑310891 | tgctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:3442614/1‑71 (MQ=255) gctTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCt > 1:1410835/1‑70 (MQ=255) ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca > 1:4169904/1‑70 (MQ=255) ttCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtca > 1:2339927/1‑70 (MQ=255) cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaa < 1:1220595/70‑1 (MQ=255) cTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:817166/69‑1 (MQ=255) ccaagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtc < 1:1626494/70‑1 (MQ=255) aagcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga < 1:999838/71‑3 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa < 1:2247475/71‑1 (MQ=255) aagcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaa < 1:1283897/71‑1 (MQ=255) agcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcga > 1:1523076/1‑68 (MQ=255) gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa > 1:1711755/1‑70 (MQ=255) gcaaCTGGTTACGGGCCTGGGTTAGGGCATCGTGACCCAGCCCAGCCTGATCAATTAACtcaaagtcgaaa > 1:4088067/1‑70 (MQ=255) gcaaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 1:425769/1‑71 (MQ=255) caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc > 1:4575506/1‑71 (MQ=255) caaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGc > 1:2708401/1‑71 (MQ=255) aaCTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:5341281/71‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:2018286/56‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaa < 1:835662/56‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:4797019/71‑1 (MQ=255) gTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:4700272/71‑1 (MQ=255) tACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg > 1:4778008/1‑69 (MQ=255) ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc < 1:3974914/71‑1 (MQ=255) ccTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTAccc < 1:577185/71‑1 (MQ=255) aGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGtt < 1:2198278/71‑1 (MQ=255) gTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCtc < 1:3457405/37‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:383746/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:4875957/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:2744478/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGcc < 1:3020273/48‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:1198118/53‑1 (MQ=255) tttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGc < 1:1731220/54‑1 (MQ=255) ttttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGa > 1:2639938/1‑70 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:1446223/1‑41 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtca > 1:2049266/1‑41 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCa > 1:4645013/1‑53 (MQ=255) tttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGt > 1:4281216/1‑62 (MQ=255) ttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTTCCCAGTTCCACGATTg > 1:3998606/1‑71 (MQ=255) ttCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGAtt < 1:2025623/70‑1 (MQ=255) tCGTGACCAAGGCCAGCCCGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTg < 1:1264835/70‑1 (MQ=255) gTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtcaaag > 1:4482129/1‑40 (MQ=255) gTGACCAAGGCCAGCCTGGTCAATCAGCtcaaagtaaaa > 1:3451915/1‑39 (MQ=255) gTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCgg > 1:134305/1‑71 (MQ=255) gTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCg < 1:5109450/70‑1 (MQ=255) gACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCggg > 1:4076046/1‑70 (MQ=255) gACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGAggg > 1:1973690/1‑70 (MQ=255) aCCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGc < 1:3559267/70‑1 (MQ=255) ccAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTcc > 1:2453408/1‑57 (MQ=255) cAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:76115/42‑1 (MQ=255) aGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGt > 1:4766973/1‑70 (MQ=255) gCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCa > 1:1194664/1‑40 (MQ=255) gCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTaa < 1:2540955/71‑1 (MQ=255) gCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTa > 1:4836064/1‑70 (MQ=255) aGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTg < 1:3554108/35‑1 (MQ=255) cTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTACCACGAtt > 1:1809172/1‑52 (MQ=255) gTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGaa < 1:790889/69‑1 (MQ=255) gTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACACAGTTCCACGATTGCGGGCAGGTTAAAGGCGaaaa > 1:1608180/1‑71 (MQ=255) aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGaaaa > 1:1431125/1‑68 (MQ=255) aaTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTCCCACGATTGCGGGCAGGTTAAAGGCGaaaa > 1:4461151/1‑68 (MQ=255) aTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt < 1:3107834/71‑1 (MQ=255) aTCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt < 1:3114386/71‑1 (MQ=255) tCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCAt < 1:5438985/70‑1 (MQ=255) tCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATc < 1:2605012/71‑1 (MQ=255) tCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATc > 1:1425093/1‑71 (MQ=255) aGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGc > 1:2873737/1‑44 (MQ=255) aGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCggg > 1:5109824/1‑47 (MQ=255) aGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCg < 1:483712/70‑1 (MQ=255) | TGCTTCTGCAAGCAACTGGTTACGCGCCTGAGTCAGTTTTTCGTGACCAAGGCCAGCCTGGTCAATCAGCTCAAAGTCAAAGCCGGTTGCAGTACCCAGTTCCACGATTGCGGGCAGGTTAAAGGCGAAAACCATCG > minE/310755‑310891 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |