| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,579 | T→G | 16.9% | K288N (AAA→AAC) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,579 | 0 | T | G | 16.9% | 211.0 / 47.7 | 112 | K288N (AAA→AAC) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (45/48); new base G (8/11); total (53/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.02e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 123 total aligned reads displayed.
CACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAA > minE/1933510‑1933643 | cACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt > 1:2054902/1‑71 (MQ=255) aCTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt > 1:1174200/1‑71 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa > 1:4152859/1‑71 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa > 1:3082879/1‑71 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa > 1:2175117/1‑71 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:4825934/70‑1 (MQ=255) ccGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:4282210/70‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa < 1:525005/70‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa < 1:2970151/70‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt < 1:1125447/71‑1 (MQ=255) cGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAAt < 1:4032619/71‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt < 1:1569337/63‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCtt < 1:1070019/63‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt < 1:4385361/71‑1 (MQ=255) tCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt < 1:1550941/71‑1 (MQ=255) ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGt > 1:363106/1‑64 (MQ=255) ttACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGc > 1:2824540/1‑71 (MQ=255) aCCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:2674842/61‑1 (MQ=255) agaATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGc > 1:251775/1‑71 (MQ=255) aaTATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa < 1:3865103/71‑1 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCt < 1:1886964/58‑1 (MQ=255) atCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:4320873/1‑70 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:3359077/1‑69 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATg > 1:2809981/1‑69 (MQ=255) tCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa < 1:4120994/71‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:4458211/70‑1 (MQ=255) cTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:1164551/70‑1 (MQ=255) tgtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTagag > 1:489102/1‑71 (MQ=255) tgtgCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTa < 1:4200930/50‑1 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:4180999/1‑71 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:2505897/1‑71 (MQ=255) gtgCCAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGt > 1:2978081/1‑71 (MQ=255) ccAGGGTGCGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc < 1:2002871/70‑1 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt > 1:5168495/1‑41 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:4668741/1‑58 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCa > 1:4795297/1‑58 (MQ=255) agagTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:4214173/71‑1 (MQ=255) agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcatc > 1:1944328/1‑70 (MQ=255) agTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCAGCGTa > 1:2250359/1‑51 (MQ=255) gTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTaa > 1:2766433/1‑42 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCttt < 1:3446813/37‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:3404465/38‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:5620026/38‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:4564623/38‑1 (MQ=255) tACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATg < 1:3973897/71‑1 (MQ=255) aCGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTg < 1:3490659/37‑1 (MQ=255) cGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGt < 1:4610910/71‑1 (MQ=255) cGGAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGt < 1:5607190/71‑1 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3125452/1‑71 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3174490/1‑71 (MQ=255) cGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt > 1:3218272/1‑71 (MQ=255) ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:2298891/71‑1 (MQ=255) ggAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:1453187/71‑1 (MQ=255) gAACAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATtgtg < 1:3540865/70‑1 (MQ=255) aaCAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGaa > 1:4333181/1‑71 (MQ=255) aaCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGaa < 1:655701/71‑1 (MQ=255) aCAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGt < 1:4098631/67‑1 (MQ=255) cAGTGACTCTGTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAAct < 1:4462152/71‑1 (MQ=255) cAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAct > 1:573568/1‑71 (MQ=255) aGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActc > 1:3809577/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:2719605/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:3805927/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct > 1:5368644/1‑71 (MQ=255) gCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActc > 1:4926756/1‑70 (MQ=255) cGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTg > 1:4254158/1‑71 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:4052027/1‑49 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTagag > 1:2863214/1‑49 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTag < 1:1526961/47‑1 (MQ=255) aTTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTgg > 1:1752748/1‑70 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATTGTGAAct < 1:4907958/63‑1 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAAct < 1:2997913/63‑1 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:724880/1‑71 (MQ=255) cGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTa > 1:524734/1‑71 (MQ=255) tgTCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTAt > 1:4743265/2‑71 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATg < 1:658033/71‑1 (MQ=255) gTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATg < 1:3003014/71‑1 (MQ=255) tCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTc > 1:1794025/1‑46 (MQ=255) tCAGTTCAATCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCcatcatt < 1:4831082/53‑2 (MQ=255) tCAGTTCAATCAAATAGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTa < 1:3702415/68‑1 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:1631772/1‑71 (MQ=255) aGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg > 1:2144389/1‑71 (MQ=255) tCGATCAGATCTTTGTAATCTGCGTAAGCCATGTa > 1:168352/1‑35 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:249314/56‑1 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:3499983/56‑1 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAActct < 1:290926/56‑1 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTggg > 1:4682924/1‑59 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:5580556/1‑71 (MQ=255) cGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCAATCATGGTGAACTCTggg > 1:2521287/1‑59 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTAc < 1:2618209/69‑1 (MQ=255) gATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTAc < 1:4813837/69‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg < 1:3696514/46‑1 (MQ=255) aTCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATgg < 1:3918940/46‑1 (MQ=255) taaGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2749264/68‑1 (MQ=255) tCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa > 1:5617105/1‑70 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:5137049/36‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCcat < 1:4623723/36‑1 (MQ=255) aTCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGaa < 1:1727524/45‑1 (MQ=255) cTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACg > 1:3107546/1‑62 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:5237807/57‑1 (MQ=255) tttGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACg < 1:1190534/57‑1 (MQ=255) | CACTTCCGTCTTACCGAGAATATCCTGTGCCAGAGTACGGAACAGCGATTCGGTCAGCTCGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAA > minE/1933510‑1933643 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |