| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 5,581 | G→A | 9.8% | intergenic (+51/+102) | yaaX → / ← yaaA | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 5,581 | 0 | G | A | 9.8% | 165.5 / 6.9 | 62 | intergenic (+51/+102) | yaaX/yaaA | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (32/23); new base A (3/3); total (36/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
CAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGG > minE/5511‑5650 | cAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTgg > 1:4560764/1‑71 (MQ=255) aCCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCa < 1:82975/71‑1 (MQ=255) aaaTGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa > 1:4630571/1‑71 (MQ=255) gACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGt > 1:3674851/1‑70 (MQ=255) aCAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGtt < 1:3212405/70‑1 (MQ=255) aCAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTa < 1:3452523/71‑1 (MQ=255) cAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa < 1:4412898/71‑1 (MQ=255) cAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa > 1:4630314/1‑71 (MQ=255) gCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTg > 1:1866947/1‑71 (MQ=255) ggTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTg < 1:910727/67‑1 (MQ=255) ggTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTg < 1:53032/67‑1 (MQ=255) gTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGTCGCGTCTTAt > 1:2487018/1‑47 (MQ=255) gTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt > 1:3831613/1‑47 (MQ=255) tAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGt > 1:5568036/1‑41 (MQ=255) cgcatccGGTATGTAACGCCTGATGCGACGCTGACGCGTCTTATCTGGCCTACacgctgcgattttgtagg > 1:5540228‑M1/4‑53 (MQ=255) aCAATCAGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAatatat > 1:740861/1‑71 (MQ=255) cAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATAtt < 1:2741661/71‑1 (MQ=255) aTCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTg > 1:5468280/1‑70 (MQ=255) aTCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGa < 1:4792640/71‑1 (MQ=255) cGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAgg > 1:340860/1‑42 (MQ=255) cGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATc > 1:4198018/1‑71 (MQ=255) cGGCATTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggat < 1:3323539‑M1/71‑25 (MQ=255) ggCATTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggat > 1:3154301‑M1/1‑46 (MQ=255) gCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg > 1:1033395/1‑71 (MQ=255) cATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg > 1:3429588/1‑70 (MQ=255) cATTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataag < 1:577759‑M1/71‑28 (MQ=255) ttCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCt < 1:4489936/67‑1 (MQ=255) ttCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataagc > 1:264260‑M1/1‑42 (MQ=255) ttCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccgg > 1:5316084‑M1/1‑42 (MQ=255) tCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt < 1:5617861/39‑1 (MQ=255) tCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCAt > 1:1566045/1‑70 (MQ=255) aacgcctGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataagcaaag > 1:2528465‑M1/3‑39 (MQ=255) aGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCt > 1:626255/1‑71 (MQ=255) acgccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttg < 1:1843152‑M1/51‑15 (MQ=255) acgccTGATGCGACGCTGACGCGTCTTATCTGGCCTACacgctgcgattttgtaggccggataagcaaag < 1:1266928‑M1/69‑33 (MQ=255) gcCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggataagcaaagcgc < 1:270646‑M1/71‑36 (MQ=255) gcCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataagcaaagcg > 1:5269286‑M1/1‑36 (MQ=255) gcCTGATGCGACGCTGACGCGTCTTATCTGGCCTACacgctgcgattttgtaggccggataagcaaagcgc < 1:4307580‑M1/71‑36 (MQ=255) ccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggataagcaaagcgca < 1:1028253‑M1/71‑37 (MQ=255) cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggataagcaaagcgca < 1:3871861‑M1/70‑37 (MQ=255) tGATGCGACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggataagcaaagcgcatc > 1:4757524‑M1/1‑33 (MQ=255) tGATGCGACGCTGACGCGTCTTATCTGGCCTACacgctgcgattttgtaggccgg < 1:2817629‑M1/55‑23 (MQ=255) gATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAg > 1:2140600/1‑71 (MQ=255) gATGCGACGCTGACGCGTCTTATCTGGCCTACacgctgcgattttgtaggccggataagcaaagcgca > 1:3802728‑M1/1‑32 (MQ=255) aTGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg > 1:2157516/1‑71 (MQ=255) tGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtag > 1:1294376‑M1/1‑30 (MQ=255) tGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtag > 1:1451296‑M1/1‑30 (MQ=255) tGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAgg > 1:3454739/1‑70 (MQ=255) gCGACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggataagcaaagcgcatccgg > 1:1124834‑M1/1‑29 (MQ=255) cGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg > 1:4258947/1‑71 (MQ=255) cGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg > 1:4075143/1‑71 (MQ=255) cGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg < 1:5461251/71‑1 (MQ=255) cGACGCTGACGCGTCTTATCTGGCCTACacgctgcgattttgtaggccggataagcaaagcgcatccggca > 1:5385333‑M1/1‑28 (MQ=255) gACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggataagcaaagcgcatccggcat < 1:3128084‑M1/71‑45 (MQ=255) gACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggataagcaaagcgcatccggcat < 1:3170691‑M1/71‑45 (MQ=255) gACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTg < 1:697496/47‑1 (MQ=255) gACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAg > 1:3075199/1‑70 (MQ=255) cGCTGGCGCGTCTTATCAGTCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGAt < 1:2522751/71‑1 (MQ=255) gCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataagcaaagcgcatccggcacg > 1:781071‑M1/1‑24 (MQ=255) cTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataagcaaagcgcatccggcacg < 1:4337157‑M1/68‑46 (MQ=255) cTGGCGCGTCTTATCAGGCCTACacgctgcgattttgta > 1:297465‑M1/1‑23 (MQ=255) cTGGCGCGTCTTATCAGGCCTACacgctgcgattttgta > 1:3077778‑M1/1‑23 (MQ=255) tGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg < 1:4933170/48‑1 (MQ=255) gcgcGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCa > 1:4682794/1‑48 (MQ=255) gcgcGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAgg < 1:2819361/70‑1 (MQ=255) | CAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCTTTTGTAGGCAGGATAAGG > minE/5511‑5650 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |