| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,639 | G→A | 31.6% | S268S (TCC→TCT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,639 | 0 | G | A | 31.6% | 227.1 / 134.1 | 171 | S268S (TCC→TCT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (54/63); new base A (25/29); total (79/92) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01 | |||||||||||
Only 100 of 173 total aligned reads displayed.
CGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACG > minE/1933569‑1933707 | taaGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGa < 1:2749264/68‑1 (MQ=255) tCAAATCGTGGTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa > 1:5617105/1‑70 (MQ=255) aGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:4235832/1‑70 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:4159908/4‑71 (MQ=255) gtggTAATCCGCATACGCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCtt > 1:5217119/4‑71 (MQ=255) tGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCt < 1:4508666/68‑1 (MQ=255) tAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGt < 1:3745819/70‑1 (MQ=255) aTCCGCATACGCCATGTAGAGTTACATCATTGTGAACTCAGGATTATGGCGAACAGaa > 1:4041847/1‑58 (MQ=255) tGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACgg > 1:3010245/1‑70 (MQ=255) acgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:850492/47‑1 (MQ=255) acgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:3607038/47‑1 (MQ=255) aaGCCATGTAGATTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:3969084/1‑71 (MQ=255) aaGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGt < 1:5298862/69‑1 (MQ=255) aaGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa > 1:2642069/1‑71 (MQ=255) cgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTa < 1:2422989/69‑1 (MQ=255) cgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTa < 1:1962743/69‑1 (MQ=255) cgCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTa < 1:2043105/69‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATa > 1:1549854/1‑52 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTa < 1:125228/70‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:2954998/71‑1 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc > 1:4836025/1‑71 (MQ=255) aGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAc < 1:489138/71‑1 (MQ=255) gCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACg < 1:1362355/71‑1 (MQ=255) gCCATGTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACg < 1:4122351/71‑1 (MQ=255) cATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGtt < 1:3032491/66‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:1890924/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:165428/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:5021681/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:4256620/71‑1 (MQ=255) aTGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:3501760/71‑1 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTa > 1:3692779/1‑56 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGa < 1:1720756/60‑1 (MQ=255) tGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGa < 1:4393245/60‑1 (MQ=255) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa < 1:4776305/44‑1 (MQ=16) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGaaa < 1:2916993/44‑1 (MQ=16) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:3789488/42‑1 (MQ=16) gTAGAGTTCCATCATTGTGAACTCAGGATTATGGCGAACAGa < 1:4228840/42‑1 (MQ=16) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:5561247/1‑44 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:4270924/1‑44 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:2373620/1‑44 (MQ=255) gTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:1446979/1‑44 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGaaa > 1:4208479/1‑43 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATAcc < 1:1531179/47‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTg > 1:3513224/1‑69 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:1783731/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:4059263/70‑1 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:5302943/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:3599301/1‑71 (MQ=255) tAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt > 1:176459/1‑71 (MQ=255) gagTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCt > 1:5110416/1‑46 (MQ=255) agTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTc > 1:565703/1‑71 (MQ=255) gTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCg < 1:3840340/71‑1 (MQ=255) ttCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa < 1:671639/71‑1 (MQ=255) ccATCATTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGaa < 1:2076415/70‑1 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGaa > 1:1003256/1‑52 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:3729672/1‑63 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa > 1:2682370/1‑63 (MQ=255) ccATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGAttt > 1:2290000/1‑66 (MQ=255) catcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGtt < 1:4010804/54‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5653391/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5546947/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:4511490/62‑1 (MQ=255) catcatGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGa < 1:5484193/62‑1 (MQ=255) tcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGa > 1:774495/1‑60 (MQ=255) tcatTGTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATacc > 1:2523856/1‑70 (MQ=255) atGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACg > 1:5339757/1‑71 (MQ=255) tgTGAACTCAGGATTATGGCGAACAGAAATACCTTc < 1:3865925/35‑1 (MQ=12) ggTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGa < 1:2793424/62‑1 (MQ=255) gTGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCgtt > 1:1080564/1‑68 (MQ=255) tGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg < 1:1146341/71‑1 (MQ=255) tGAACTCAGGATTATGTCGAACAGAAATACCTTCATTACgg < 1:480592/41‑1 (MQ=255) tGAACTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTca < 1:554714/71‑2 (MQ=255) gAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCg > 1:4582207/1‑70 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGtt < 1:91075/50‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4827354/71‑1 (MQ=255) aaCTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4527674/71‑1 (MQ=255) aaCTCAGGATTATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTcaa > 1:3317304/1‑68 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:2892083/71‑1 (MQ=255) ctctGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc > 1:4649937/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:5368982/42‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGa < 1:675965/65‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:1408252/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:5437985/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGccac > 1:2777119/1‑71 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGc < 1:1602887/68‑1 (MQ=255) tGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTAAGGTTGATTTCGAATACACGCTCGAAGccac > 1:2157608/1‑71 (MQ=255) tGGGTTATCACGTACGGAAATACCTTCGTTACGGAAGTTACg < 1:1613899/42‑1 (MQ=255) ggTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAg > 1:4676412/1‑65 (MQ=255) gattATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAAccgcc > 1:4934209/3‑70 (MQ=255) attATGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCg > 1:2875523/2‑49 (MQ=37) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACg > 1:5447087/1‑36 (MQ=19) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCaaaa > 1:277397/1‑60 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctacaa > 1:748797/1‑69 (MQ=255) aTGGCGAACAGAAATACCTTCATTACGGAAGTTACGGTTGATTTCGAATACCCGTTCAAAACCgcctaca < 1:1084518/70‑2 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAaccac > 1:1326860/1‑69 (MQ=255) aCGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAAccacca < 1:2818589/71‑1 (MQ=255) cGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAg < 1:1516433/71‑1 (MQ=255) tACGGAAATACCTTCGTTACGGAAGTTACGGTTGAt < 1:1002749/36‑1 (MQ=255) aCGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACg > 1:1033978/1‑71 (MQ=255) cGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGaa < 1:4410153/52‑1 (MQ=255) | CGATCAGATCTTTGTAATCTGCGTAAGCCATGTAGAGTTCCATCATGGTGAACTCTGGGTTATGACGTACGGAAATACCTTCGTTACGGAAGTTACGGTTGATTTCGAATACACGCTCGAAGCCACCAACCACCAGACG > minE/1933569‑1933707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |