Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,933,749 G→A 22.0% L232F (CTC→TTC) ‡ lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,933,7490GA22.0% 229.0 / 57.8 118L232F (CTC→TTC) ‡lysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (32/60);  new base A (17/9);  total (49/69)
Fisher's exact test for biased strand distribution p-value = 6.84e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01

Only 100 of 123 total aligned reads displayed.

ACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCG  >  minE/1933681‑1933817
                                                                    |                                                                    
acGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGAt                                                                    <  1:2723919/71‑1 (MQ=255)
 cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCgaga                                                                     <  1:3263218/69‑1 (MQ=255)
 cGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATc                                                                   <  1:2306737/71‑1 (MQ=255)
  gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc                                                                  <  1:925502/71‑1 (MQ=255)
  gCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATcc                                                                  <  1:3558803/71‑1 (MQ=255)
      gAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAg                                                                <  1:1307289/69‑1 (MQ=255)
        aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                            >  1:3754388/1‑71 (MQ=255)
        aGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                            >  1:97304/1‑71 (MQ=255)
         gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                           >  1:1466483/1‑71 (MQ=255)
         gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                           <  1:530615/71‑1 (MQ=255)
         gCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                           <  1:5160679/71‑1 (MQ=255)
            accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                        <  1:1891689/71‑1 (MQ=255)
            accaaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                        >  1:2121995/1‑71 (MQ=255)
              caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                      <  1:5127902/71‑1 (MQ=255)
              caaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATACAGCGCGTTAtgg                                                      <  1:2083800/71‑1 (MQ=255)
               aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAgc                                                               >  1:1710353/1‑61 (MQ=255)
               aaccaCCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtggt                                                     >  1:768292/1‑71 (MQ=255)
                accaccAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAtgg                                                      >  1:1444606/1‑69 (MQ=255)
                acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                    >  1:4548195/4‑71 (MQ=255)
                acaaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTatgatg                                                    >  1:4321830/4‑71 (MQ=255)
                 caaccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAtgatgg                                                   >  1:1068133/3‑71 (MQ=255)
                   accaGACGTTTCAGATACAGCTCCGGTGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                  >  1:415251/1‑70 (MQ=255)
                   accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACGTATCTAAATCCAGAGCATTATgatggg                                                  >  1:3721280/1‑70 (MQ=255)
                   accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                  >  1:3398683/1‑70 (MQ=255)
                   accaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                  >  1:829866/1‑70 (MQ=255)
                   accaGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATggtgggt                                                 >  1:5677978/1‑71 (MQ=255)
                    ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatgggt                                                 >  1:1133008/1‑70 (MQ=255)
                    ccaGACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATgatggg                                                  >  1:4618509/1‑69 (MQ=255)
                     caGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGcgcg                                                            <  1:1430205/58‑1 (MQ=255)
                       gACGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAAt                                              >  1:3131719/1‑70 (MQ=255)
                       gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                             <  1:3421319/71‑1 (MQ=255)
                       gACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                             <  1:585195/71‑1 (MQ=255)
                        aCGTTTCAGATACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGtaataa                                            <  1:1714656/71‑1 (MQ=255)
                          gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                          <  1:4374755/71‑1 (MQ=255)
                          gCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAAc                                          >  1:5113055/1‑71 (MQ=255)
                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                        <  1:5619305/69‑1 (MQ=255)
                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                        <  1:4481914/69‑1 (MQ=255)
                              gAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACgg                                        <  1:743625/69‑1 (MQ=255)
                               agatACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcg                                     <  1:4996120/68‑1 (MQ=255)
                                 gTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTAt                                                        <  1:752053/50‑1 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCATATACATATCTAAATCCAGAGCATTAt                                                        <  1:280149/49‑1 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTAt                                                        <  1:4654202/49‑1 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                   >  1:5531915/2‑69 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                   >  1:4868777/2‑69 (MQ=255)
                                 atACAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGcgag                                   >  1:2999489/2‑69 (MQ=255)
                                  tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                   >  1:5297841/1‑70 (MQ=255)
                                  tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                   >  1:1524639/1‑70 (MQ=255)
                                  tACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                  <  1:3213693/71‑1 (MQ=255)
                                   aCAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                   >  1:4957127/1‑69 (MQ=255)
                                    cAGTTCCGGTGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCa                                 <  1:2054571/70‑1 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                 <  1:1493471/38‑1 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                 <  1:5547392/38‑1 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                >  1:730371/1‑71 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                <  1:2744063/71‑1 (MQ=255)
                                    cAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAg                                <  1:4187216/71‑1 (MQ=255)
                                    cAGCTCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                 <  1:1520136/70‑1 (MQ=255)
                                      gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                 <  1:5223067/36‑1 (MQ=255)
                                      gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCa                                                                 <  1:2269693/36‑1 (MQ=255)
                                      gTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCg                              >  1:2763614/1‑71 (MQ=255)
                                       ctCCGGCGCGATACGCAGATACATATCTAAATCCAGAGCATTATGATGGGTAATAAACGGGCGAGCa                                 <  1:2434266/66‑1 (MQ=255)
                                        tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                             >  1:1682187/1‑70 (MQ=255)
                                        tCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCgg                             >  1:3631966/1‑70 (MQ=255)
                                         ccGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                            <  1:3232740/70‑1 (MQ=255)
                                          cGGCGCGATTCGCAGGTACATGTCGAGATCCAGCGCGt                                                           <  1:1514816/38‑1 (MQ=255)
                                          cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGt                                                           <  1:4096482/38‑1 (MQ=255)
                                          cGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCAc                          >  1:37593/1‑71 (MQ=255)
                                            gcgcGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAc                                      <  1:2283399/57‑1 (MQ=255)
                                             cgcgATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGCTAAACGGACGCGCAGCGGCACCGc                       <  1:5365137/71‑1 (MQ=255)
                                                gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                    <  1:1173550/71‑1 (MQ=255)
                                                gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                    <  1:1766010/71‑1 (MQ=255)
                                                gATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAg                    <  1:1631914/71‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATa                                             <  1:1623944/45‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                         <  1:2549212/49‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACg                                         <  1:886589/49‑1 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                  >  1:2779726/1‑56 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                   >  1:3536593/1‑71 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                   >  1:4510568/1‑71 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                   >  1:4463565/1‑71 (MQ=255)
                                                 aTACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAgg                   >  1:2634568/1‑71 (MQ=255)
                                                  tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGa                                               >  1:5393793/1‑42 (MQ=255)
                                                  tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                      <  1:3841223/67‑1 (MQ=255)
                                                  tACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAggg                  <  1:182418/71‑1 (MQ=255)
                                                    cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCa                     >  1:2929089/1‑66 (MQ=255)
                                                    cGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGa                 <  1:3943651/70‑1 (MQ=255)
                                                     gCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCa                           <  1:211022/59‑1 (MQ=255)
                                                      cAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCa              <  1:2229378/71‑1 (MQ=255)
                                                       aGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCAc             <  1:1902217/71‑1 (MQ=255)
                                                         gTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt           <  1:2411358/71‑1 (MQ=255)
                                                          tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACgcgc                                  <  1:4856365/47‑1 (MQ=255)
                                                          tACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTg          >  1:3909127/1‑71 (MQ=255)
                                                             aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcat       >  1:3289528/1‑71 (MQ=255)
                                                             aTGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGca        >  1:5083439/1‑70 (MQ=255)
                                                              tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGAcgcg                                   <  1:1338097/42‑1 (MQ=255)
                                                              tGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatc      <  1:2953586/71‑1 (MQ=255)
                                                                tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGcc                      <  1:2705047/53‑1 (MQ=255)
                                                                tCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGcatca     <  1:3846328/70‑1 (MQ=255)
                                                                  gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCt           >  1:2118789/1‑62 (MQ=255)
                                                                  gagaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCg  >  1:1910079/1‑71 (MQ=255)
                                                                   agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                            >  1:5679920/1‑44 (MQ=255)
                                                                   agaTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGc                            >  1:755035/1‑44 (MQ=255)
                                                                    |                                                                    
ACGCTCGAAGCCACCAACCACCAGACGCTTGAGGTACAGTTCCGGCGCGATACGCAGGTACATGTCGAGATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCG  >  minE/1933681‑1933817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: