| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,457 | A→G | 19.2% | I472I (ATT→ATC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,457 | 0 | A | G | 19.2% | 299.3 / 53.0 | 125 | I472I (ATT→ATC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (43/58); new base G (9/15); total (52/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.18e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.39e-01 | |||||||||||
Only 100 of 126 total aligned reads displayed.
GGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGA > minE/311388‑311525 | ggtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTaa > 1:1744286/1‑71 (MQ=255) gtggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAAt < 1:1732544/71‑1 (MQ=255) tggCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa < 1:1762660/71‑1 (MQ=255) gCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa > 1:4996799/1‑69 (MQ=255) aaGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAACGCCATTGCTGAAACAATGGTAATAGAGAACt > 1:3903711/1‑71 (MQ=255) agagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTg < 1:4718153/71‑1 (MQ=255) gagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGa < 1:4798511/71‑1 (MQ=255) gagCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGa < 1:5594981/71‑1 (MQ=255) agCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:156881/71‑1 (MQ=255) gCTGGAGTCAGGATCAACGCCACCAGTACCTACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:303446/70‑1 (MQ=255) gCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:4281210/70‑1 (MQ=255) cTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc > 1:2590486/1‑69 (MQ=255) ggAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga > 1:1241653/1‑69 (MQ=255) ggAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga > 1:4453816/1‑69 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACt < 1:688034/63‑1 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:4413214/70‑1 (MQ=255) gAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgata < 1:1889252/70‑1 (MQ=255) aGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACga > 1:1595649/1‑67 (MQ=255) tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa < 1:302890/53‑1 (MQ=255) tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa < 1:5185310/53‑1 (MQ=255) tCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATa < 1:4649763/53‑1 (MQ=255) tCAGGATCAACGCCACCAGTACAGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatagata < 1:4491167/71‑1 (MQ=255) agaaTCAATGCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGc < 1:4657457/68‑1 (MQ=9) aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:3808007/61‑1 (MQ=255) aGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGatagatag > 1:2271140/1‑70 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCa > 1:5493319/1‑70 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:5080207/1‑71 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:5538500/1‑71 (MQ=255) gATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:2687123/1‑71 (MQ=255) aTCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGAc < 1:1375858/58‑1 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:1338692/1‑63 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:2619024/1‑63 (MQ=255) tCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCAc > 1:3183534/1‑69 (MQ=255) cAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTATTAGAGAACTGACGATAGATAGCACCAg > 1:3482320/1‑71 (MQ=255) aatgCCACCAGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGt < 1:2056951/68‑1 (MQ=9) aaCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAg < 1:483869/70‑1 (MQ=255) aCGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGaa > 1:2156626/1‑49 (MQ=255) cGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGaa > 1:2794765/1‑48 (MQ=255) gCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGa < 1:228216/71‑1 (MQ=255) ccaccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa > 1:1947621/1‑71 (MQ=255) ccaccaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgataaat < 1:5341182/58‑1 (MQ=9) ccaccaGAACAGAAAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgataaat < 1:1538083/58‑1 (MQ=9) caccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa < 1:1981799/70‑1 (MQ=255) caccaGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGaa < 1:3828326/70‑1 (MQ=255) cagaacagaaAGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCg < 1:3048365/70‑1 (MQ=12) aGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGc > 1:1819492/1‑70 (MQ=255) gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:93049/71‑1 (MQ=255) gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:1905006/71‑1 (MQ=255) tACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgataga > 1:3704843/1‑50 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:1057858/60‑1 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:3573257/70‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACgatag < 1:2221125/46‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:669992/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:1989027/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:2455357/71‑1 (MQ=255) gaaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCTATAAATTGCCCCAGTAGAACCGCCgaagaa < 1:3210604/68‑1 (MQ=12) gaaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaa < 1:2556783/68‑1 (MQ=12) gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag > 1:1456554/1‑69 (MQ=255) aaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCgata < 1:4078824/41‑1 (MQ=14) aCAGCGCCATTGCTGAAACAATGGTAATGGAGAACTGACGATAGATAGCAcc < 1:377635/52‑1 (MQ=255) aCAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaag > 1:3356927/1‑71 (MQ=255) cAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAgg > 1:96034/1‑71 (MQ=255) aGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGc > 1:454850/1‑71 (MQ=12) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:4182131/1‑66 (MQ=255) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:9675/1‑66 (MQ=255) cgcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGccc > 1:1488991/1‑45 (MQ=2) cgcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGccc > 1:5450556/1‑45 (MQ=2) cgcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGccc > 1:3311290/1‑45 (MQ=2) cgcCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGcca > 1:4314905/1‑45 (MQ=2) cATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCa > 1:2871521/1‑46 (MQ=255) cATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGccg < 1:1130810/58‑2 (MQ=12) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:4161754/71‑1 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:5331884/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:3313267/58‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:486730/1‑70 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:3403910/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:64888/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:5332686/71‑1 (MQ=255) tGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCgga > 1:1026478/1‑69 (MQ=9) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:5141432/46‑1 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAg < 1:260148/46‑1 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:2425457/1‑71 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:5204418/1‑71 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:5294934/1‑71 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:1691408/1‑71 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc < 1:4707146/70‑1 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:4232480/71‑1 (MQ=255) gAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:4973337/1‑71 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa < 1:2727620/55‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:744121/68‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:4185394/68‑1 (MQ=255) aCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:2289126/1‑62 (MQ=255) cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc < 1:45062/71‑1 (MQ=255) aTGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc > 1:4106045/1‑71 (MQ=255) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:115177/71‑2 (MQ=9) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct > 1:4012000/1‑70 (MQ=9) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:4585766/71‑2 (MQ=9) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:4220291/71‑2 (MQ=9) ggTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:2447143/47‑1 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:400823/55‑1 (MQ=255) | GGTGGCACAAAGAGCTGGAGTCAGGATCAACGCCACCAGTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGA > minE/311388‑311525 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |