| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,819 | C→G | 16.2% | T208T (ACG→ACC) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,819 | 0 | C | G | 16.2% | 161.0 / 21.5 | 68 | T208T (ACG→ACC) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (24/33); new base G (9/2); total (33/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.14e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
ATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTT > minE/1933750‑1933889 | aTCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGc > 1:218407/1‑70 (MQ=255) aGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc < 1:299525/71‑1 (MQ=255) gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTc > 1:1235064/1‑70 (MQ=255) gcgcGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCa < 1:2873253/71‑1 (MQ=255) gcgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCaa < 1:4238244/70‑1 (MQ=255) cgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt < 1:4157284/71‑1 (MQ=255) cgTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACt > 1:3994413/1‑71 (MQ=255) gTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACtt > 1:4633813/1‑71 (MQ=255) ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc > 1:4341644/1‑71 (MQ=255) ttATGATGGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTc > 1:1266663/1‑71 (MQ=255) tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc > 1:2900545/1‑70 (MQ=255) tATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTc > 1:2907183/1‑70 (MQ=255) atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa > 1:1629431/1‑71 (MQ=255) atgatgGGTAATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCa > 1:5466831/1‑71 (MQ=255) aTGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa > 1:2968749/1‑71 (MQ=255) tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCa < 1:768147/70‑1 (MQ=255) tggtggGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCAt < 1:5063368/71‑1 (MQ=255) gatgggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTACCAt > 1:5275157/3‑70 (MQ=255) gtgggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATaa < 1:2920968/71‑1 (MQ=255) ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc < 1:1764973/71‑1 (MQ=255) ggtgATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGc < 1:2354082/71‑1 (MQ=255) ggtaATAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAg < 1:1906686/70‑1 (MQ=255) gtaaTAAACGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGcc < 1:4942852/68‑1 (MQ=255) gATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgc > 1:3084545/1‑71 (MQ=255) aTAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgcg > 1:1694715/1‑71 (MQ=255) tAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCgg < 1:4098648/71‑1 (MQ=255) aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATaaa > 1:810487/1‑63 (MQ=12) aaaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgc > 1:980354/1‑69 (MQ=255) aaCGGGCGAGCAGATGCCCCACCTGGAATTACCTGCATCATCGGGGTTTCTACTTCCATAAAGCcgcgcgc > 1:2829337/1‑68 (MQ=255) ggACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcg < 1:1974073/63‑1 (MQ=255) ggACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAc < 1:3778846/71‑1 (MQ=255) gACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCAc > 1:5549767/1‑70 (MQ=255) gcgcAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATg < 1:832179/71‑1 (MQ=255) cgcAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGttt < 1:1535552/41‑1 (MQ=255) aGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACt < 1:910343/71‑1 (MQ=255) gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg < 1:5306629/71‑1 (MQ=255) gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg < 1:4079909/71‑1 (MQ=255) gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg < 1:1211218/71‑1 (MQ=255) gCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg < 1:1625086/71‑1 (MQ=255) aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCAt < 1:4141221/60‑1 (MQ=255) aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa > 1:4014552/1‑71 (MQ=255) aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa > 1:4413358/1‑71 (MQ=255) aCCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGaa > 1:3102022/1‑71 (MQ=255) ccGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCAGCGGTTCACCATg < 1:4670574/60‑1 (MQ=255) gCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATAc > 1:5546473/1‑71 (MQ=255) cAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCa < 1:1764683/71‑1 (MQ=255) gggATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCaga < 1:452627/71‑1 (MQ=255) ggATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg < 1:4245741/61‑1 (MQ=255) gATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCa > 1:369778/1‑47 (MQ=255) gATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCaga > 1:3739751/1‑69 (MQ=255) aTCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCcgcg > 1:1619219/1‑41 (MQ=255) tCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCagagag > 1:5295375/1‑70 (MQ=255) aCCTGCATCATCGGGGTTTCTACTTCCATAAAGCCGCGCGCGACCATGAATTGACgga > 1:5677450/1‑56 (MQ=16) cTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGa < 1:5352279/47‑1 (MQ=255) cTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCg < 1:2535973/54‑1 (MQ=255) cTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCt < 1:810334/71‑1 (MQ=255) tGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGAt > 1:1117647/1‑68 (MQ=255) gCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAAt < 1:2922749/55‑1 (MQ=255) gCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCagaga > 1:4572828/1‑63 (MQ=255) gCATCATCGGCGTTTCAACTTCCATAAAGCCGCCGTTCAcc > 1:944456/1‑41 (MQ=255) catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGc < 1:5341534/70‑1 (MQ=255) catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGc < 1:2209908/70‑1 (MQ=255) catcatCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGc < 1:4214645/70‑1 (MQ=255) atcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGc > 1:5338288/1‑46 (MQ=255) atcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAgcg < 1:2856231/71‑1 (MQ=255) tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGa > 1:2591118/1‑47 (MQ=255) tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGa > 1:4252413/1‑47 (MQ=255) tcGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAgcg > 1:4508284/1‑70 (MQ=255) cGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACt < 1:3181212/41‑1 (MQ=255) cGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACtt < 1:5378127/71‑1 (MQ=255) | ATCCAGCGCGTTATGGTGGGTGATAAACGGACGCGCAGCGGCACCGCCAGGGATCACCTGCATCATCGGCGTTTCAACTTCCATAAAGCCGCGGTTCACCATGAACTGGCGAATACCAGAGAGGATCTGCGAGCGCACTT > minE/1933750‑1933889 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |