| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,945 | G→A | 26.6% | R166R (CGC→CGT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,945 | 0 | G | A | 26.6% | 128.6 / 49.4 | 83 | R166R (CGC→CGT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (23/37); new base A (11/11); total (35/48) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.49e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
TCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGC > minE/1933875‑1934014 | tCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAg > 1:651522/1‑71 (MQ=255)tCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATAGAGATAACGCTGACGATAg > 1:2970932/1‑71 (MQ=255) gCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAgcg > 1:607602/1‑70 (MQ=255) ttAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATc > 1:2134591/1‑71 (MQ=255) aaGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTg < 1:2590196/71‑1 (MQ=255) aGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGc < 1:1453479/71‑1 (MQ=255) ggTGTTGCGGGATTCATCGTTGGAGATTAGATCGAGATAACGCTGACGATAGCGCGCTTcc < 1:4083618/61‑1 (MQ=255) ggTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTcc < 1:785053/61‑1 (MQ=255) ggTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCa < 1:2689667/71‑1 (MQ=255) gtgtTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCa < 1:24950/70‑1 (MQ=255) tgtTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTg > 1:749180/1‑61 (MQ=255) ttGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGc < 1:2095220/70‑1 (MQ=255) ttGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGc < 1:4792362/70‑1 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt > 1:3136849/1‑71 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt > 1:1526006/1‑71 (MQ=255) gCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt > 1:1441388/1‑71 (MQ=255) gCGGGATTCATCGTT‑GAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTg > 1:5055691/1‑71 (MQ=255) gggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg < 1:4292694/71‑1 (MQ=255) gggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg < 1:2935649/71‑1 (MQ=255) gggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgg < 1:397563/71‑1 (MQ=255) ggATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGa < 1:94396/71‑1 (MQ=255) ggATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:5435180/71‑1 (MQ=255) gATTTATCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTg > 1:2460759/1‑59 (MQ=255) taTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt > 1:5341320/2‑70 (MQ=255) taTCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt < 1:1652139/69‑1 (MQ=255) cATCGTAGGAGATGACATCGAGATAACGCTGACGATAGCGCGCTTcc > 1:3249342/1‑47 (MQ=38) aTCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt < 1:2347465/71‑1 (MQ=255) tCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATc > 1:5022455/1‑71 (MQ=255) tCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATc < 1:5118446/71‑1 (MQ=255) tCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATc > 1:1704494/1‑71 (MQ=255) tCGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATc < 1:962843/71‑1 (MQ=255) cGTTAGCGATGAGGTCCAGATAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATc < 1:4640566/70‑1 (MQ=255) gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCg > 1:2002469/1‑71 (MQ=255) gTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCg > 1:4588203/1‑71 (MQ=255) ttGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg < 1:3679940/71‑1 (MQ=255) ggAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCgg > 1:2110155/1‑69 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:3141770/71‑1 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:468999/71‑1 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:4376784/71‑1 (MQ=255) gatgagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCg < 1:1631048/71‑1 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:4377217/1‑70 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGa > 1:3111569/1‑70 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc > 1:2724307/1‑71 (MQ=255) gagatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc > 1:2646964/1‑71 (MQ=255) agatCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGAc < 1:570914/70‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTg > 1:1639718/1‑48 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCa > 1:1219607/1‑62 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCa < 1:605297/62‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCa < 1:2942886/62‑1 (MQ=255) atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGc > 1:3726896/1‑70 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGt > 1:5324149/1‑45 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:462623/1‑71 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:5245815/1‑71 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:2749457/1‑71 (MQ=255) cGAGATAACGCTGACGAT‑‑CGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGc > 1:421839/1‑71 (MQ=255) agaTAACGCTGTCGATAGCGCGCTTCCTGATCCTGCaa < 1:136216/38‑1 (MQ=255) agaTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGc < 1:2135753/71‑1 (MQ=255) aTAACGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACgt < 1:5205120/64‑2 (MQ=255) aTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTgta < 1:1407849/44‑3 (MQ=255) aTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:892463/44‑1 (MQ=255) tAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:4025334/43‑1 (MQ=255) tAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTAt < 1:1386521/49‑1 (MQ=255) aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGaa > 1:22073/1‑43 (MQ=255) aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt > 1:4850361/1‑69 (MQ=255) aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt > 1:3160493/1‑69 (MQ=255) aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtttag < 1:3315533/71‑3 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGcc > 1:1559433/1‑36 (MQ=255) aCGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATcc > 1:2591524/1‑49 (MQ=255) gttGACGATAACGGACTTCCTGATCCTGCAGACCATGGGATTTATCTGGTAAAGGACGTAGTGCTTTAGTc < 1:880468/69‑1 (MQ=255) gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCtt < 1:3626070/66‑1 (MQ=255) ttGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta < 1:4037612/66‑2 (MQ=255) cTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCtt < 1:4503008/65‑1 (MQ=255) tGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:1782687/37‑1 (MQ=255) tGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGa < 1:3266450/37‑1 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt > 1:4853913/1‑40 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAAttt > 1:243451/1‑40 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag > 1:1287451/1‑70 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag < 1:408624/70‑1 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag > 1:460533/1‑70 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCgg < 1:3369224/51‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCgg < 1:2177350/51‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagca < 1:871873/71‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagca < 1:5361150/71‑1 (MQ=255) aCGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcagc < 1:4004532/70‑1 (MQ=255) aTAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACg < 1:3778771/51‑1 (MQ=255) aTAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGc < 1:4950430/57‑1 (MQ=255) gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:3250009/70‑1 (MQ=255) | TCTGCGAGCGCACTTTAAAGGTGTTGCGGGATTCATCGTTGGAGATGAGATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGC > minE/1933875‑1934014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |