| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,933,993 | C→T | 42.5% | L150L (CTG→CTA) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,933,993 | 0 | C | T | 42.5% | 39.7 / 96.0 | 87 | L150L (CTG→CTA) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (17/33); new base T (16/21); total (33/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.03e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.92e-01 | |||||||||||
ATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAAC > minE/1933924‑1934059 | atCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGc > 1:3726896/1‑70 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:462623/1‑71 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:2749457/1‑71 (MQ=255) cGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGt > 1:5245815/1‑71 (MQ=255) cGAGATAACGCTGACGAT‑‑CGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGc > 1:421839/1‑71 (MQ=255) agaTAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGc < 1:2135753/71‑1 (MQ=255) aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt > 1:3160493/1‑69 (MQ=255) aaCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtt > 1:4850361/1‑69 (MQ=255) aCGTTGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCtttag < 1:3315533/71‑3 (MQ=255) gttGACGATAACGGACTTCCTGATCCTGCAGACCATGGGATTTATCTGGTAAAGGACGTAGTGCTTTAGTc < 1:880468/69‑1 (MQ=255) gCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCtt < 1:3626070/66‑1 (MQ=255) ttGACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta < 1:4037612/66‑2 (MQ=255) cTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCtt < 1:4503008/65‑1 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag < 1:408624/70‑1 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag > 1:460533/1‑70 (MQ=255) gACGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag > 1:1287451/1‑70 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagca < 1:5361150/71‑1 (MQ=255) aCGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTcagca < 1:871873/71‑1 (MQ=255) aCGATAACGGACTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcagc < 1:4004532/70‑1 (MQ=255) aTAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGc < 1:4950430/57‑1 (MQ=255) gcgcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGc < 1:3250009/70‑1 (MQ=255) gcgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAAc > 1:3602980/1‑71 (MQ=255) gacTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt < 1:3181762/69‑1 (MQ=255) gacTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCt < 1:4835933/69‑1 (MQ=255) cgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGt > 1:2058026/1‑57 (MQ=255) cgcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt > 1:1744131/1‑71 (MQ=255) gcTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTc < 1:3085453/71‑1 (MQ=255) acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag < 1:161760/58‑1 (MQ=255) acTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTcag < 1:3137036/58‑1 (MQ=255) cTTCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCACCt > 1:1213546/1‑69 (MQ=255) ttCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcgc < 1:4853049/64‑1 (MQ=255) ttCCTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcgc < 1:3471179/64‑1 (MQ=255) tCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCt < 1:806994/48‑1 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttta > 1:5470677/1‑49 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTcag < 1:1144142/69‑3 (MQ=255) ccTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta > 1:5676856/1‑70 (MQ=255) ccTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGAc > 1:94270/1‑60 (MQ=255) cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTca > 1:4077343/1‑66 (MQ=255) cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGtac < 1:4136137/71‑3 (MQ=255) cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGtac < 1:1974970/71‑3 (MQ=255) cTGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGta < 1:4003635/70‑2 (MQ=255) tGATCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCa > 1:2312386/1‑61 (MQ=255) tGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg > 1:285824/1‑69 (MQ=255) aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt < 1:4825226/71‑1 (MQ=255) aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTgg < 1:2292799/47‑1 (MQ=255) aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGGGCAGt > 1:1274071/1‑71 (MQ=255) aTCCTGCAAGCCGTGGAATTTATACGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt > 1:2917538/1‑71 (MQ=255) tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt > 1:3631352/1‑70 (MQ=255) tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt > 1:4916423/1‑70 (MQ=255) tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt > 1:2325026/1‑70 (MQ=255) tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTg < 1:4174745/71‑1 (MQ=255) tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg < 1:3500613/71‑1 (MQ=255) tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg < 1:1761591/71‑1 (MQ=255) tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGAGCAGTg < 1:735382/71‑1 (MQ=255) cTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCttt < 1:1921866/42‑1 (MQ=255) ccGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg < 1:4668860/71‑1 (MQ=255) catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc > 1:2573148/3‑70 (MQ=255) catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc > 1:4588118/3‑70 (MQ=255) catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc > 1:1855730/3‑70 (MQ=255) cGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa < 1:4822578/69‑1 (MQ=255) atGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTg < 1:1258198/59‑1 (MQ=255) tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGgcgc < 1:3170555/45‑1 (MQ=255) tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTc < 1:3823357/71‑1 (MQ=255) tGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCg < 1:2863152/51‑1 (MQ=255) ggAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGa > 1:2926615/1‑64 (MQ=255) gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGc < 1:191712/71‑1 (MQ=255) aaTTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTc < 1:386645/47‑1 (MQ=255) tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt < 1:2585115/44‑1 (MQ=255) tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt < 1:3768983/44‑1 (MQ=255) tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACt < 1:5386285/44‑1 (MQ=255) tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTgg < 1:3767021/55‑1 (MQ=255) tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCgg > 1:2880493/1‑71 (MQ=255) ttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc < 1:2890008/60‑1 (MQ=255) tATCCCGCAGCGGACGCAGTGCTTTGGTCAGCAGACg > 1:4659781/1‑37 (MQ=255) cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg < 1:3564349/71‑1 (MQ=255) cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg < 1:5295937/71‑1 (MQ=255) gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTc < 1:4178431/58‑1 (MQ=255) gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt > 1:5415451/1‑71 (MQ=255) gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt > 1:1499819/1‑71 (MQ=255) aGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt > 1:4613305/1‑65 (MQ=255) gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa < 1:1941728/49‑1 (MQ=255) gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa < 1:4663411/49‑1 (MQ=255) gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa < 1:861621/49‑1 (MQ=255) gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg < 1:215619/71‑1 (MQ=255) gCGGACGCAGTGCTTTGGGCAGCAGACGCAACTCGGTGCAGTGGATAGa < 1:3394994/49‑1 (MQ=255) cGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGa < 1:5582937/71‑1 (MQ=255) gACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAAc < 1:4641451/71‑1 (MQ=255) aCGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGaa > 1:2462441/1‑69 (MQ=255) cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg < 1:4849127/45‑1 (MQ=255) cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg < 1:1403694/45‑1 (MQ=255) cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg < 1:3005811/45‑1 (MQ=255) cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg < 1:3062072/45‑1 (MQ=255) | ATCGAGATAACGCTGACGATAGCGCGCTTCCTGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAAC > minE/1933924‑1934059 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |