Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,934,023 G→A 14.3% C140C (TGC→TGT lysS ← lysine tRNA synthetase, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,934,0230GA14.3% 186.1 / 19.1 77C140C (TGC→TGTlysSlysine tRNA synthetase, constitutive
Reads supporting (aligned to +/- strand):  ref base G (27/39);  new base A (7/4);  total (34/43)
Fisher's exact test for biased strand distribution p-value = 1.99e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.97e-01

TGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGT  >  minE/1933955‑1934093
                                                                    |                                                                      
tGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTg                                                                        >  1:285824/1‑69 (MQ=255)
  aTCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                                                    <  1:4825226/71‑1 (MQ=255)
  aTCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGGGCAGt                                                                    >  1:1274071/1‑71 (MQ=255)
  aTCCTGCAAGCCGTGGAATTTATACGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGt                                                                    >  1:2917538/1‑71 (MQ=255)
   tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                                                    >  1:2325026/1‑70 (MQ=255)
   tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                                                    >  1:4916423/1‑70 (MQ=255)
   tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGt                                                                    >  1:3631352/1‑70 (MQ=255)
   tCCTGCAGACCATGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTg                                                                   <  1:4174745/71‑1 (MQ=255)
   tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg                                                                   <  1:1761591/71‑1 (MQ=255)
   tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTg                                                                   <  1:3500613/71‑1 (MQ=255)
   tCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGAGCAGTg                                                                   <  1:735382/71‑1 (MQ=255)
            ccGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                                          <  1:4668860/71‑1 (MQ=255)
             catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc                                                         >  1:4588118/3‑70 (MQ=255)
             catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc                                                         >  1:2573148/3‑70 (MQ=255)
             catGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAgc                                                         >  1:1855730/3‑70 (MQ=255)
             cGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACa                                                           <  1:4822578/69‑1 (MQ=255)
              atGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTg                                                                   <  1:1258198/59‑1 (MQ=255)
               tGGAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGAAAGCTc                                                       <  1:3823357/71‑1 (MQ=255)
                ggAATTTATCTGGTAAAGGACGTAGTGCTTTAGTCAGCAGGCGCAGCTCAGTACAGTGAATGGa                                                             >  1:2926615/1‑64 (MQ=255)
                 gAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGc                                                     <  1:191712/71‑1 (MQ=255)
                    tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTgg                                                                  <  1:3767021/55‑1 (MQ=255)
                    tttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCgg                                                  >  1:2880493/1‑71 (MQ=255)
                     ttATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc                                                            <  1:2890008/60‑1 (MQ=255)
                          cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg                                            <  1:3564349/71‑1 (MQ=255)
                          cGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttgg                                            <  1:5295937/71‑1 (MQ=255)
                            gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTc                                                       <  1:4178431/58‑1 (MQ=255)
                            gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt                                          >  1:5415451/1‑71 (MQ=255)
                            gCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttttggtt                                          >  1:1499819/1‑71 (MQ=255)
                              aGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtttt                                              >  1:4613305/1‑65 (MQ=255)
                               gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                                             <  1:861621/49‑1 (MQ=255)
                               gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                                             <  1:1941728/49‑1 (MQ=255)
                               gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                                             <  1:4663411/49‑1 (MQ=255)
                               gCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg                                       <  1:215619/71‑1 (MQ=255)
                               gCGGACGCAGTGCTTTGGGCAGCAGACGCAACTCGGTGCAGTGGATAGa                                                             <  1:3394994/49‑1 (MQ=255)
                                cGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGa                                      <  1:5582937/71‑1 (MQ=255)
                                  gACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAAc                                    <  1:4641451/71‑1 (MQ=255)
                                   aCGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGaa                                     >  1:2462441/1‑69 (MQ=255)
                                      cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                                          <  1:1403694/45‑1 (MQ=255)
                                      cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                                          <  1:4849127/45‑1 (MQ=255)
                                      cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                                          <  1:3062072/45‑1 (MQ=255)
                                      cAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                                          <  1:3005811/45‑1 (MQ=255)
                                        gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGAc                                                            <  1:3277571/41‑1 (MQ=255)
                                        gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGt                                                         <  1:5187435/44‑1 (MQ=255)
                                        gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGt                                                         <  1:5414204/44‑1 (MQ=255)
                                        gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGc                                 <  1:1988341/68‑1 (MQ=255)
                                        gTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGc                                 <  1:5055446/68‑1 (MQ=255)
                                         tGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggtt                                                <  1:2294863/52‑1 (MQ=255)
                                          gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc                            >  1:1074442/1‑71 (MQ=255)
                                          gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc                            >  1:854649/1‑71 (MQ=255)
                                          gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc                            >  1:4893703/1‑71 (MQ=255)
                                          gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc                            >  1:4105097/1‑71 (MQ=255)
                                          gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAcc                            >  1:47924/1‑71 (MQ=255)
                                          gCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTAc                             >  1:1799409/1‑70 (MQ=255)
                                           cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                                          >  1:1796875/1‑40 (MQ=255)
                                           cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAg                                                          >  1:5202966/1‑40 (MQ=255)
                                           cTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCt                           >  1:5323365/1‑71 (MQ=255)
                                             ttGGTCAGCAGACGCAACTCGGTGCAGTGGATAGa                                                             <  1:2630518/35‑1 (MQ=255)
                                              tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACa                                   >  1:591886/1‑60 (MQ=255)
                                              tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCttt                         <  1:2978676/70‑1 (MQ=255)
                                              tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCtt                          <  1:4657059/69‑1 (MQ=255)
                                              tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTc                        >  1:5371789/1‑71 (MQ=255)
                                              tGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTc                        >  1:2457260/1‑71 (MQ=255)
                                               ggTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTcg                       >  1:1529964/1‑71 (MQ=255)
                                                   agcagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGcc                   >  1:5550570/1‑71 (MQ=255)
                                                   agcagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGcc                   <  1:5575288/71‑1 (MQ=255)
                                                     cagTCGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg                                       <  1:848158/49‑1 (MQ=255)
                                                     cagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGgttttggttttg                                       <  1:969624/49‑1 (MQ=255)
                                                     cagACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCtt                               <  1:4008047/57‑1 (MQ=255)
                                                      agACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAg                >  1:5254303/1‑71 (MQ=255)
                                                      agACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAg                <  1:4847325/71‑1 (MQ=255)
                                                      agACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAg                >  1:4204476/1‑71 (MQ=255)
                                                      agACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAg                >  1:3497594/1‑71 (MQ=255)
                                                       gACGCAACTCGGTGCAGTGGATAGACAGTTCGCCggttt                                               <  1:3367423/39‑1 (MQ=255)
                                                       gACGCAACTCGGTGCAGTGGATAGACAGTTAGCCggttt                                               <  1:4013623/39‑1 (MQ=255)
                                                          gCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGa              >  1:1397935/1‑69 (MQ=255)
                                                               tCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGcc       >  1:4870298/1‑71 (MQ=255)
                                                                cGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAAc                                    >  1:2572662/1‑41 (MQ=255)
                                                                  gTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAg    <  1:2246046/71‑1 (MQ=255)
                                                                    gCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGcc       <  1:4298036/66‑1 (MQ=255)
                                                                    gCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGt  >  1:2464414/1‑71 (MQ=255)
                                                                    |                                                                      
TGATCCTGCAAGCCGTGGAATTTATCCGGCAGCGGACGCAGTGCTTTGGTCAGCAGACGCAACTCGGTGCAGTGGATAGACAGTTCGCCGGTTTTGGTTTTGAACAGCTTACCTTTCGCGCCGAGGATGTCGCCGAGGT  >  minE/1933955‑1934093

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: