| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,167 | G→A | 22.5% | G92G (GGC→GGT) | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,167 | 0 | G | A | 22.5% | 189.4 / 51.3 | 102 | G92G (GGC→GGT) | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base G (37/42); new base A (19/4); total (56/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
ATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAA > minE/1934097‑1934234 | aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:2809910/1‑71 (MQ=255)aTTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCg > 1:2943895/1‑71 (MQ=255) tttttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGc > 1:418436/1‑70 (MQ=255) tttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:530027/1‑71 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:3316314/1‑70 (MQ=255) ttttAAACTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCAcc > 1:3243048/1‑70 (MQ=255) tttGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCa < 1:2318093/70‑1 (MQ=255) taaaCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGAca > 1:3441751/3‑70 (MQ=255) taaaCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGAca > 1:2601234/3‑70 (MQ=255) gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg < 1:50296/70‑1 (MQ=255) gAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACg < 1:1604905/70‑1 (MQ=255) aaCTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTc < 1:2636232/71‑1 (MQ=255) aCTGATCGTTATAAACACCTTCTGGCAGGCTATCTCTTGCAACGTACAGTTGAATACGGCCACCGACATcc > 1:3362005/1‑71 (MQ=255) cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTg < 1:2561669/69‑1 (MQ=255) cTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCa < 1:3604473/71‑1 (MQ=255) cGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:4000042/1‑71 (MQ=255) gTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg < 1:1469185/71‑1 (MQ=255) gTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAggg > 1:3317548/1‑71 (MQ=255) tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGc < 1:4041612/65‑1 (MQ=255) tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:218029/1‑70 (MQ=255) tataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGt > 1:4648808/1‑70 (MQ=255) ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:4515001/1‑71 (MQ=255) ataAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTaa > 1:3361187/1‑71 (MQ=255) aaCGCCTTCCGGGAGATCGTCACGGGCAACGTCCAGCTGAATGCGACCGCCa > 1:1493144/1‑52 (MQ=255) cGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTc > 1:1558446/1‑55 (MQ=255) ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGcc < 1:1479649/45‑1 (MQ=255) ttCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGc < 1:3751635/55‑1 (MQ=255) tCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGc > 1:2419192/1‑71 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTTCAGGGTAACGAAAGACGc < 1:4037873/70‑1 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:3594207/1‑71 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:43532/1‑71 (MQ=255) ccGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCt > 1:830366/1‑71 (MQ=255) gggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCttt < 1:2600185/71‑1 (MQ=255) ggAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTa < 1:4818060/71‑1 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGcc > 1:3085563/1‑37 (MQ=255) gaTCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAAc > 1:5595980/1‑40 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1369846/71‑1 (MQ=255) tCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3923450/71‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataat < 1:4953092/71‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:4254397/68‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1500069/68‑1 (MQ=255) tCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:1506555/68‑1 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:2967745/1‑44 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAgg > 1:5028445/1‑44 (MQ=255) cACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGa > 1:3729867/1‑55 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5192515/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:4089260/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5635555/1‑71 (MQ=255) aCGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:2305793/1‑71 (MQ=255) aCGGGCAACGTACAGATGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa > 1:2103692/1‑68 (MQ=255) cttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATac > 1:4010170/4‑70 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5608964/49‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:1083991/49‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:3553945/49‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCat < 1:3261681/65‑1 (MQ=255) cGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATac > 1:5431537/1‑70 (MQ=255) ttgCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATacga > 1:1882957/3‑71 (MQ=255) gCAACGTACAGTTGAATCCGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2586352/1‑59 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:3486407/1‑59 (MQ=255) gCAACGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTccc > 1:2435601/1‑59 (MQ=255) cAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACgg > 1:1178934/1‑71 (MQ=255) aaCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:1086633/59‑1 (MQ=255) aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:1821742/1‑71 (MQ=255) aCGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:3189115/1‑71 (MQ=255) aCGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGt < 1:1442921/71‑1 (MQ=255) cGTACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGt > 1:2923742/1‑70 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:4147612/43‑1 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGaaa < 1:2922051/43‑1 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGa < 1:5027833/41‑1 (MQ=255) gTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtca < 1:2699140/71‑1 (MQ=255) tACAGTTGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGtcat < 1:459105/71‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACg < 1:3633276/46‑1 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat > 1:2695632/1‑71 (MQ=255) tACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcat < 1:192176/71‑1 (MQ=255) aCAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacg > 1:1351298/1‑61 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2739834/1‑71 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatca > 1:2369524/1‑71 (MQ=255) cAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGtcatc < 1:5568952/70‑1 (MQ=255) aGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTcatcat < 1:4756993/71‑1 (MQ=255) tGAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:65613/1‑71 (MQ=255) gAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCttt < 1:2181078/43‑1 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2287907/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg > 1:2526588/1‑71 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg > 1:1549295/1‑70 (MQ=255) gAATACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:3245899/70‑1 (MQ=255) aaTGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCa < 1:3626458/47‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCgg < 1:4012404/70‑1 (MQ=255) aaTACGGCCACCGACATCCTGCAACGTTACAAAGGAGGCTTTCCCCATGATACGACGGGTCATCATTCg < 1:4738092/69‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:2251878/48‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCataa < 1:5474961/48‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:5491071/1‑55 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgacg > 1:3691850/1‑55 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:63102/54‑1 (MQ=255) tGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATacgac < 1:759894/54‑1 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:4926516/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:3759086/1‑70 (MQ=255) cGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAg > 1:1058439/1‑70 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:5591560/1‑68 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:4279799/1‑68 (MQ=255) gCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCaa > 1:932022/1‑68 (MQ=255) | ATTTTTTGAACTGCTCGTTATAAACGCCTTCCGGGAGATCGTCACGGGCAACGTACAGCTGAATGCGACCGCCAACGTCCTGCAGGGTAACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAA > minE/1934097‑1934234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |