| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,496 | A→G | 22.6% | F459F (TTT→TTC) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,496 | 0 | A | G | 22.6% | 253.8 / 60.2 | 115 | F459F (TTT→TTC) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base A (44/45); new base G (12/14); total (56/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 125 total aligned reads displayed.
GTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCC > minE/311426‑311566 | gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:93049/71‑1 (MQ=255)gTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCa < 1:1905006/71‑1 (MQ=255) ccGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:3573257/70‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:2455357/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:1989027/71‑1 (MQ=255) cGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:669992/71‑1 (MQ=255) gaaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCTATAAATTGCCCCAGTAGAACCGCCgaagaa < 1:3210604/68‑1 (MQ=12) gaaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaa < 1:3393074/68‑1 (MQ=12) gaaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaa < 1:2556783/68‑1 (MQ=12) gACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaag > 1:1456554/1‑69 (MQ=255) aaaGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCgaagaat > 1:4834798/3‑70 (MQ=12) cAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAgg > 1:96034/1‑71 (MQ=255) aGCGCCATTGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGc > 1:454850/1‑71 (MQ=12) cgcCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa > 1:4182131/1‑66 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:4161754/71‑1 (MQ=255) aTTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:5331884/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCaaa < 1:3313267/58‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:1600655/1‑70 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:486730/1‑70 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:64888/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:3403910/71‑1 (MQ=255) ttGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGt < 1:5332686/71‑1 (MQ=255) tGCCGAAACGATGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCgga > 1:1026478/1‑69 (MQ=9) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:1691408/1‑71 (MQ=255) gCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:5294934/1‑71 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc < 1:4707146/70‑1 (MQ=255) cTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:4232480/71‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaagaa < 1:2727620/55‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:4185394/68‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:771359/68‑1 (MQ=255) aaaCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACg < 1:5077240/68‑1 (MQ=255) aCAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg > 1:2289126/1‑62 (MQ=255) cAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAcc < 1:45062/71‑1 (MQ=255) aTGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATAccgc > 1:4106045/1‑71 (MQ=255) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:4220291/71‑2 (MQ=9) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct > 1:4012000/1‑70 (MQ=9) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:115177/71‑2 (MQ=9) tGGTGATAGAGAACTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:4585766/71‑2 (MQ=9) ggTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAaaga < 1:2447143/47‑1 (MQ=255) gTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa < 1:2636719/71‑1 (MQ=255) tAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCgg < 1:400823/55‑1 (MQ=255) aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGaa > 1:3368717/1‑59 (MQ=255) aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg > 1:4973468/1‑71 (MQ=255) aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg > 1:5367023/1‑71 (MQ=255) tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa < 1:679377/69‑1 (MQ=255) agagAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTa > 1:1350901/1‑71 (MQ=255) gagaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTAc > 1:5282410/1‑53 (MQ=255) agaACTGACGATAGATAGCACCACTAGAACCCCCAaag < 1:2297252/38‑1 (MQ=38) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:1460261/71‑1 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:4286376/71‑1 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:3284158/71‑1 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:2545083/71‑1 (MQ=255) aaCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:3447088/71‑1 (MQ=9) aaCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:950705/71‑1 (MQ=9) aCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:2281518/70‑1 (MQ=9) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa < 1:1229471/48‑1 (MQ=255) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc < 1:567990/71‑1 (MQ=255) cTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg > 1:3512703/1‑71 (MQ=255) gACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt > 1:753224/1‑40 (MQ=255) gACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCAt > 1:4791495/1‑40 (MQ=255) gcGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa < 1:2261693/70‑1 (MQ=12) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:4097488/63‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:2462337/63‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:3048474/1‑71 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:2999704/1‑71 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:3021514/1‑42 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:2907225/1‑42 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTa > 1:2097921/1‑42 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt < 1:5466771/70‑1 (MQ=255) agCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGAc > 1:2910826/1‑69 (MQ=255) gccccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATa > 1:3179630/4‑63 (MQ=12) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:4451474/70‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:1459092/70‑1 (MQ=255) cccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAcc < 1:5367771/51‑1 (MQ=12) cccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAcc < 1:1448029/51‑1 (MQ=12) cAGTAGAACCGCCGAAGAATGCCATCGGAATAAATAccgct < 1:5424972/41‑2 (MQ=9) cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag > 1:3760587/1‑69 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa > 1:4201841/1‑50 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:254290/1‑71 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:5664523/1‑71 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGAAAGTACCATCGCGATACCGACCAGAgcgc > 1:3427005/1‑71 (MQ=255) gTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGcc > 1:5383800/1‑71 (MQ=12) gTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGcc > 1:786918/1‑71 (MQ=255) tAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATc < 1:1082062/50‑1 (MQ=9) tAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATc < 1:3242049/50‑1 (MQ=9) tAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATc < 1:5592764/50‑1 (MQ=9) tAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:2052512/1‑71 (MQ=255) aGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCt > 1:1240559/1‑71 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:780975/1‑71 (MQ=255) gAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa > 1:5134930/1‑52 (MQ=9) gAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa > 1:1058364/1‑52 (MQ=9) gAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa > 1:817718/1‑52 (MQ=9) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:2898020/44‑1 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgcg > 1:1799234/1‑51 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:1090433/1‑70 (MQ=255) aaCCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTg > 1:5073295/1‑70 (MQ=9) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:718203/70‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:260009/70‑1 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCGGACAGTACCATCGCGATACCGACCaga > 1:3391834/1‑58 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc > 1:1816424/1‑71 (MQ=255) | GTACCGACAGCGCCATTGCTGAAACAATGGTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCC > minE/311426‑311566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |