| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,254 | C→G | 17.3% | A63A (GCG→GCC) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,254 | 0 | C | G | 17.3% | 237.3 / 38.8 | 104 | A63A (GCG→GCC) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (39/47); new base G (11/7); total (50/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.01e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
AACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGG > minE/1934185‑1934323 | aaCGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAgcg > 1:4978297/1‑71 (MQ=255) gAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCt > 1:2343918/1‑70 (MQ=255) aaaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc > 1:3316697/1‑71 (MQ=255) aaGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTcc < 1:2878285/71‑1 (MQ=255) aggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt < 1:4023622/69‑1 (MQ=255) ggCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGtt > 1:4066856/2‑71 (MQ=255) gCTTTCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:2613270/1‑71 (MQ=255) tttCCCCATGATACGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcct > 1:3144320/1‑69 (MQ=255) tACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc < 1:2759822/70‑1 (MQ=255) aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:776813/70‑1 (MQ=255) cccATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:4873818/71‑1 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCtt > 1:4280273/1‑56 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct < 1:321230/71‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2739660/55‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2634463/55‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2608632/55‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:1588650/67‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct > 1:2161738/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:386593/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:740975/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:949985/1‑71 (MQ=255) ataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:435370/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:3534875/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt < 1:846781/71‑1 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:2285625/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:5287180/1‑71 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc > 1:666016/1‑70 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc > 1:5462633/1‑70 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCa < 1:5205759/52‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGcc < 1:2983383/71‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc < 1:4796101/70‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc < 1:3307086/70‑1 (MQ=255) gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:3866528/71‑1 (MQ=255) gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa < 1:1442612/71‑1 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt < 1:1445814/50‑1 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt > 1:618336/1‑70 (MQ=255) atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGt < 1:4439579/45‑1 (MQ=255) atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:4256860/1‑59 (MQ=255) atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt < 1:686803/70‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTc < 1:2284141/40‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTc < 1:3931232/64‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:4701572/71‑1 (MQ=255) taatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:5677460/3‑58 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc < 1:4911708/46‑2 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttcc < 1:5380309/46‑2 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:4980481/1‑45 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:4986892/1‑45 (MQ=255) catTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGttc > 1:1633243/1‑45 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa < 1:4416385/65‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg > 1:4585570/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg > 1:5287455/1‑48 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt > 1:5369954/1‑50 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:923928/70‑1 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCCTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:4652666/70‑1 (MQ=255) atGAGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg > 1:4521076/1‑48 (MQ=255) gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:17456/1‑71 (MQ=255) gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:4291881/1‑71 (MQ=255) ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTg < 1:1099486/70‑1 (MQ=255) ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGc < 1:3994730/71‑1 (MQ=255) ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTg < 1:5267452/51‑1 (MQ=255) ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:48892/1‑71 (MQ=255) gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg > 1:714320/1‑71 (MQ=255) cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:3238388/1‑55 (MQ=255) cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:2577827/1‑55 (MQ=255) aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:2595652/71‑1 (MQ=255) aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt > 1:3421109/1‑71 (MQ=255) aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:807830/71‑1 (MQ=255) aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt > 1:3061736/1‑71 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:3417545/70‑1 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTATGCGTGCAATTGGt > 1:1375951/1‑70 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc < 1:2081012/39‑1 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc < 1:5345666/39‑1 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:700856/1‑63 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:3461357/1‑63 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:1073703/1‑63 (MQ=255) gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:4214467/67‑1 (MQ=255) gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa > 1:851014/1‑69 (MQ=255) gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTATTTGCCGTCGAATTCTGCGCGCAATTGGTCa > 1:1746159/1‑69 (MQ=255) gACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAgg > 1:4700983/1‑71 (MQ=255) aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag < 1:5408656/67‑1 (MQ=255) aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag < 1:2590850/67‑1 (MQ=255) aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag < 1:2462445/67‑1 (MQ=255) cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTa > 1:2552620/1‑71 (MQ=255) ttCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa < 1:5508224/44‑1 (MQ=255) ttCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:1077842/49‑1 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTAt < 1:3968678/69‑1 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATg > 1:3166334/1‑70 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:3159777/1‑71 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:2747049/1‑71 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:874971/1‑71 (MQ=255) gATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa < 1:2304240/70‑1 (MQ=255) aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:4991798/58‑1 (MQ=255) aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATc < 1:813340/71‑1 (MQ=255) ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:1739618/55‑1 (MQ=255) ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc < 1:3862576/70‑1 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAGTTCTGCGTGCAATTGGt > 1:759464/1‑53 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc > 1:5045988/1‑71 (MQ=255) aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:3900552/39‑1 (MQ=255) aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc < 1:4588395/70‑1 (MQ=255) cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACgg > 1:527619/1‑70 (MQ=255) | AACGAAAGACGCTTTACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGG > minE/1934185‑1934323 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |