| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,934,266 | T→C | 10.3% | E59E (GAA→GAG) ‡ | lysS ← | lysine tRNA synthetase, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,934,266 | 0 | T | C | 10.3% | 256.8 / 16.1 | 97 | E59E (GAA→GAG) ‡ | lysS | lysine tRNA synthetase, constitutive |
| Reads supporting (aligned to +/- strand): ref base T (43/44); new base C (5/5); total (48/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGG > minE/1934199‑1934334 | tACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttcttc < 1:2759822/70‑1 (MQ=255) aCCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg < 1:776813/70‑1 (MQ=255) cATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtct < 1:321230/71‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGtt < 1:1588650/67‑1 (MQ=255) taataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTctct > 1:2161738/1‑71 (MQ=255) aataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCtt > 1:949985/1‑71 (MQ=255) ataCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:435370/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:3534875/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt < 1:846781/71‑1 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:2285625/1‑71 (MQ=255) ataCGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:5287180/1‑71 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc > 1:666016/1‑70 (MQ=255) taCGACGGGTCATCATTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCcttc > 1:5462633/1‑70 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGcc < 1:2983383/71‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc < 1:3307086/70‑1 (MQ=255) cgacgGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGc < 1:4796101/70‑1 (MQ=255) gggTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCg < 1:3866528/71‑1 (MQ=255) gTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa < 1:1442612/71‑1 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGttctt < 1:1445814/50‑1 (MQ=255) catcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAt > 1:618336/1‑70 (MQ=255) atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:555288/1‑59 (MQ=255) atcatTCGGCCAGCAACCGAGACTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCctt > 1:4256860/1‑59 (MQ=255) atcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAAtt < 1:686803/70‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTc < 1:3931232/64‑1 (MQ=255) tcatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:4701572/71‑1 (MQ=255) taatGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCttt > 1:5677460/3‑58 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa < 1:4416385/65‑1 (MQ=255) catGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg > 1:4585570/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg > 1:5287455/1‑48 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:923928/70‑1 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:4031458/70‑1 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGc > 1:2425460/1‑71 (MQ=255) atGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCCTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTg < 1:4652666/70‑1 (MQ=255) atGAGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCg > 1:4521076/1‑48 (MQ=255) gCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGt > 1:17456/1‑71 (MQ=255) ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTg < 1:1099486/70‑1 (MQ=255) ggCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGc < 1:3994730/71‑1 (MQ=255) ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTg < 1:5267452/51‑1 (MQ=255) ccAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:48892/1‑71 (MQ=255) gCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg > 1:714320/1‑71 (MQ=255) cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:2577827/1‑55 (MQ=255) cAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGaa > 1:3238388/1‑55 (MQ=255) aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt > 1:3061736/1‑71 (MQ=255) aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:807830/71‑1 (MQ=255) aaCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt > 1:3421109/1‑71 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:3417545/70‑1 (MQ=255) aCGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTATGCGTGCAATTGGt > 1:1375951/1‑70 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc < 1:2081012/39‑1 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTtctc < 1:5345666/39‑1 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:700856/1‑63 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:1073703/1‑63 (MQ=255) ggCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:3461357/1‑63 (MQ=255) gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:4214467/67‑1 (MQ=255) gCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCa > 1:851014/1‑69 (MQ=255) gACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAgg > 1:4700983/1‑71 (MQ=255) aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag < 1:5408656/67‑1 (MQ=255) aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag < 1:2462445/67‑1 (MQ=255) aCTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag < 1:2590850/67‑1 (MQ=255) cTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTa > 1:2552620/1‑71 (MQ=255) cTTCAATGTTTAAGGATTCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTa < 1:3182353/71‑1 (MQ=255) ttCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGa < 1:5508224/44‑1 (MQ=255) ttCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:1077842/49‑1 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTAt < 1:3968678/69‑1 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:3159777/1‑71 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:874971/1‑71 (MQ=255) cGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa > 1:2747049/1‑71 (MQ=255) gATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGa < 1:2304240/70‑1 (MQ=255) aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:4991798/58‑1 (MQ=255) aTGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATc < 1:813340/71‑1 (MQ=255) ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGt < 1:1739618/55‑1 (MQ=255) ttCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc < 1:3862576/70‑1 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAGTTCTGCGTGCAATTGGt > 1:759464/1‑53 (MQ=255) cAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc > 1:5045988/1‑71 (MQ=255) aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCt < 1:3900552/39‑1 (MQ=255) aGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGAc < 1:4588395/70‑1 (MQ=255) cgcTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACgg > 1:527619/1‑70 (MQ=255) ttCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg < 1:4128052/45‑1 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt < 1:1378233/71‑1 (MQ=255) ttCCAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAAt < 1:1887922/71‑1 (MQ=255) tCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg > 1:1254626/1‑44 (MQ=255) tCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTg > 1:797670/1‑44 (MQ=255) ccAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg > 1:5144277/1‑71 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCaa > 1:420245/1‑39 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATcgc < 1:3077494/59‑1 (MQ=255) cAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCg < 1:4192689/70‑1 (MQ=255) cAGTTCCTGGTTATCCTTCGCATCAAACTCTTCGTGCAACTGGTCAGAGGTATGGTCGCGGCGAAAATcat < 1:2879435/71‑3 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTc > 1:2170955/1‑44 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTc > 1:4882857/1‑44 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt < 1:782698/71‑1 (MQ=255) aGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGtt < 1:4859584/71‑1 (MQ=255) tcttcGTTCTCTTTTCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTc > 1:4883809/1‑69 (MQ=255) tcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTgg > 1:3570621/1‑39 (MQ=255) tcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTgg > 1:2926051/1‑39 (MQ=255) tcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTgg > 1:2670841/1‑39 (MQ=255) tcttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTgg > 1:4479582/1‑39 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCaga > 1:2786365/1‑43 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCag > 1:3327098/1‑42 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGaaa > 1:5671097/1‑62 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATAATCGCGACGGAAATc > 1:1217364/1‑64 (MQ=255) cttcGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGAATGATCGCGACGGAAATCGTTCgg > 1:3036027/1‑70 (MQ=255) | TACCCATAATACGACGGGTCATCATGCGGCCAGCAACGGCGACTTCGATGTTCAGCGCTTCCAGTTCTTCGTTCTCTTTGCCGTCGAATTCTGCGTGCAATTGGTCAGAGGTATGATCGCGACGGAAATCGTTCGG > minE/1934199‑1934334 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |