Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 1,934,443 G→T 5.2% intergenic (‑1/+9) lysS ← / ← prfB lysine tRNA synthetase, constitutive/peptide chain release factor RF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE1,934,4430GT 5.2% 438.3 / 5.2 133intergenic (‑1/+9)lysS/prfBlysine tRNA synthetase, constitutive/peptide chain release factor RF‑2
Reads supporting (aligned to +/- strand):  ref base G (62/64);  new base T (4/3);  total (66/67)
Fisher's exact test for biased strand distribution p-value = 7.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.29e-01

Only 100 of 134 total aligned reads displayed.

ACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTCCAGCACGGCC  >  minE/1934374‑1934512
                                                                     |                                                                     
acgacgCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGt                                                                      <  1:3925754/71‑1 (MQ=255)
  gacgCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTg                                                                    >  1:5014997/1‑71 (MQ=255)
    cgcgTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGt                                                                  >  1:2386891/1‑71 (MQ=255)
    cgcgTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGt                                                                  >  1:5535691/1‑71 (MQ=255)
     gcgTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGt                                                                  <  1:2663085/70‑1 (MQ=255)
        ttttCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGtt                                                                     <  1:153581/64‑1 (MQ=255)
          ttCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCt                                                              >  1:3034023/1‑69 (MQ=255)
          ttCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTc                                                             <  1:5632178/70‑1 (MQ=255)
          ttCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTc                                                             <  1:2189667/70‑1 (MQ=255)
          ctCAGTTCATCGTTAAAATCAATAGCCTCATTGGCTCCCCGTGTTTCTTGTTCAGACATTTTGGTTCCTc                                                             >  1:578697/2‑70 (MQ=255)
           tCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTgg                                                                   >  1:3891516/1‑63 (MQ=255)
           tCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTgg                                                                   >  1:4556817/1‑63 (MQ=255)
           tCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCa                                                            >  1:5435641/1‑70 (MQ=255)
            cAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCAt                                                           >  1:3677705/1‑70 (MQ=255)
            cAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCAt                                                           <  1:5570318/70‑1 (MQ=255)
            cAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTATTCAGACATGTTGGTTCCTCAt                                                           <  1:786975/70‑1 (MQ=255)
             aGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCt                                                              <  1:755313/66‑1 (MQ=255)
              gTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGATCCTCAt                                                           <  1:5330529/68‑1 (MQ=255)
                    tGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGc                                                   <  1:2237655/70‑1 (MQ=255)
                    tGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCt                                                  >  1:2498608/1‑71 (MQ=255)
                     gTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGtt                                                                     >  1:2771501/1‑51 (MQ=255)
                     gTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCtt                                                 >  1:2818421/1‑71 (MQ=255)
                      ttAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCttt                                                >  1:72308/1‑71 (MQ=255)
                        aaGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTgg                                                                   >  1:1076268/1‑50 (MQ=255)
                         aGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCaa                                             <  1:834854/71‑1 (MQ=255)
                         aGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCa                                              >  1:4296872/1‑70 (MQ=255)
                         aGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCa                                              >  1:1564852/1‑70 (MQ=255)
                         aGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCa                                              >  1:4225649/1‑70 (MQ=255)
                          gATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCaaa                                            >  1:4535199/1‑71 (MQ=255)
                           aTCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGtt                                                                     <  1:3848722/45‑1 (MQ=255)
                           aTCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGtt                                                                     <  1:1727476/45‑1 (MQ=255)
                             cGACTACCGCGTCAGCGGCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAAcc                                                       >  1:4115554/1‑57 (MQ=255)
                             cGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCAt                                                           >  1:2518334/1‑53 (MQ=255)
                              gACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTg                                        >  1:1033511/1‑71 (MQ=255)
                               aCTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCt                                                     <  1:1701514/57‑1 (MQ=255)
                                   ccGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTTGTTCCTCATAACCCTGCTTTCAAACTTGCTTCg                                   <  1:702221/71‑1 (MQ=255)
                                   ccGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGc                                                   <  1:974870/55‑1 (MQ=255)
                                   ccGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTc                                    <  1:825099/70‑1 (MQ=255)
                                   ccGCGTCAGCGCCCTGTGCGTGGTGTTCAGACATGTTGGTTCCTCATAACCCTGCttt                                                >  1:5216609/1‑58 (MQ=255)
                                    cgcgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCttt                                                <  1:2775284/57‑1 (MQ=255)
                                     gcgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTg                                                    >  1:677378/1‑52 (MQ=255)
                                     gcgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTg                                                    >  1:4488863/1‑52 (MQ=255)
                                     gcgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCt                                                  >  1:1950824/1‑54 (MQ=255)
                                     gcgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGAt                                 <  1:4431912/71‑1 (MQ=255)
                                      cgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCa                                              >  1:554359/1‑57 (MQ=255)
                                      cgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATa                                <  1:4927776/71‑1 (MQ=255)
                                      cgTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATa                                <  1:106642/71‑1 (MQ=255)
                                       gTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATaa                               <  1:3369337/71‑1 (MQ=255)
                                       gTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATaa                               <  1:4171236/71‑1 (MQ=255)
                                          aGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACt                                          >  1:1004430/1‑57 (MQ=255)
                                          aGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAAtt                            >  1:2842665/1‑71 (MQ=255)
                                          aGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAAtt                            >  1:2655600/1‑71 (MQ=255)
                                          aGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAAtt                            <  1:2409280/71‑1 (MQ=255)
                                           gcgcCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTg                           <  1:163852/71‑1 (MQ=255)
                                           gcgcCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTg                           <  1:1117744/71‑1 (MQ=255)
                                           gcgcCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTg                           <  1:4147979/71‑1 (MQ=255)
                                            cgcCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGa                          <  1:3098763/71‑1 (MQ=255)
                                              cccTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGAt                         <  1:1149951/70‑1 (MQ=255)
                                              cccTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATc                        <  1:2118993/71‑1 (MQ=255)
                                               cccgtgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATcc                       <  1:4574346/68‑1 (MQ=255)
                                               ccTGTTCGTGTTGTTCAGACATGTTGGTTCCTCAt                                                           <  1:1614274/35‑1 (MQ=255)
                                               ccTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACtt                                         <  1:851853/53‑1 (MQ=255)
                                               ccTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACtt                                         <  1:3834789/53‑1 (MQ=255)
                                               ccTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATcc                       <  1:1018456/71‑1 (MQ=255)
                                                 tgtgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCaa                                             <  1:2929670/47‑1 (MQ=255)
                                                 tgtgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCaa                                             <  1:2135561/47‑1 (MQ=255)
                                                 tgtgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAg                     >  1:1974488/1‑71 (MQ=255)
                                                  gtgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCt                                                     >  1:3961753/1‑38 (MQ=255)
                                                  gtgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGa                                  >  1:1221037/1‑57 (MQ=255)
                                                  gtgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAgg                    >  1:5176471/1‑71 (MQ=255)
                                                   tgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATa                                <  1:922176/58‑1 (MQ=255)
                                                   tgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATa                                <  1:2461704/58‑1 (MQ=255)
                                                   tgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAg                     <  1:1441922/69‑1 (MQ=255)
                                                   tgCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATACAg                     <  1:3680357/69‑1 (MQ=255)
                                                    gCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCt                  >  1:5050459/1‑71 (MQ=255)
                                                     cGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGc                                       <  1:4702316/49‑1 (MQ=255)
                                                        gttgttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATaaa                              >  1:4708568/1‑55 (MQ=255)
                                                        gttgttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATaaa                              >  1:124858/1‑55 (MQ=255)
                                                        gttgttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATaaa                              >  1:131486/1‑55 (MQ=255)
                                                        gttgttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCg              >  1:4847623/1‑71 (MQ=255)
                                                        cttgttCAGACATTTTGGTTCCTCTAAATCCAGCTTTCaa                                             >  1:4846566/2‑40 (MQ=16)
                                                         ttgttCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATaaa                              <  1:5448536/54‑1 (MQ=255)
                                                         ttgttCAGACATTTTGGTTCCTCTAAATCCAGCTTTCAATTTAGCGTAGATaaa                              <  1:3793161/54‑1 (MQ=255)
                                                         ttgttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGt             <  1:3340178/71‑1 (MQ=255)
                                                         ttgttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGt             >  1:421256/1‑71 (MQ=255)
                                                         ttgttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGt             <  1:751729/71‑1 (MQ=255)
                                                           gttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTc            <  1:3236058/70‑1 (MQ=255)
                                                           gttCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTc            >  1:3484349/1‑70 (MQ=255)
                                                              cAGACATGTTGGTTCCTCATAACCCTGCTTTCAAAc                                           >  1:2737003/1‑36 (MQ=255)
                                                              cAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGc                                       >  1:4063969/1‑40 (MQ=255)
                                                              cAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATaaa                              >  1:288487/1‑49 (MQ=255)
                                                              cAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTCCAg         <  1:5652901/70‑1 (MQ=255)
                                                               aGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTCCAGc        <  1:4843447/70‑1 (MQ=255)
                                                               aGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTCCAGc        <  1:1401044/70‑1 (MQ=255)
                                                               aGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTCCAGCa       >  1:5215490/1‑71 (MQ=255)
                                                                 aCATGTTGGTTCCTCATAACCCTGCTTTCAAACTTTCTTCGATAAATTGa                          >  1:3209861/1‑50 (MQ=255)
                                                                   aTGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATaaa                              >  1:2647216/1‑44 (MQ=255)
                                                                   aTGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAAt                             <  1:175661/45‑1 (MQ=255)
                                                                   aTGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAAt                             <  1:4747173/45‑1 (MQ=255)
                                                                    tGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTCCAGCACGGcc  >  1:348606/1‑71 (MQ=255)
                                                                     |                                                                     
ACGACGCGTTTTCAGTTCATTGTTAAGATCGACTACCGCGTCAGCGCCCTGTGCGTGTTGTTCAGACATGTTGGTTCCTCATAACCCTGCTTTCAAACTTGCTTCGATAAATTGATCCAGGCTGCCGTCCAGCACGGCC  >  minE/1934374‑1934512

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: