| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,943,187 | A→C | 24.8% | K86N (AAA→AAC) ‡ | bglA → | 6‑phospho‑beta‑glucosidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,943,187 | 0 | A | C | 24.8% | 125.2 / 68.1 | 97 | K86N (AAA→AAC) ‡ | bglA | 6‑phospho‑beta‑glucosidase A |
| Reads supporting (aligned to +/- strand): ref base A (34/39); new base C (9/15); total (43/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.85e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
CCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAG > minE/1943118‑1943257 | ccATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt > 1:2176368/1‑71 (MQ=255)ccATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAAt > 1:5057066/1‑71 (MQ=255) aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGttt < 1:1281601/70‑1 (MQ=255) aaGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtt > 1:4994179/1‑69 (MQ=255) gCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCg > 1:5194858/1‑71 (MQ=255) gCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCg > 1:5188412/1‑71 (MQ=255) gTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTAc < 1:1646768/71‑1 (MQ=255) ttGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt < 1:2610467/65‑1 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtt > 1:2394812/1‑61 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTa > 1:1758445/1‑67 (MQ=255) gATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc > 1:4981092/1‑71 (MQ=255) ttttATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCAt > 1:2646213/1‑71 (MQ=255) gTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTg < 1:543105/70‑1 (MQ=255) tataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt < 1:495589/55‑1 (MQ=255) ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCa > 1:3619433/1‑57 (MQ=255) ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcgc > 1:2506636/1‑69 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTc > 1:4256618/1‑45 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:3264055/1‑71 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:253814/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt > 1:764619/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt < 1:1477950/71‑1 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGACGCGAAttt < 1:2554187/71‑1 (MQ=255) ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:2639763/51‑1 (MQ=255) ggAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:4451693/51‑1 (MQ=255) gAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt > 1:532169/1‑71 (MQ=255) gAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGtttt > 1:3709977/1‑42 (MQ=255) aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACAt < 1:1327729/48‑1 (MQ=255) aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt > 1:2585227/1‑71 (MQ=255) aCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACgcgc < 1:3753628/62‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1660478/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:3915062/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1202158/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:2737300/71‑1 (MQ=255) tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa < 1:2251707/71‑1 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:3208253/42‑1 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:5078748/1‑69 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:1561382/1‑69 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1166436/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1521333/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2398283/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:5079192/1‑71 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3382894/1‑71 (MQ=255) aaGCTATTTGCCAAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2704324/1‑52 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGc < 1:2787904/43‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:1014966/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2860158/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:1751890/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2951430/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCt < 1:2433945/45‑1 (MQ=255) ttATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTg > 1:2727003/2‑47 (MQ=255) tATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATc < 1:2929240/37‑1 (MQ=255) aTTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:2223538/1‑69 (MQ=255) aTTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt > 1:2868506/1‑70 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGacg > 1:2557695/1‑68 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5418485/1‑68 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5424863/1‑68 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5500840/55‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5487623/55‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5689610/59‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:2761226/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:3887882/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:1408563/71‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:2378942/71‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:373058/70‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:1182698/70‑1 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:2888843/1‑66 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg > 1:5497366/1‑71 (MQ=255) gCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtt > 1:1556532/1‑51 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:3346741/68‑1 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:5681630/68‑1 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAgtc < 1:1774428/71‑3 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt < 1:4237026/65‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt < 1:4395735/65‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc < 1:3439558/70‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:132510/71‑1 (MQ=255) gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:3975968/1‑69 (MQ=255) gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:1439941/1‑69 (MQ=255) aaTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGc > 1:3092252/1‑71 (MQ=255) tGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGcgacga < 1:2414136/61‑1 (MQ=255) gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa > 1:4804842/1‑70 (MQ=255) gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa > 1:40758/1‑70 (MQ=255) ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:3531599/70‑1 (MQ=255) ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:3538224/70‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:4422594/57‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:5446878/57‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:5023357/69‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCACATTTTTCCAAAAGGCGATg < 1:1586141/57‑1 (MQ=255) cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:293134/41‑1 (MQ=255) cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:4405322/41‑1 (MQ=255) cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:2040899/41‑1 (MQ=255) tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5015099/43‑1 (MQ=255) tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5401820/43‑1 (MQ=255) cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3078846/1‑48 (MQ=255) cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACg > 1:4576113/1‑68 (MQ=255) aaaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaag < 1:3468139/70‑1 (MQ=255) aaTGTTCTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAg > 1:12434/1‑45 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc > 1:4532978/1‑56 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc > 1:757194/1‑56 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag > 1:54746/1‑71 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag > 1:2320890/1‑71 (MQ=255) | CCATGAAGCCGTTGATTTTTATGGTCACTATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAG > minE/1943118‑1943257 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |