| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,943,212 | A→G | 12.6% | T95A (ACG→GCG) | bglA → | 6‑phospho‑beta‑glucosidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,943,212 | 0 | A | G | 12.6% | 292.2 / 28.6 | 119 | T95A (ACG→GCG) | bglA | 6‑phospho‑beta‑glucosidase A |
| Reads supporting (aligned to +/- strand): ref base A (55/49); new base G (7/8); total (62/57) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.84e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.46e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
ATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTT > minE/1943147‑1943282 | ataAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGAcgc > 1:2506636/1‑69 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:253814/1‑71 (MQ=255) aGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAtt > 1:3264055/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAttt > 1:764619/1‑71 (MQ=255) ggAAGATATCGCGTTATTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGACGCGAAttt < 1:2554187/71‑1 (MQ=255) aaGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttttt > 1:2585227/1‑71 (MQ=255) aCATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACgcgc < 1:3753628/62‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1202158/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:3915062/71‑1 (MQ=255) cATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaa < 1:1660478/71‑1 (MQ=255) tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa < 1:2251707/71‑1 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:5078748/1‑69 (MQ=255) cAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaa > 1:1561382/1‑69 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1521333/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:1166436/1‑52 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3382894/1‑71 (MQ=255) aaGCTATTTGCCAAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGa > 1:2704324/1‑52 (MQ=255) aTTTGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:2223538/1‑69 (MQ=255) aTTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt > 1:2868506/1‑70 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtttt < 1:4656174/56‑1 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5424863/1‑68 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5487623/55‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:5500840/55‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5689610/59‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:3887882/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:782863/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:2761226/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:2378942/71‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:1408563/71‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:373058/70‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:1182698/70‑1 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:2888843/1‑66 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg > 1:5497366/1‑71 (MQ=255) gCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAAtt > 1:1556532/1‑51 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:5681630/68‑1 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAgtc < 1:1774428/71‑3 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt < 1:4237026/65‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc < 1:3439558/70‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:132510/71‑1 (MQ=255) gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:1439941/1‑69 (MQ=255) gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:3975968/1‑69 (MQ=255) aaTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGc > 1:3092252/1‑71 (MQ=255) tGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGcgacga < 1:2414136/61‑1 (MQ=255) gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa > 1:4804842/1‑70 (MQ=255) gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa > 1:40758/1‑70 (MQ=255) ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:3531599/70‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:4422594/57‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:5446878/57‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:5023357/69‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCACATTTTTCCAAAAGGCGATg < 1:1586141/57‑1 (MQ=255) cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:293134/41‑1 (MQ=255) cTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCAttt < 1:4405322/41‑1 (MQ=255) tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5401820/43‑1 (MQ=255) tCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTcc < 1:5015099/43‑1 (MQ=255) cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3078846/1‑48 (MQ=255) aaaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaag < 1:3468139/70‑1 (MQ=255) aaTGTTTTCGTACATCCATTGCCTGGAGGCGCATTTTTCCAAAAGGCGATGAAGCTCAGcc > 1:3087242/1‑61 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc > 1:4532978/1‑56 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag > 1:54746/1‑71 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag > 1:2320890/1‑71 (MQ=255) gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:3968923/1‑46 (MQ=255) gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg > 1:828286/1‑71 (MQ=255) gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAgg > 1:5163031/1‑70 (MQ=255) ttttCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc < 1:4460066/53‑1 (MQ=255) tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:5515062/49‑1 (MQ=255) tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg > 1:2610421/1‑70 (MQ=255) tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg > 1:452740/1‑70 (MQ=255) tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg < 1:4410441/70‑1 (MQ=255) tACATCCATTGCCTGGACGCGCATTTTTACAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGt < 1:5561714/71‑1 (MQ=255) aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg < 1:5414125/37‑1 (MQ=255) aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa > 1:3119779/1‑68 (MQ=255) aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt > 1:35992/1‑71 (MQ=255) cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGttg < 1:3918682/71‑2 (MQ=255) cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc > 1:4727373/1‑71 (MQ=255) cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc > 1:5297251/1‑71 (MQ=255) cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc < 1:410016/71‑1 (MQ=255) tCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga > 1:4877372/1‑56 (MQ=255) tCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga > 1:5644705/1‑56 (MQ=255) ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc < 1:5413390/40‑1 (MQ=255) ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg > 1:1128385/1‑59 (MQ=255) ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc > 1:1567920/1‑71 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:1939601/1‑40 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc > 1:87697/1‑70 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc > 1:5328887/1‑70 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:1387380/70‑1 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:4206074/70‑1 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACg < 1:520970/71‑1 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAACGGCTGAAGTTCTa > 1:4928409/1‑69 (MQ=255) ttGCCTGGACGCGCATTTTTCCAAAAGGCTATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:2714317/68‑1 (MQ=255) tGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa > 1:3207257/1‑60 (MQ=255) tGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat > 1:3278914/1‑70 (MQ=255) ccTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatga < 1:3718369/70‑1 (MQ=255) tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:4498266/63‑1 (MQ=255) tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:2602028/63‑1 (MQ=255) ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa > 1:527416/1‑61 (MQ=255) ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa > 1:1129873/1‑61 (MQ=255) gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaaa > 1:44252/1‑39 (MQ=255) gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGatat > 1:5517412/1‑69 (MQ=255) gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGt > 1:3306118/1‑71 (MQ=255) aCGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGtt < 1:5131945/71‑1 (MQ=255) | ATAAGGAAGACATCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTT > minE/1943147‑1943282 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |