| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 1,943,229 | A→G | 7.7% | K100K (AAA→AAG) ‡ | bglA → | 6‑phospho‑beta‑glucosidase A |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 1,943,229 | 0 | A | G | 7.7% | 360.9 / 14.9 | 129 | K100K (AAA→AAG) ‡ | bglA | 6‑phospho‑beta‑glucosidase A |
| Reads supporting (aligned to +/- strand): ref base A (66/53); new base G (4/6); total (70/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.94e-01 | |||||||||||
Only 100 of 139 total aligned reads displayed.
TCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAA > minE/1943159‑1943298 | tCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCaaaa < 1:2251707/71‑1 (MQ=255) aaGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAgg > 1:3382894/1‑71 (MQ=255) aTTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt > 1:2868506/1‑70 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGacg > 1:2557695/1‑68 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5424863/1‑68 (MQ=255) tttGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGac > 1:5418485/1‑68 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:3887882/70‑1 (MQ=255) tttGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGa < 1:2378942/71‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:1182698/70‑1 (MQ=255) tGCCGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:373058/70‑1 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:2888843/1‑66 (MQ=255) tGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAg > 1:5497366/1‑71 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGaa < 1:3346741/68‑1 (MQ=255) ccGAGATGGGCTTCACCTGTCTGCGTATTTCCATTGCCTGGGCGCGAATTTTCCCTCAGGGCGACGAAgtc < 1:1774428/71‑3 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGAt < 1:4237026/65‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGc < 1:3439558/70‑1 (MQ=255) ccGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:132510/71‑1 (MQ=255) gAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:1439941/1‑69 (MQ=255) aaTGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGc > 1:3092252/1‑71 (MQ=255) gggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCa > 1:40758/1‑70 (MQ=255) ggCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:3538224/70‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATg < 1:4422594/57‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaa < 1:5023357/69‑1 (MQ=255) gCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCACATTTTTCCAAAAGGCGATg < 1:1586141/57‑1 (MQ=255) cAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACg > 1:4576113/1‑68 (MQ=255) aaaTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaag < 1:3468139/70‑1 (MQ=255) aaTGTTTTCGTACATCCATTGCCTGGAGGCGCATTTTTCCAAAAGGCGATGAAGCTCAGcc > 1:3087242/1‑61 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc > 1:757194/1‑56 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag > 1:2320890/1‑71 (MQ=255) aTGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGaagaag > 1:54746/1‑71 (MQ=255) gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGa > 1:3968923/1‑46 (MQ=255) gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg > 1:828286/1‑71 (MQ=255) gTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAgg > 1:5163031/1‑70 (MQ=255) ttttCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTc < 1:4460066/53‑1 (MQ=255) tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt < 1:5515062/49‑1 (MQ=255) tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg < 1:4410441/70‑1 (MQ=255) tCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTg > 1:452740/1‑70 (MQ=255) gTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAg > 1:889944/1‑71 (MQ=255) tACATCCATTGCCTGGACGCGCATTTTTACAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGt < 1:5561714/71‑1 (MQ=255) aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg < 1:3188849/37‑1 (MQ=255) aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCg < 1:5414125/37‑1 (MQ=255) aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa > 1:3119779/1‑68 (MQ=255) aCATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt > 1:35992/1‑71 (MQ=255) aCATCCATTGCCTGGACGCGCATTTATCCAAAAGGCg < 1:3012323/37‑1 (MQ=255) cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGttg < 1:3918682/71‑2 (MQ=255) cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc > 1:5297251/1‑71 (MQ=255) cATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTc > 1:4727373/1‑71 (MQ=255) tCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga > 1:4877372/1‑56 (MQ=255) tCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACgaaga > 1:5644705/1‑56 (MQ=255) ccATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAggg > 1:1128385/1‑59 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCt > 1:1939601/1‑40 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:1387380/70‑1 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc > 1:5328887/1‑70 (MQ=255) cATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:4206074/70‑1 (MQ=255) ttGCCTGGACGCGCATTTTTCCAAAAGGCTATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:2714317/68‑1 (MQ=255) tGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgat > 1:3278914/1‑70 (MQ=255) gCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACgatga > 1:1582035/1‑71 (MQ=255) tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:4498266/63‑1 (MQ=255) tGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTAc < 1:2602028/63‑1 (MQ=255) ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa > 1:527416/1‑61 (MQ=255) ggACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATg > 1:489374/1‑71 (MQ=255) gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaaa > 1:406420/1‑39 (MQ=255) gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCaaa > 1:44252/1‑39 (MQ=255) gACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGatat > 1:5517412/1‑69 (MQ=255) aCGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGtt < 1:5131945/71‑1 (MQ=255) cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc > 1:5013462/1‑71 (MQ=255) cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc < 1:3287107/71‑1 (MQ=255) cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc < 1:1179960/71‑1 (MQ=255) cgcgCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTc > 1:1742485/1‑71 (MQ=255) cgcATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGa < 1:3036843/71‑1 (MQ=255) gcATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa > 1:5697141/1‑49 (MQ=255) gcATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGaa > 1:720485/1‑49 (MQ=255) cATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATg < 1:4988778/71‑1 (MQ=255) aTTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAg < 1:4597890/48‑1 (MQ=255) aTTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAg < 1:4797649/48‑1 (MQ=255) aTTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAg < 1:652724/48‑1 (MQ=255) aTTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATg > 1:1125801/1‑70 (MQ=255) aTTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATg > 1:250038/1‑70 (MQ=255) tttttCCAAAAGGCGATGAAGCTCAGCCAAACgaag < 1:1103294/36‑1 (MQ=255) tttttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCt < 1:3942348/43‑1 (MQ=255) tttttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCt < 1:1462416/43‑1 (MQ=255) tttttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGata < 1:361333/60‑1 (MQ=255) ttttCCAAAAGGCGATGAAGCTTAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGa > 1:282214/1‑66 (MQ=255) ttttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt > 1:4741764/1‑48 (MQ=255) ttttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt > 1:80023/1‑48 (MQ=255) ttttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGtt > 1:2644877/1‑48 (MQ=255) ttttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAAc > 1:1181475/1‑71 (MQ=255) tttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa > 1:3207495/1‑50 (MQ=255) ttCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGa < 1:1905496/67‑1 (MQ=255) ccAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTa > 1:948559/1‑47 (MQ=255) cAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgctg < 1:3199517/71‑1 (MQ=255) cAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAActgctg < 1:764870/71‑1 (MQ=255) aaaGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGaa < 1:1726144/71‑1 (MQ=255) aaaGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGaa < 1:806231/71‑1 (MQ=255) aaaGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTATACga > 1:3859978/1‑47 (MQ=255) aaGGCTATGAAGCTCAGCCAAACGAATAAGGGCTGAAGTTCTAc < 1:378493/44‑1 (MQ=38) aaGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGaa > 1:1524891/1‑62 (MQ=255) aaGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGaa > 1:5476393/1‑62 (MQ=255) aaGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGaaa < 1:3512501/71‑1 (MQ=255) aaGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGaaa > 1:4092482/1‑71 (MQ=255) | TCAAGCTATTTGCCGAAATGGGCTTCAAATGTTTTCGTACATCCATTGCCTGGACGCGCATTTTTCCAAAAGGCGATGAAGCTCAGCCAAACGAAGAAGGGCTGAAGTTCTACGATGATATGTTCGATGAACTGCTGAAA > minE/1943159‑1943298 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |