| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,002,444 | C→A | 7.6% | intergenic (+91/+32) | yghU → / ← hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,002,444 | 0 | C | A | 7.6% | 286.5 / 5.6 | 92 | intergenic (+91/+32) | yghU/hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base C (44/40); new base A (3/4); total (47/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.08e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01 | |||||||||||
AGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGT > minE/2002375‑2002512 | aGCCATCCAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGcc > 1:300041/1‑71 (MQ=255)aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGc > 1:5213317/1‑70 (MQ=255) cATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCcgcg > 1:3412365/1‑71 (MQ=255) taGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCt > 1:1747449/2‑71 (MQ=11) cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCt > 1:547717/1‑70 (MQ=255) aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTa < 1:2424158/71‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCtt < 1:2369869/70‑1 (MQ=1) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTa > 1:5575257/1‑71 (MQ=1) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:398235/71‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:1976584/71‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTAt < 1:366548/71‑1 (MQ=1) ccGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCa < 1:2557997/71‑1 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAgg > 1:103501/1‑71 (MQ=1) aTAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAgac < 1:2140569/71‑3 (MQ=1) aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGcc > 1:299414/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTa > 1:502717/1‑71 (MQ=255) aGGCGTTCACGCCGCATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCt < 1:143691/70‑1 (MQ=11) ggCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGGCCTAc < 1:4056090/71‑1 (MQ=1) gCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGa < 1:5425052/71‑1 (MQ=255) cGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:4972006/71‑1 (MQ=255) cGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:2040050/71‑1 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:2818161/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:445070/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:2566937/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:5073097/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:1811793/1‑71 (MQ=255) cACGCCGCATCCGGCAATCGTTCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc < 1:2863666/70‑1 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGa > 1:448828/1‑66 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:1488499/1‑70 (MQ=255) aCGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCt < 1:270106/70‑1 (MQ=255) aCGCCGCATCCGGCAATCGAGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTa < 1:3990030/71‑1 (MQ=255) ccgcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTaa > 1:3214259/1‑69 (MQ=255) ccgcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatc < 1:5619053/71‑1 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:1283253/71‑1 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:1862813/71‑1 (MQ=255) ccGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcg > 1:853808/1‑71 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTaa > 1:1066588/1‑62 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc > 1:3899269/1‑71 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc > 1:2264226/1‑71 (MQ=255) gCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACgcgc > 1:313665/1‑71 (MQ=255) cAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCt > 1:3221371/1‑71 (MQ=255) cAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCt > 1:4051214/1‑71 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:4997144/52‑1 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:4606671/1‑71 (MQ=255) aTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg < 1:1021144/71‑1 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatc > 1:5196056/1‑58 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg > 1:4487556/1‑70 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGt < 1:5235391/71‑1 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat > 1:4082724/1‑59 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgcg > 1:3916511/1‑65 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGt < 1:5005017/70‑1 (MQ=255) gTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatca < 1:307170/57‑1 (MQ=255) gTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa > 1:2209611/1‑71 (MQ=255) tGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAAt < 1:3547729/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg < 1:3538600/70‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg < 1:3390203/70‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg < 1:2037908/70‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:887715/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:3471460/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:2162253/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt > 1:4828888/1‑71 (MQ=255) aca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:5081828/70‑1 (MQ=255) ca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACgcgc < 1:1787944/60‑1 (MQ=255) ca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:4726473/1‑62 (MQ=255) ca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCg < 1:615478/71‑1 (MQ=255) a‑aTGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCg > 1:3729234/1‑70 (MQ=255) aTGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg < 1:1094859/61‑1 (MQ=255) aTGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg < 1:3056775/61‑1 (MQ=255) gCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:1928746/49‑1 (MQ=255) gCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa > 1:3842880/1‑62 (MQ=255) gCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAAt > 1:3180678/1‑71 (MQ=255) ccTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAAt < 1:4275079/70‑1 (MQ=255) ccTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATc > 1:499980/1‑71 (MQ=255) ccTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATc > 1:506836/1‑71 (MQ=255) aTGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAg > 1:5421369/1‑36 (MQ=255) aTGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTaa < 1:16249/40‑1 (MQ=255) aTGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCa < 1:5367826/69‑1 (MQ=255) aTGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCa < 1:522617/69‑1 (MQ=255) aTGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCa > 1:4843897/1‑69 (MQ=255) aTGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAtt > 1:179636/1‑71 (MQ=255) aTGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAt < 1:3104497/70‑1 (MQ=255) tGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:2751855/43‑1 (MQ=255) tGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCAttt > 1:1786443/1‑71 (MQ=255) gCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc > 1:5508818/1‑47 (MQ=255) gCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa < 1:5073057/55‑1 (MQ=255) gCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa < 1:369893/55‑1 (MQ=255) gCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTg > 1:1637329/1‑71 (MQ=255) gCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTg > 1:2480610/1‑71 (MQ=255) cGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAAtcatca > 1:1548345/1‑43 (MQ=255) cGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatc > 1:1670158/1‑36 (MQ=255) cGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatc > 1:4582596/1‑36 (MQ=255) cGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa > 1:4973019/1‑51 (MQ=255) cGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGgcgc < 1:4883610/71‑1 (MQ=255) cGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGgcg > 1:3603018/1‑70 (MQ=255) ccGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCa < 1:1994584/58‑1 (MQ=255) ccGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGt < 1:2823075/69‑1 (MQ=255) | AGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCGTAATCCATTTGGCGCAGT > minE/2002375‑2002512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |