| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,040,713 | C→T | 71.3% | D40D (GAC→GAT) | yqiH → | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,040,713 | 0 | C | T | 71.3% | 137.2 / 70.8 | 101 | D40D (GAC→GAT) | yqiH | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base C (16/13); new base T (40/32); total (56/45) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.80e-01 | |||||||||||
Only 100 of 104 total aligned reads displayed.
CTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGA > minE/2040644‑2040781 | cTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGAc > 1:2993875/1‑70 (MQ=255)cTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGAc < 1:419919/70‑1 (MQ=255) aTGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAgg > 1:437376/1‑71 (MQ=255) aTGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAgg > 1:63719/1‑71 (MQ=255) tGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAgg < 1:3896839/70‑1 (MQ=255) gcagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCg > 1:4229056/1‑69 (MQ=255) cagcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAg > 1:5531291/1‑71 (MQ=255) gcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCa > 1:3483939/1‑71 (MQ=255) gcaTTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCa < 1:5197208/71‑1 (MQ=255) tGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTa > 1:4586187/1‑70 (MQ=255) gCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaa > 1:3420838/1‑70 (MQ=255) gCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaa < 1:1436613/70‑1 (MQ=255) gCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaa > 1:2861132/1‑70 (MQ=255) gCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTaa < 1:651076/70‑1 (MQ=255) ccTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaa < 1:4335640/70‑1 (MQ=255) ccTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaa < 1:792642/70‑1 (MQ=255) ccTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaa < 1:1885035/70‑1 (MQ=255) tGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACt > 1:2968218/1‑71 (MQ=255) gggTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACt < 1:3641484/69‑1 (MQ=255) gCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTaa > 1:383823/1‑71 (MQ=255) gCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTaa > 1:1231425/1‑71 (MQ=255) ccATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACaa > 1:3891850/1‑46 (MQ=255) atcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa < 1:5373964/71‑1 (MQ=255) tcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATaa < 1:1826918/43‑1 (MQ=255) tcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAg < 1:1810569/51‑1 (MQ=255) tcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAg < 1:229479/51‑1 (MQ=255) tcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa > 1:2754533/1‑70 (MQ=255) tcatcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa > 1:3151521/1‑70 (MQ=255) catcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTa > 1:2181694/1‑62 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGc < 1:3579068/50‑1 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGc < 1:1143437/50‑1 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:2640228/1‑71 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:5170603/1‑71 (MQ=255) atcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:5528527/1‑71 (MQ=255) atcCCGGATCGTACCCGTATCAAAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAg > 1:2191793/1‑71 (MQ=255) tcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACt > 1:707717/1‑58 (MQ=255) tcCCGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGt < 1:4777192/71‑1 (MQ=255) tcCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTaa < 1:821514/64‑1 (MQ=255) cccGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaaa > 1:4104647/1‑55 (MQ=255) cccGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaaa > 1:181324/1‑55 (MQ=255) cccGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaaa > 1:4871220/1‑55 (MQ=255) cccGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACtt < 1:3662611/58‑1 (MQ=255) cccGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGt > 1:4182031/1‑70 (MQ=255) ccGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCa < 1:2915699/65‑1 (MQ=255) cGGATCGTACCCGTATCATAATGAATGAGTCAGAc < 1:625450/35‑1 (MQ=255) cGGATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaa < 1:3472980/71‑1 (MQ=255) ggATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa < 1:1577379/71‑1 (MQ=255) ggATCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa < 1:3891554/71‑1 (MQ=255) gATCGTACACGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa < 1:5601418/71‑1 (MQ=255) aTCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaa > 1:3977572/1‑67 (MQ=255) aTCGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAAc < 1:5191645/71‑1 (MQ=255) cGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACt > 1:4419975/1‑54 (MQ=255) cGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACt > 1:2816021/1‑54 (MQ=255) cGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa > 1:790069/1‑67 (MQ=255) cGTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa > 1:5594576/1‑67 (MQ=255) gTACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCt < 1:3030952/70‑1 (MQ=255) tACCCTTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaa < 1:3189162/64‑1 (MQ=255) tACCCGTATCATAATGAATGAGTCAGATAAAGGCGAAg < 1:1709363/38‑1 (MQ=255) cccGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGccc > 1:962432/1‑71 (MQ=255) ccGTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCt < 1:1895207/71‑1 (MQ=255) ccGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCt < 1:1606915/71‑1 (MQ=255) gTATCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTAt > 1:1517989/1‑71 (MQ=255) aTCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAg < 1:5607749/53‑1 (MQ=255) tCATAATGAATGAGTCAGATAAAGGCGAAGCATTaaa < 1:4428931/37‑1 (MQ=255) tCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa > 1:2514242/1‑57 (MQ=255) tCATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa > 1:4290152/1‑57 (MQ=255) tCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCt < 1:1328881/70‑1 (MQ=255) tCATAATGAATGAGTCAGACAAACGCGAAGCATTaa < 1:1354498/36‑1 (MQ=255) tCATAATGAATGAATCAGATAAAGGCGAAGCATTaaa < 1:1984899/37‑1 (MQ=38) cATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTgg > 1:5407799/1‑71 (MQ=255) cATAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTgg > 1:5358253/1‑71 (MQ=255) cATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCt < 1:4701448/69‑1 (MQ=255) aTAATGAATGAGTCAGATAAAGGCGAAGCATTAAAAc > 1:708791/1‑37 (MQ=255) aTAATGAATGAGTCAGATAAAGGCGAAGCATTAAAAc > 1:3880911/1‑37 (MQ=255) aTAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATc > 1:3648247/1‑67 (MQ=255) aTAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGc > 1:2885734/1‑71 (MQ=255) tAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc < 1:840087/71‑1 (MQ=255) tAATGAATGAGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGcc < 1:2855168/71‑1 (MQ=255) tAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTAc < 1:979333/40‑1 (MQ=255) tAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTAc < 1:4199807/40‑1 (MQ=255) aatgaatgAGTCAGATAAAGGCGAAGCATTAAAACtt > 1:5226847/1‑37 (MQ=255) aatgaatgAGTCAGATAAAGGCGAAGCATTAAAACTTa > 1:1849502/1‑38 (MQ=255) aatgaatgAGTCAGATAAAGGCGAAGCATTAAAACTTa > 1:1409175/1‑38 (MQ=255) aatgaatgAGTCAGATAAAGGCGAAGCATTAAAACTTa > 1:2350524/1‑38 (MQ=255) aatgaatgAGTCAGATAAAGGCGAAGCATTAAAACTTa > 1:1620490/1‑38 (MQ=255) aatgaatgAGTCAGATAAAGGCGAAGCATTAAAACTTa > 1:4351073/1‑38 (MQ=255) atgaatgaGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGccc < 1:1287071/70‑1 (MQ=255) atgaatgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCa < 1:231056/71‑1 (MQ=255) tgaatgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCa > 1:1966648/1‑70 (MQ=255) tgaatgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCa > 1:5076649/1‑70 (MQ=255) gaatgaGTCAGATAAAGGCGAAGCATTAAAACTTACTAATc > 1:2840409/1‑41 (MQ=255) gaatgaGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAAc > 1:1342980/1‑52 (MQ=255) gaatgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaa > 1:1082257/1‑50 (MQ=255) atgaGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTaaaaa > 1:2042800/1‑49 (MQ=255) atgaGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACa < 1:1356822/71‑1 (MQ=255) atgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACa < 1:3791898/71‑1 (MQ=255) tgaGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTAt > 1:4544348/1‑58 (MQ=255) gaGTCAGATAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATg > 1:1911728/1‑71 (MQ=255) cAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTTCCCTATCTGGCCCAAACATGGa > 1:5646023/1‑69 (MQ=255) gACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATaga < 1:5530946/71‑1 (MQ=255) | CTGTATGAGCAGCATTGCCTGGGGTGCCATCATCCCGGATCGTACCCGTATCATAATGAATGAGTCAGACAAAGGCGAAGCATTAAAACTTACTAATCAAAGTAAAAACCTGCCCTATCTGGCCCAAACATGGATAGA > minE/2040644‑2040781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |