| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,040,951 | T→A | 86.0% | *120K (TAA→AAA) | yqiH → | predicted periplasmic pilin chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,040,951 | 0 | T | A | 86.0% | 241.5 / 28.3 | 93 | *120K (TAA→AAA) | yqiH | predicted periplasmic pilin chaperone |
| Reads supporting (aligned to +/- strand): ref base T (8/5); new base A (43/37); total (51/42) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.66e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01 | |||||||||||
GAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAA > minE/2040882‑2041018 | gAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTa < 1:1545278/71‑1 (MQ=255) tGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGAt > 1:4140306/1‑70 (MQ=255) tCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTa < 1:30574/60‑1 (MQ=255) cAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaaaa > 1:252128/1‑61 (MQ=255) cAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCaaaa > 1:4507597/1‑61 (MQ=255) aaCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaa > 1:949379/1‑70 (MQ=255) aaCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaa < 1:1976451/70‑1 (MQ=255) aaCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaa > 1:4094490/1‑70 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaaa < 1:4845257/71‑1 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaaa < 1:4061243/71‑1 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaa > 1:1626675/1‑70 (MQ=255) aCTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaa > 1:469536/1‑70 (MQ=255) cTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaaaa > 1:14895/1‑71 (MQ=255) ccTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgt > 1:2271008/1‑71 (MQ=255) cTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgt < 1:5538508/70‑1 (MQ=255) cTAAAGACAGAGAAACGCTCTTCTATTATAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgt < 1:3074206/70‑1 (MQ=255) aaaGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCAAAAACAGATAAAAAAAATGTGAt > 1:4656484/1‑71 (MQ=255) aaGACAGTGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATg > 1:3044505/1‑71 (MQ=255) aaGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATg > 1:4427728/1‑71 (MQ=255) aaGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGAAAAAAAAAATGTGATg > 1:544134/1‑71 (MQ=255) aGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGc < 1:121405/71‑1 (MQ=255) aCAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGc > 1:2665961/1‑69 (MQ=255) gaAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCaa > 1:2928321/1‑66 (MQ=255) gaAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCAc < 1:3326745/71‑1 (MQ=255) aaaCGCTCATCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGa > 1:1980783/1‑60 (MQ=255) aaCGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa < 1:3300712/71‑1 (MQ=255) aCGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaaa < 1:2899372/52‑1 (MQ=255) cGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:973466/1‑69 (MQ=255) cttctATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcag > 1:4222836/1‑70 (MQ=255) ttctATTTTAACGTGCGAGAAATACCGCCCTAAACAg < 1:1398104/37‑1 (MQ=255) ttctATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcag < 1:2574218/69‑1 (MQ=255) ctATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACg > 1:2757046/1‑71 (MQ=255) ctATTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACg > 1:3052610/1‑71 (MQ=255) aTTTTAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCa > 1:3293080/1‑71 (MQ=255) ttttAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaaa < 1:465384/41‑1 (MQ=255) ttttAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaa < 1:1333994/40‑1 (MQ=255) ttttAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgt < 1:1777105/45‑1 (MQ=255) ttttAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAAtgt < 1:1793537/45‑1 (MQ=255) ttttAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc < 1:4933773/62‑1 (MQ=255) ttAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:1382952/1‑57 (MQ=255) ttAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgca > 1:1027463/1‑61 (MQ=255) ttAACGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCAtt < 1:5232331/70‑1 (MQ=255) tAACGTGCTAGAAATACCGCCCAAAACAGATaaaaaa < 1:4526558/37‑1 (MQ=38) tAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaaaa > 1:2047600/1‑39 (MQ=255) tAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaa < 1:4228065/37‑1 (MQ=255) tAACGTGCGAGAAATACCGCCCAAAACAGATaaaaaa < 1:131361/37‑1 (MQ=255) tAACGTGCGAGAAATACCGCCCAAAACAGATaaaaa < 1:2272427/36‑1 (MQ=255) aaCGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGa < 1:3705534/70‑1 (MQ=255) aaCGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGa < 1:1588540/70‑1 (MQ=255) aCGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGaa < 1:5141504/70‑1 (MQ=255) aCGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcag < 1:4779672/59‑1 (MQ=255) aCGTGCGAGAAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcag < 1:2196288/59‑1 (MQ=255) gagaAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCa < 1:2689914/45‑1 (MQ=255) gagaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTc > 1:2546609/1‑71 (MQ=255) gagaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTc > 1:2236794/1‑71 (MQ=255) agaAATACCGCCCAAAACAGATAAAAATAATGTGATGCAAGTCACTAt < 1:2528908/48‑1 (MQ=39) agaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTAt < 1:5203648/48‑1 (MQ=255) agaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCAtt < 1:2496125/60‑1 (MQ=255) gaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcag < 1:3733287/51‑1 (MQ=255) gaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcag < 1:592354/51‑1 (MQ=255) gaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcag < 1:4014255/51‑1 (MQ=255) gaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTg < 1:1656529/71‑1 (MQ=255) gaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTg > 1:1896953/1‑71 (MQ=255) gaAATACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTg < 1:3789965/71‑1 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc > 1:5458824/1‑48 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc > 1:2432233/1‑48 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc > 1:621530/1‑48 (MQ=255) aaaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATgc > 1:3803767/1‑48 (MQ=255) aaaTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTc > 1:2421758/1‑68 (MQ=255) aaaTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTgg < 1:1253119/71‑1 (MQ=255) aaTACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggc > 1:3184319/1‑71 (MQ=255) aaTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTgg < 1:1165512/70‑1 (MQ=255) aTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAg > 1:4775813/1‑37 (MQ=255) aTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCAGTATGCAGCACGCATTGa > 1:830547/1‑58 (MQ=255) aTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGa > 1:3690931/1‑58 (MQ=255) aTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGa > 1:2152186/1‑58 (MQ=255) aTACCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGa > 1:4818377/1‑58 (MQ=255) aCCGCCCAAAACAGATAAAAAAAATGTGATGCAAg > 1:4849516/1‑35 (MQ=255) aCCGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:5200793/1‑41 (MQ=255) aCCGCCAAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:3822707/1‑41 (MQ=38) ccGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:2185637/1‑40 (MQ=255) ccGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:586735/1‑40 (MQ=255) ccGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:210396/1‑40 (MQ=255) ccGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:222910/1‑40 (MQ=255) ccGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:3149543/1‑40 (MQ=255) ccGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTggcggc > 1:2478858/1‑70 (MQ=255) ccGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTgg > 1:1001429/1‑66 (MQ=255) cGCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATgcagca > 1:5495889/1‑46 (MQ=255) gCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCaa < 1:2762356/71‑1 (MQ=255) gCCCAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCaa < 1:1511934/71‑1 (MQ=255) cccAAAACAGATAAAAAAAATGTGATGCAAGTCAc > 1:4146054/1‑35 (MQ=255) cccAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:120105/1‑37 (MQ=255) cccAAAACAGATAAAAAAAATGTGATGCAAGTCACTa > 1:4309006/1‑37 (MQ=255) cccAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCaaa < 1:424342/71‑1 (MQ=255) cccAAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCaa > 1:3159723/1‑70 (MQ=255) | GAAAAAATTGCTCAACTTCCTAAAGACAGAGAAACGCTCTTCTATTTTAACGTGCGAGAAATACCGCCCTAAACAGATAAAAAAAATGTGATGCAAGTCACTATGCAGCACGCATTGAAACTATTCTGGCGGCCAAA > minE/2040882‑2041018 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |