| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,045,853 | T→C | 22.1% | intergenic (+615/+180) | yqiK → / ← rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,045,853 | 0 | T | C | 22.1% | 258.2 / 62.5 | 122 | intergenic (+615/+180) | yqiK/rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base T (46/49); new base C (12/15); total (58/64) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.28e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.71e-01 | |||||||||||
Only 100 of 124 total aligned reads displayed.
CAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCAT > minE/2045792‑2045921 | cagcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAg > 1:830053/1‑71 (MQ=255) agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTa < 1:2531398/62‑1 (MQ=255) agcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTa < 1:4329956/62‑1 (MQ=255) gcaAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTatg > 1:4328361/1‑63 (MQ=255) aGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTa > 1:3736880/1‑58 (MQ=255) aGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTc < 1:657273/69‑1 (MQ=255) gggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGTCCTTGTATAAGGAAAGGGTTa < 1:1977594/56‑1 (MQ=255) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat > 1:5627124/1‑71 (MQ=255) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat > 1:552372/1‑71 (MQ=255) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat > 1:3187602/1‑71 (MQ=255) cgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGtca < 1:1786500/70‑1 (MQ=1) cTGAAACGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatg < 1:144998/55‑1 (MQ=14) gAAACGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat > 1:1896669/1‑67 (MQ=14) gAAACGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat > 1:1889915/1‑67 (MQ=14) gAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGtt < 1:1190765/50‑1 (MQ=255) aaaCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatgat < 1:3787364/54‑1 (MQ=14) aaaCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatgat < 1:4403377/54‑1 (MQ=14) aaaCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg < 1:565443/71‑3 (MQ=11) aaaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATAc > 1:4899675/1‑71 (MQ=255) aaCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAg > 1:3535738/1‑57 (MQ=255) aCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCAtgatga > 1:3590871/1‑54 (MQ=14) aCGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAATGAAAGGGTTATGATGAAGCTCGt < 1:5193989/61‑1 (MQ=255) ggAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatgat < 1:2622584/49‑1 (MQ=14) ggAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtg > 1:1155474/1‑70 (MQ=2) gAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt > 1:3680797/1‑70 (MQ=11) gAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc > 1:3332284/1‑69 (MQ=2) cccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt < 1:1253870/60‑1 (MQ=255) tcTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtg < 1:3123410/63‑2 (MQ=11) ccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt > 1:3907027/1‑70 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc < 1:1663603/50‑1 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc < 1:3373786/50‑1 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa < 1:4919004/71‑1 (MQ=255) tCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCat < 1:128161/36‑1 (MQ=12) tCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCat < 1:2965762/36‑1 (MQ=12) cccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCtac > 1:74665/1‑44 (MQ=9) cccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGc > 1:3127908/1‑43 (MQ=255) ccGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga > 1:3161910/1‑59 (MQ=2) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatga > 1:5472944/1‑36 (MQ=12) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatga > 1:1660542/1‑36 (MQ=12) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:213885/52‑1 (MQ=9) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:2184869/52‑1 (MQ=9) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:3118384/52‑1 (MQ=9) ccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt < 1:2337461/66‑1 (MQ=255) cGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatgat < 1:3931266/36‑1 (MQ=9) cGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCatgat < 1:3598393/36‑1 (MQ=9) cGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:1020149/1‑52 (MQ=255) cGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg < 1:2294832/55‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:525181/1‑51 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:4698006/71‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGt > 1:5507266/1‑70 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt > 1:1201610/1‑55 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:3440667/1‑70 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa < 1:1849759/71‑1 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg > 1:4303137/1‑53 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg > 1:3598080/1‑53 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa < 1:4262499/71‑1 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa < 1:1283014/71‑1 (MQ=255) gaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg > 1:5332259/1‑71 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg < 1:5080620/57‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg < 1:3400364/57‑1 (MQ=255) gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg < 1:4397732/56‑1 (MQ=255) gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc < 1:2279448/71‑1 (MQ=255) gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc < 1:2599328/71‑1 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg < 1:5123806/44‑1 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:4961894/1‑71 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:5610479/1‑71 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:2174414/1‑71 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa > 1:3449558/1‑52 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt > 1:2513298/1‑54 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt < 1:2989076/70‑1 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCAACATACTg > 1:4741596/1‑40 (MQ=255) tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt < 1:2037146/62‑1 (MQ=255) tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt < 1:2360843/70‑1 (MQ=255) tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt < 1:4805201/70‑1 (MQ=255) ataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAc > 1:3781916/1‑71 (MQ=255) ataAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt < 1:177545/40‑1 (MQ=255) ataAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt < 1:4983593/40‑1 (MQ=255) taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAc > 1:3135905/1‑70 (MQ=255) taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt < 1:4184059/71‑1 (MQ=255) taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga < 1:2103245/41‑3 (MQ=14) taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga < 1:1334239/41‑3 (MQ=14) aaGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt > 1:1233939/1‑70 (MQ=255) aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa > 1:385031/1‑54 (MQ=255) aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa > 1:486568/1‑54 (MQ=255) aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa > 1:315198/1‑54 (MQ=255) gAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa < 1:3086171/61‑1 (MQ=255) aaaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa > 1:1408650/1‑71 (MQ=255) aaaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa < 1:1078939/71‑1 (MQ=255) aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTGCTGTTAAGTTTCCCGACTTACTaacaa > 1:777201/1‑70 (MQ=255) aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt > 1:1940289/1‑54 (MQ=255) aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGtt > 1:5557866/1‑53 (MQ=255) aGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACt < 1:1435832/71‑1 (MQ=255) aGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACt < 1:4297176/71‑1 (MQ=255) gggTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:4734498/1‑57 (MQ=255) ggTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa > 1:2129616/1‑67 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:1968655/71‑1 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:1948681/71‑1 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:2787557/71‑1 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:2724373/71‑1 (MQ=255) tATGATGACGAGCTTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:4135275/69‑1 (MQ=25) | CAGCAAGGGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCAT > minE/2045792‑2045921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |