| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,045,866 | G→C | 8.8% | intergenic (+628/+167) | yqiK → / ← rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,045,866 | 0 | G | C | 8.8% | 364.7 / 18.8 | 125 | intergenic (+628/+167) | yqiK/rfaE | conserved hypothetical protein/fused heptose 7‑phosphate kinase and heptose 1‑phosphate adenyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (56/57); new base C (6/5); total (62/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
GGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAA > minE/2045799‑2045935 | acgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGt > 1:303292/3‑70 (MQ=0) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat > 1:552372/1‑71 (MQ=255) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat > 1:5627124/1‑71 (MQ=255) ggCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtcat > 1:3187602/1‑71 (MQ=255) cgCTGAAACGGGAAAGCCTCTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGtca < 1:1786500/70‑1 (MQ=1) gAAACGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat > 1:1896669/1‑67 (MQ=14) gAAACGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACtcat > 1:1889915/1‑67 (MQ=14) aaaCGGGAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcatcg < 1:565443/71‑3 (MQ=11) ggAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgtt > 1:3793091/1‑71 (MQ=255) gAAAGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc > 1:3332284/1‑69 (MQ=2) gAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttg < 1:2081641/71‑1 (MQ=255) aaaGCCCCTCCCGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTCATCATCGTGgtgc > 1:3132334/1‑68 (MQ=2) cccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACt < 1:1253870/60‑1 (MQ=255) tcTCCCGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtg < 1:3123410/63‑2 (MQ=11) ccTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttgt > 1:3907027/1‑70 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGtc < 1:3373786/50‑1 (MQ=255) cTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa < 1:4919004/71‑1 (MQ=255) cccGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:4898656/1‑54 (MQ=255) ccGGAGAAGAGGGCTTTTAATAAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga > 1:3161910/1‑59 (MQ=2) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:2184869/52‑1 (MQ=9) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:3118384/52‑1 (MQ=9) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:213885/52‑1 (MQ=9) ccGAGGAAG‑GGGCCATAAATAAGGAAAGGGTCATGATGAAGCTACTcatcat < 1:3479904/52‑1 (MQ=9) cGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:1020149/1‑52 (MQ=255) cGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg < 1:2294832/55‑1 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATa > 1:525181/1‑51 (MQ=255) gaagaaG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:4307788/71‑1 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgt > 1:1201610/1‑55 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt > 1:3440667/1‑70 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa < 1:1849759/71‑1 (MQ=255) aagaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa < 1:2702621/71‑1 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg > 1:611677/1‑53 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTgg > 1:3598080/1‑53 (MQ=255) agaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa < 1:4262499/71‑1 (MQ=255) gaag‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg > 1:5332259/1‑71 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg < 1:3400364/57‑1 (MQ=255) g‑gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg < 1:5080620/57‑1 (MQ=255) gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGgttgtgttg < 1:4397732/56‑1 (MQ=255) gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa < 1:5556436/66‑1 (MQ=255) gggCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa < 1:2402884/66‑1 (MQ=255) gCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTccc < 1:2279448/71‑1 (MQ=255) ccTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTg < 1:5123806/44‑1 (MQ=255) cTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:4961894/1‑71 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTa > 1:3449558/1‑52 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACt > 1:2513298/1‑54 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt < 1:2989076/70‑1 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt > 1:5045282/1‑70 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACt > 1:1802981/1‑70 (MQ=255) gTATAAGGAAAGGGTTATGATGAAGCTCGTCAACATACTg > 1:4741596/1‑40 (MQ=255) tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt < 1:2360843/70‑1 (MQ=255) tataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACtt < 1:4805201/70‑1 (MQ=255) aataAGGAAAGGGTTATGATGAAGCACGTCATCATACTgg > 1:1658768/2‑40 (MQ=14) ataAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAc > 1:3781916/1‑71 (MQ=255) ataAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt < 1:1232592/40‑1 (MQ=255) ataAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt < 1:4983593/40‑1 (MQ=255) ataAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgt < 1:177545/40‑1 (MQ=255) taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTAc > 1:3135905/1‑70 (MQ=255) taAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt < 1:4184059/71‑1 (MQ=255) taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga < 1:5329727/41‑3 (MQ=14) taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga < 1:1334239/41‑3 (MQ=14) taAGGAAAGGGTTATGATGAAGCACGTCATCATACTGgtga < 1:2103245/41‑3 (MQ=14) aaGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACt > 1:1233939/1‑70 (MQ=255) aGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa > 1:486568/1‑54 (MQ=255) aaaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa < 1:1078939/71‑1 (MQ=255) aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTGCTGTTAAGTTTCCCGACTTACTaacaa > 1:777201/1‑70 (MQ=255) aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGttt > 1:1940289/1‑54 (MQ=255) aaGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGtt > 1:5557866/1‑53 (MQ=255) aGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACt < 1:1435832/71‑1 (MQ=255) aGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACt < 1:4297176/71‑1 (MQ=255) gggTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:4734498/1‑57 (MQ=255) ggTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa > 1:1308732/1‑67 (MQ=255) ggTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaacaa > 1:2129616/1‑67 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:2787557/71‑1 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:1948681/71‑1 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:1968655/71‑1 (MQ=255) gTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:2724373/71‑1 (MQ=255) tATGATGACGAGCTTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat < 1:4135275/69‑1 (MQ=25) atgatgAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACtcat > 1:4483074/1‑68 (MQ=255) tgatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:4025806/40‑1 (MQ=255) tgatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGtt < 1:3335732/40‑1 (MQ=255) gatgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTaac < 1:84775/59‑1 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTaa > 1:135141/1‑40 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa > 1:3961127/1‑39 (MQ=255) atgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTa > 1:1475997/1‑53 (MQ=255) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTa < 1:5675658/38‑1 (MQ=255) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg > 1:2793077/1‑40 (MQ=255) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg > 1:1020266/1‑40 (MQ=255) tgaAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg > 1:3742105/1‑40 (MQ=255) tgaAGCACGTCATCATACTGGTGATACTCTTAGTGATTAGCTTCCAGGCTTACTaagaac < 1:526827/60‑1 (MQ=255) aaGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg > 1:3223184/1‑38 (MQ=255) aaGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg < 1:3028699/71‑1 (MQ=255) aaGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg > 1:2282737/1‑71 (MQ=255) aGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGa > 1:4745058/1‑45 (MQ=255) aGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAggggg > 1:483794/1‑70 (MQ=255) gCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAg > 1:1470959/1‑36 (MQ=255) gCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGga < 1:4943335/71‑1 (MQ=255) gCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGga < 1:4607599/71‑1 (MQ=255) cTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGgag < 1:1903942/71‑1 (MQ=255) gTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGaa < 1:66579/70‑1 (MQ=255) gTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGaa < 1:1102412/70‑1 (MQ=255) | GGGCTGAAACGGGAAAGCCCCTCCCGAAGAAG‑GGGCCTTGTATAAGGAAAGGGTTATGATGAAGCTCGTCATCATACTGGTTGTGTTGTTACTGTTAAGTTTCCCGACTTACTAACAACTCATCAGAGGGGGGAGAA > minE/2045799‑2045935 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |