| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,053,925 | A→C | 8.3% | intergenic (+83/+98) | cca → / ← bacA | fused tRNA nucleotidyl transferase and 2'3'‑cyclic phosphodiesterase/2'nucleotidase/phosphatase/undecaprenyl pyrophosphate phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,053,925 | 0 | A | C | 8.3% | 156.3 / 7.5 | 60 | intergenic (+83/+98) | cca/bacA | fused tRNA nucleotidyl transferase and 2'3'‑cyclic phosphodiesterase/2'nucleotidase/phosphatase/undecaprenyl pyrophosphate phosphatase |
| Reads supporting (aligned to +/- strand): ref base A (30/25); new base C (3/2); total (33/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.73e-01 | |||||||||||
TGCCGGATGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGAC > minE/2053856‑2053993 | tGCCGGATGCGGCGTAACCGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCa < 1:4998899/70‑1 (MQ=255) gCCGGATGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAg > 1:415947/1‑71 (MQ=255) gCCGGATGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAg > 1:1407079/1‑71 (MQ=255) ggATGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGt < 1:4212813/71‑1 (MQ=255) ggATGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGt < 1:5082094/71‑1 (MQ=255) tGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGc < 1:1081898/71‑1 (MQ=255) tGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGc < 1:92199/71‑1 (MQ=255) aaCGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGt > 1:1842913/1‑71 (MQ=255) aCGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGGAAGCGTCGCATCAGGCGtt > 1:3875910/1‑71 (MQ=255) aCGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGtt < 1:129242/71‑1 (MQ=255) cGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGtt > 1:3281464/1‑70 (MQ=255) gCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGa > 1:328791/1‑71 (MQ=255) ttATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGtt < 1:1417792/66‑1 (MQ=255) ttATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTg > 1:5198770/1‑71 (MQ=255) tATCCGGCCTACAGGTCGGCAACAGTTGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCattgattg > 1:4402974/1‑71 (MQ=2) aTCCGGCCTACAGGTCGGCAACAGTTGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGc > 1:3497838/1‑71 (MQ=2) aTCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCa < 1:2195553/46‑1 (MQ=255) aTCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCa < 1:3992913/46‑1 (MQ=255) aTCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTg < 1:1559422/69‑1 (MQ=255) ggccTACAGGTCGGCAACAGTTGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGCCGGa > 1:5129818/4‑71 (MQ=14) gg‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGAt > 1:2698069/1‑71 (MQ=255) g‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGt < 1:5031998/46‑1 (MQ=255) g‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGt < 1:3971231/46‑1 (MQ=255) g‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGt < 1:720948/46‑1 (MQ=255) cATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCg > 1:968648/1‑44 (MQ=255) aTGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGAtt < 1:3024428/58‑1 (MQ=255) aTGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATgcgg < 1:1681334/69‑1 (MQ=255) tGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATgcggc < 1:1625650/69‑1 (MQ=255) gTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAg > 1:5472320/1‑36 (MQ=255) gTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGt > 1:4160627/1‑70 (MQ=255) cAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGaa > 1:4671056/1‑71 (MQ=255) aaCGACAGTTGTAGGCCTGATAAGACGCGCAAGCGt > 1:296514/1‑36 (MQ=255) aaCGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAAc > 1:2545792/1‑71 (MQ=255) cGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAg > 1:778063/1‑42 (MQ=255) cGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGc > 1:688297/1‑71 (MQ=255) cGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGc < 1:483099/71‑1 (MQ=255) gtttGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGCCGGATGCGGCGTAAACGCCtt < 1:3890482/68‑1 (MQ=11) gtttGTAGGCCTGATAAGACGCGACAGCGTCGCATCAGGCATTGATTGCCGGATGCGGCGTAAACGCCtt < 1:4293478/68‑1 (MQ=11) aGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTa < 1:1025895/71‑1 (MQ=255) tttGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGc < 1:3924670/38‑1 (MQ=37) gTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCg < 1:4145213/50‑1 (MQ=255) gTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTAt < 1:5202251/71‑1 (MQ=255) ttGTAGGCCTGATAAGACGCGCAAGCGTCGCATCa > 1:3462187/1‑35 (MQ=37) ttGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGCAt < 1:4536747/52‑1 (MQ=255) tGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAAc > 1:2172949/1‑62 (MQ=255) gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGCGGCGTAAACGCCTTATCCg > 1:11775/1‑71 (MQ=14) gTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGCGGCGTAAACGCCTTATCCg > 1:384209/1‑71 (MQ=14) ggCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGcc < 1:3706205/71‑1 (MQ=255) cTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTAc > 1:4226647/1‑71 (MQ=255) tGATAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGCGGCGta < 1:4217731/51‑2 (MQ=14) gATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACg > 1:5380770/1‑70 (MQ=255) aTAAGACGCGCAAGCGTCGCATCAGGCGTTGGTTGCCGAATGAGGCGTAAACGCCTTAt > 1:243636/1‑59 (MQ=17) aTAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGc > 1:4815312/1‑70 (MQ=255) aaGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAAt > 1:1385256/1‑71 (MQ=255) aGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATc < 1:5636696/71‑1 (MQ=255) gACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCg > 1:5637974/1‑71 (MQ=255) aCGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATgcggc < 1:1660086/41‑1 (MQ=255) aCGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGt > 1:3671525/1‑71 (MQ=255) cgcgCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGtt > 1:3079450/1‑71 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGa > 1:1416237/1‑70 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGa > 1:946860/1‑70 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGAc < 1:5568931/71‑1 (MQ=255) gcAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGAc < 1:4357513/71‑1 (MQ=255) | TGCCGGATGCGGCGTAAACGCCTTATCCGG‑CTACATGTCAACGACAGTTGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCGTTGATTGCCGGATGCGGCGTGAACGCCTTATTCGGCCTACGCAATCGTTGAC > minE/2053856‑2053993 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |