| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,523 | C→T | 28.7% | S450S (TCG→TCA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,523 | 0 | C | T | 28.7% | 179.8 / 71.1 | 105 | S450S (TCG→TCA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (36/38); new base T (14/16); total (51/54) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
Only 100 of 107 total aligned reads displayed.
GTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCT > minE/311455‑311589 | gTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa < 1:2636719/71‑1 (MQ=255) aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg > 1:4973468/1‑71 (MQ=255) aTAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAg > 1:5367023/1‑71 (MQ=255) tAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACa < 1:679377/69‑1 (MQ=255) agagAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGa < 1:788602/66‑1 (MQ=255) agagAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTa > 1:1350901/1‑71 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:4286376/71‑1 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:3284158/71‑1 (MQ=255) gaACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa < 1:1460261/71‑1 (MQ=255) aaCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:3447088/71‑1 (MQ=9) aCTGGCGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCAt < 1:2281518/70‑1 (MQ=9) aCTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATc < 1:567990/71‑1 (MQ=255) cTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcg > 1:3512703/1‑71 (MQ=255) gcGATAAATTGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa < 1:2261693/70‑1 (MQ=12) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:4104221/63‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:2462337/63‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:4097488/63‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:13856/63‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgcg < 1:372901/70‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgcg < 1:61448/70‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:4589323/69‑1 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:2999704/1‑71 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:3048474/1‑71 (MQ=255) aCGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGa > 1:3423818/1‑71 (MQ=255) gatagataGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGAt < 1:5466771/70‑1 (MQ=255) gataaattGCCCCAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATa < 1:4363444/71‑1 (MQ=255) gatagCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGa > 1:2544643/1‑71 (MQ=255) agCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGAc > 1:2910826/1‑69 (MQ=255) gccccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATa > 1:3179630/4‑63 (MQ=12) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:1459092/70‑1 (MQ=255) gCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa < 1:4451474/70‑1 (MQ=255) cccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAcc < 1:1448029/51‑1 (MQ=12) cccAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCAcc < 1:5367771/51‑1 (MQ=12) cAGTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTgc > 1:186789/1‑70 (MQ=12) cAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCagag > 1:3760587/1‑69 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa > 1:4201841/1‑50 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCa > 1:3184393/1‑50 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:5664523/1‑71 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAgcgc > 1:254290/1‑71 (MQ=255) aGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGAAAGTACCATCGCGATACCGACCAGAgcgc > 1:3427005/1‑71 (MQ=255) gTAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGcc > 1:5383800/1‑71 (MQ=12) gTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGcc > 1:786918/1‑71 (MQ=255) tAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATc < 1:1082062/50‑1 (MQ=9) tAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATc < 1:5592764/50‑1 (MQ=9) tAGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATc < 1:3242049/50‑1 (MQ=9) tAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATa > 1:1141017/1‑56 (MQ=255) tAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGccc > 1:2052512/1‑71 (MQ=255) aGAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCt > 1:1240559/1‑71 (MQ=255) aGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:780975/1‑71 (MQ=255) gAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa > 1:5134930/1‑52 (MQ=9) gAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa > 1:1058364/1‑52 (MQ=9) gAACCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGa > 1:817718/1‑52 (MQ=9) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTAc < 1:2898020/44‑1 (MQ=255) gAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCt > 1:1090433/1‑70 (MQ=255) aaCCGCCGAAGAATGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTg > 1:5073295/1‑70 (MQ=9) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:260009/70‑1 (MQ=255) aCCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:718203/70‑1 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCGGACAGTACCATCGCGATACCGACCaga > 1:3391834/1‑58 (MQ=255) gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:2642158/71‑1 (MQ=255) cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGc < 1:593814/69‑1 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATcgcg > 1:5360285/1‑43 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc > 1:1816424/1‑71 (MQ=255) aagaagGCCATCGGTACGAATACCGCCGACAGTACCa < 1:3429659/37‑1 (MQ=255) aagaagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc < 1:2467516/47‑1 (MQ=255) agaagGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:1871110/40‑1 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGAcc > 1:4923411/1‑48 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCa > 1:4654972/1‑49 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTg < 1:3090746/65‑1 (MQ=255) agGCCATCGGTACGAATACCGCCGACAGTACCATcgc < 1:4894642/37‑1 (MQ=255) agGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATAcc < 1:1399055/43‑1 (MQ=255) tgCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:2156899/70‑1 (MQ=255) tgCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:457399/70‑1 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:5272128/71‑1 (MQ=255) gCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGa < 1:905006/57‑1 (MQ=255) cATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCTTCGACt > 1:1866184/1‑71 (MQ=255) aTCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt > 1:1423079/1‑70 (MQ=255) tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:3063381/1‑71 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc > 1:4732004/1‑55 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc > 1:5411668/1‑55 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:4652197/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCAACTTACg > 1:5107319/1‑71 (MQ=255) tACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACgg < 1:3657130/70‑1 (MQ=255) aCGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc < 1:775211/67‑1 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg > 1:370842/1‑69 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGATTTTGcga > 1:3464653/1‑51 (MQ=19) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:5636199/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:1861955/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:3019711/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:3216126/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:4848865/1‑59 (MQ=255) aTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt < 1:3574320/60‑1 (MQ=255) aTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt < 1:4281949/60‑1 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:2928484/47‑1 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:4381134/47‑1 (MQ=255) aCCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGAttt < 1:3507797/59‑1 (MQ=255) aCCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGAttt < 1:1629952/59‑1 (MQ=255) aCCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc > 1:433074/1‑71 (MQ=255) aCCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc > 1:1685943/1‑71 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGc > 1:2158641/1‑47 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttct > 1:1320568/1‑71 (MQ=255) | GTAATAGAGAACTGACGATAGATAGCACCAGTAGAACCGCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCT > minE/311455‑311589 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |