| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,068,638 | T→A | 12.4% | K384N (AAA→AAT) ‡ | aer ← | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,068,638 | 0 | T | A | 12.4% | 170.7 / 14.9 | 69 | K384N (AAA→AAT) ‡ | aer | fused signal transducer for aerotaxis sensory component and methyl accepting chemotaxis component |
| Reads supporting (aligned to +/- strand): ref base T (30/29); new base A (5/3); total (36/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.11e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCA > minE/2068566‑2068707 | gCGAATATCGTTCGCAGCATTAGCGCTGCGGCTGGCTAAATGA‑‑CACTTCCCCTGCCACCACTGCAAAACCt > 1:5477889/1‑71 (MQ=255) aaTATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt < 1:5128433/71‑1 (MQ=255) atatCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt > 1:642705/1‑71 (MQ=255) atatCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt < 1:3440332/71‑1 (MQ=255) tatCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg < 1:3794506/71‑1 (MQ=255) tagcGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt < 1:1950387/67‑1 (MQ=255) tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt > 1:1496479/1‑68 (MQ=255) tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt > 1:762290/1‑68 (MQ=255) tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCttt > 1:2017912/1‑68 (MQ=255) tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGcc < 1:5241137/71‑1 (MQ=255) tCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGcc > 1:1731936/1‑71 (MQ=255) ttGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTg < 1:509632/71‑1 (MQ=255) ttGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTg < 1:2189034/71‑1 (MQ=255) ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt > 1:1260742/1‑71 (MQ=255) ggCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGtt > 1:2271021/1‑71 (MQ=255) aTTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg < 1:1934680/71‑1 (MQ=255) ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGcc < 1:2562609/68‑1 (MQ=255) ttAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCgg < 1:4988295/70‑1 (MQ=255) tAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCa > 1:4627060/1‑71 (MQ=255) aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg > 1:3394627/1‑67 (MQ=255) aGCGCTGCGGCTGGCTAAATGCCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAc > 1:1497492/1‑71 (MQ=255) aGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCg > 1:288336/1‑67 (MQ=255) cgcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgc < 1:2795825/71‑1 (MQ=255) gcTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCAcgcg < 1:3653829/71‑1 (MQ=255) cTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACgcgc < 1:31476/71‑1 (MQ=255) tacGGCTGGCAAGATTGCGCACTTCCCCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCgagc > 1:949004/3‑70 (MQ=255) cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGc > 1:4130272/1‑49 (MQ=255) cGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc > 1:2561294/1‑58 (MQ=255) ctggctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCGTc > 1:5514198/1‑71 (MQ=255) ctAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc < 1:5691084/49‑1 (MQ=255) tAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt > 1:45832/1‑38 (MQ=255) tAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt > 1:4270326/1‑38 (MQ=255) tAAATGACGCACTTCCCCTGCCACCACTGCAAAACCtt > 1:289426/1‑38 (MQ=255) aaaTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTc < 1:96151/47‑1 (MQ=255) aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGt > 1:591358/1‑43 (MQ=255) aTGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGc > 1:3498637/1‑47 (MQ=255) tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg < 1:4226627/36‑1 (MQ=255) tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTg < 1:1648839/36‑1 (MQ=255) tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgccg > 1:2579055/1‑70 (MQ=255) tGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAccgcc > 1:4293917/1‑69 (MQ=255) aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGc > 1:2773435/1‑35 (MQ=255) aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc < 1:5137532/55‑1 (MQ=255) aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc < 1:3152915/55‑1 (MQ=255) aCGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGcgcc < 1:3223092/55‑1 (MQ=255) cGCACTTCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 1:4839485/71‑1 (MQ=255) ttCACCTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAggg > 1:4406751/1‑71 (MQ=255) tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCa > 1:3808880/4‑67 (MQ=255) tcaccTGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGc > 1:1607488/4‑71 (MQ=255) tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa > 1:3851941/1‑55 (MQ=255) tCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCa > 1:5501552/1‑55 (MQ=255) ccccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGc > 1:3133454/1‑70 (MQ=255) ccTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAg < 1:3115023/71‑1 (MQ=255) tGCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCAtt < 1:1804907/60‑1 (MQ=255) tGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa > 1:4876798/1‑71 (MQ=255) gCCACTACCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa > 1:551201/1‑70 (MQ=255) gCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGaa > 1:3128799/1‑70 (MQ=255) ccactaCCGCAAAACCACGCCCCTGCTCTCCGGCGCGAGCCGCTTCCACTGCCGCATTCAGGGCCAGaa < 1:1596229/69‑1 (MQ=255) ccaccaCTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCAc > 1:1004843/1‑49 (MQ=255) caccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat < 1:3381062/71‑1 (MQ=255) caccacAGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAatat > 1:1366067/1‑71 (MQ=255) ccacTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTg < 1:3391711/71‑1 (MQ=255) ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg < 1:5665247/71‑1 (MQ=255) ccacCGCAAAACCGCGCCCTTGCTCACCCGCACGCGCAGCCTCAACCGCCGCGTTCAAAGCCAGAATATTg > 1:2145271/1‑71 (MQ=255) acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCa > 1:872275/1‑55 (MQ=255) acTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGt > 1:1418717/1‑71 (MQ=255) cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTc < 1:983823/40‑1 (MQ=255) cTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTc > 1:4854925/1‑71 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAg > 1:560931/1‑52 (MQ=255) cAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTg < 1:3944400/70‑1 (MQ=255) aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:5113625/1‑61 (MQ=255) aaaaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAata > 1:915605/1‑61 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTc < 1:4460276/47‑1 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc < 1:3913188/71‑1 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc < 1:310011/71‑1 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc > 1:2298716/1‑71 (MQ=255) aaCCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAAc < 1:210974/71‑1 (MQ=255) ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACg > 1:2514079/1‑70 (MQ=255) ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc < 1:61465/71‑1 (MQ=255) ccTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGc < 1:1503705/71‑1 (MQ=255) cTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCa < 1:3548251/71‑1 (MQ=255) | GCGAATATCGTTGGCAGCATTAGCGCTGCGGCTGGCTAAATGACGCACTTCCCCTGCCACCACTGCAAAACCTTTGCCCTGTTCGCCGGCACGCGCCGCTTCCACCGCCGCATTCAGGGCCAGAATATTGGTCTGAAACGCA > minE/2068566‑2068707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |