Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,643 T→C 8.4% A403A (GCA→GCG)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,6430TC 8.4% 264.5 / 10.4 95A403A (GCA→GCG) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (49/38);  new base C (5/3);  total (54/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTCGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGC  >  minE/2093573‑2093710
                                                                      |                                                                   
tctcGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACt                                                                     <  1:2729347/71‑1 (MQ=255)
  tcGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGc                                                                   >  1:4391367/1‑71 (MQ=255)
  tcGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGc                                                                   >  1:445518/1‑71 (MQ=255)
  tcGATAACTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGc                                                                   >  1:2306192/1‑71 (MQ=255)
  tcGATAACTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGc                                                                   >  1:2839258/1‑71 (MQ=255)
  tcGATAACTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGc                                                                   >  1:2832423/1‑71 (MQ=255)
     aTCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCttt                                                                <  1:1398546/71‑1 (MQ=255)
       cACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCa                                                              >  1:5092501/1‑71 (MQ=255)
        aCTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCa                                                              <  1:3998911/70‑1 (MQ=255)
        aCTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCAc                                                             >  1:1138929/1‑71 (MQ=255)
        aCTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCAc                                                             >  1:5156758/1‑71 (MQ=255)
        aCTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGCtttta                                                              >  1:5467703/1‑68 (MQ=255)
        aCTTTATCGAGGCAGACGCGCGGCTCGCTGGTACGGCGCAGCGCCATACGTGCGGCGTTCACCGCtttta                                                              >  1:2238903/1‑68 (MQ=255)
                gAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTaa                                                     <  1:5292513/71‑1 (MQ=255)
                gAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTa                                                      <  1:4742627/70‑1 (MQ=255)
                gAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTa                                                      <  1:1870467/70‑1 (MQ=255)
                  gTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCAtt                                                       <  1:5099267/67‑1 (MQ=255)
                  gTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATg                                                   <  1:2313355/71‑1 (MQ=255)
                  gTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATg                                                   <  1:1792541/71‑1 (MQ=255)
                   tGAAAAACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATgg                                                  >  1:5601664/1‑71 (MQ=255)
                    gAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCAtt                                                       <  1:2256451/65‑1 (MQ=255)
                     aaaCACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACg                                                            >  1:2336679/1‑59 (MQ=255)
                     aaaCACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCa                                                >  1:5084048/1‑71 (MQ=255)
                      aaCACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAt                                               >  1:4501693/1‑71 (MQ=255)
                       acacGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAt                                               >  1:2520186/1‑70 (MQ=255)
                         acGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTAc                                            >  1:4586301/1‑71 (MQ=255)
                         acGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTAc                                            >  1:1049574/1‑71 (MQ=255)
                             ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:1307244/71‑1 (MQ=255)
                             ggTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:4699382/71‑1 (MQ=255)
                              gTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:109478/70‑1 (MQ=255)
                              gTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        >  1:1051328/1‑70 (MQ=255)
                               tGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                      <  1:4429569/71‑1 (MQ=255)
                                 ccGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATg                                    >  1:2188227/1‑71 (MQ=255)
                                  cGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACCGCt                                                                  <  1:1971276/40‑1 (MQ=255)
                                    aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                 >  1:3772774/1‑71 (MQ=255)
                                    aGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCAc                                 >  1:3369357/1‑71 (MQ=255)
                                      gTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCAtt                                                       <  1:662829/47‑1 (MQ=255)
                                        gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                             >  1:2599924/1‑71 (MQ=255)
                                        gcggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGa                             >  1:3882536/1‑71 (MQ=255)
                                         cggcgCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCa                                                         >  1:3578765/1‑42 (MQ=255)
                                              cATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGt                        <  1:1185664/70‑1 (MQ=255)
                                               aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:1292180/53‑1 (MQ=255)
                                               aTCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                      >  1:5358735/1‑71 (MQ=255)
                                                tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTaa                                                     >  1:4537388/1‑39 (MQ=255)
                                                tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                      >  1:759590/1‑54 (MQ=255)
                                                tCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                      >  1:5364519/1‑54 (MQ=255)
                                                  gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTa                                                      >  1:3830431/1‑36 (MQ=255)
                                                  gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAAt                                     >  1:3372029/1‑53 (MQ=255)
                                                  gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                   <  1:1356241/71‑1 (MQ=255)
                                                  gCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTg                   >  1:374560/1‑71 (MQ=255)
                                                   ccATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAc                      >  1:1770903/1‑67 (MQ=255)
                                                      tCCTCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAtt                           <  1:5050246/59‑1 (MQ=255)
                                                      tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCAtt                                              <  1:1651732/40‑1 (MQ=255)
                                                      tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAt                            <  1:5245108/58‑1 (MQ=255)
                                                      tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTATCTGTCCg               <  1:4469926/71‑1 (MQ=255)
                                                      tCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGt                  <  1:4249346/68‑1 (MQ=255)
                                                       ccGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGAttt                          >  1:3383283/1‑59 (MQ=255)
                                                        cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                         <  1:836306/43‑1 (MQ=255)
                                                        cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                         <  1:2241053/43‑1 (MQ=255)
                                                        cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGtt                                         <  1:1549572/43‑1 (MQ=255)
                                                        cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg              >  1:1152682/1‑70 (MQ=255)
                                                        cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg              >  1:1237253/1‑70 (MQ=255)
                                                        cgcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCgg              >  1:602812/1‑70 (MQ=255)
                                                         gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg            <  1:2659374/69‑2 (MQ=255)
                                                         gtgcggcgTTCACCGCTTTTACTGCCGCAATGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGcg            <  1:140230/69‑2 (MQ=255)
                                                         gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:3806820/43‑1 (MQ=255)
                                                         gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:5290970/43‑1 (MQ=255)
                                                         gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:2725174/43‑1 (MQ=255)
                                                         gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc             >  1:4318269/1‑70 (MQ=255)
                                                         gcgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGc             >  1:367462/1‑70 (MQ=255)
                                                          cgcgGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCa                                       >  1:5461145/1‑43 (MQ=255)
                                                             ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:37973/39‑1 (MQ=255)
                                                             ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:4747398/39‑1 (MQ=255)
                                                             ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg        <  1:3983882/71‑1 (MQ=255)
                                                             ggcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCg        <  1:2229390/71‑1 (MQ=255)
                                                              gcgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCgg       <  1:47870/71‑1 (MQ=255)
                                                               cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                      >  1:5543898/1‑39 (MQ=255)
                                                               cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCaa                                      >  1:5091175/1‑39 (MQ=255)
                                                               cgTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGa      >  1:4124898/1‑71 (MQ=255)
                                                                gTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt     >  1:2398074/1‑71 (MQ=255)
                                                                gTTTACTGCTTACACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGAt     >  1:4688571/1‑71 (MQ=255)
                                                                 tttACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:718600/35‑1 (MQ=255)
                                                                 tttACTGCTTTCACGGCATTAATGGCATTACGTTc                                        <  1:4501103/35‑1 (MQ=255)
                                                                 tttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCa            <  1:1033904/63‑1 (MQ=255)
                                                                  ttACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAAc          <  1:2483797/64‑1 (MQ=255)
                                                                   tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg   >  1:1807330/1‑70 (MQ=255)
                                                                   tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg   >  1:3354777/1‑70 (MQ=255)
                                                                   tACTGCTTTCACGGCATTAATGGAATTACGTTCAATGCACCGGATTTGTACCTg                   >  1:360010/1‑54 (MQ=38)
                                                                    aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACggg                              >  1:2728630/1‑42 (MQ=255)
                                                                    aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                     >  1:4997200/1‑51 (MQ=255)
                                                                    aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTAcc                     >  1:384749/1‑51 (MQ=255)
                                                                    aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg   >  1:428430/1‑69 (MQ=255)
                                                                    aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg   >  1:3965734/1‑69 (MQ=255)
                                                                    aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc  <  1:1246911/70‑1 (MQ=255)
                                                                    aCTGCTTTCACGGCATTAAAGGCATTACGTTCAATGCACGGGATTTGTAcc                     >  1:224919/1‑51 (MQ=255)
                                                                    aCTGCTTTCACGGAATTAATGGCATTACGTTCAATGCACggg                              >  1:1392820/1‑42 (MQ=255)
                                                                      tGCTTTCACGGCATTAATGGCATTACGTTCAATGCACgg                               <  1:327343/39‑1 (MQ=255)
                                                                      |                                                                   
TCTCGATCACTTTATCGAGTGAAACACGCGGTGCCGAGGTGCGGCGCATCGCCATCCGCGCGGCGTTTACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGC  >  minE/2093573‑2093710

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑