| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,093,709 | G→A | 16.6% | C381C (TGC→TGT) | tdcG ← | L‑serine dehydratase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,093,709 | 0 | G | A | 16.6% | 239.6 / 31.5 | 102 | C381C (TGC→TGT) | tdcG | L‑serine dehydratase 3 |
| Reads supporting (aligned to +/- strand): ref base G (43/42); new base A (13/4); total (56/46) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.35e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 112 total aligned reads displayed.
TACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCA > minE/2093640‑2093779 | tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg > 1:3354777/1‑70 (MQ=255)tACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg > 1:1807330/1‑70 (MQ=255) aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCg > 1:3965734/1‑69 (MQ=255) aCTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGc < 1:1246911/70‑1 (MQ=255) cTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt < 1:870489/70‑1 (MQ=255) ttCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAg > 1:3275880/1‑71 (MQ=255) tCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGc > 1:5304007/1‑71 (MQ=255) ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa < 1:1316666/71‑1 (MQ=255) ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa < 1:4238672/71‑1 (MQ=255) ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa < 1:4417386/71‑1 (MQ=255) cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg > 1:1290078/1‑71 (MQ=255) aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt > 1:62536/1‑71 (MQ=255) aTGGCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGaccg < 1:4700496/63‑2 (MQ=255) tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa < 1:2850388/62‑1 (MQ=255) tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa < 1:4082327/62‑1 (MQ=255) ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt > 1:5369122/1‑71 (MQ=255) ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt > 1:72115/1‑71 (MQ=255) ggCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCt > 1:2510150/1‑71 (MQ=255) gCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAg > 1:1661883/1‑51 (MQ=255) ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg < 1:5595479/60‑1 (MQ=255) ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg < 1:1899907/60‑1 (MQ=255) ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg < 1:3464394/60‑1 (MQ=255) ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt < 1:4430465/64‑1 (MQ=255) ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg < 1:5253238/60‑1 (MQ=255) ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt < 1:2779624/71‑1 (MQ=255) aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt > 1:2178079/1‑48 (MQ=255) aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc > 1:121262/1‑49 (MQ=255) aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTc > 1:511074/1‑49 (MQ=255) aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGccc > 1:4994782/1‑54 (MQ=255) aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg > 1:114394/1‑71 (MQ=255) aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAgac > 1:5432081/1‑51 (MQ=255) aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGtt > 1:605005/1‑60 (MQ=255) aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt > 1:346/1‑65 (MQ=255) aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg > 1:1677202/1‑69 (MQ=255) cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc < 1:1106021/71‑1 (MQ=255) cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc < 1:3410395/71‑1 (MQ=255) gTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGt < 1:2960225/46‑1 (MQ=255) cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa > 1:3693864/1‑56 (MQ=255) cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa > 1:4516193/1‑56 (MQ=255) cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa > 1:4494426/1‑56 (MQ=255) cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt > 1:3590660/1‑71 (MQ=255) cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt > 1:3584056/1‑71 (MQ=255) cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt > 1:1451747/1‑71 (MQ=255) cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt < 1:4537438/70‑1 (MQ=255) aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt < 1:1529390/71‑1 (MQ=255) tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt < 1:226556/51‑1 (MQ=255) tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt < 1:4015058/51‑1 (MQ=255) tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt < 1:4681619/51‑1 (MQ=255) gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt < 1:236864/53‑1 (MQ=255) cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc > 1:1744041/1‑71 (MQ=255) aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGc > 1:216264/1‑53 (MQ=255) cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg < 1:5343954/71‑1 (MQ=255) cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg < 1:381/71‑1 (MQ=255) gggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc > 1:1219149/1‑66 (MQ=255) aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa < 1:686516/45‑1 (MQ=255) aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa > 1:2327529/1‑45 (MQ=255) tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg > 1:1978175/1‑71 (MQ=255) tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt < 1:3523781/45‑1 (MQ=255) tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt < 1:3246839/45‑1 (MQ=255) tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc < 1:20459/50‑1 (MQ=255) tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc < 1:4156908/50‑1 (MQ=255) tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc < 1:5240538/50‑1 (MQ=255) ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg > 1:5319644/1‑70 (MQ=255) tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCa > 1:3967197/1‑49 (MQ=255) tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt > 1:2946154/1‑50 (MQ=255) gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTtgtg > 1:5605557/1‑43 (MQ=255) gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa > 1:2198475/1‑70 (MQ=12) gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa > 1:2405313/1‑70 (MQ=12) gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa < 1:1369682/70‑1 (MQ=255) ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc > 1:885311/1‑71 (MQ=255) cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt < 1:774486/67‑1 (MQ=255) cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt > 1:1351605/1‑62 (MQ=255) cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt > 1:3664762/1‑62 (MQ=255) cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt < 1:2939965/67‑1 (MQ=255) gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc > 1:810687/1‑67 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc > 1:2755031/1‑70 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt > 1:206288/1‑50 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt > 1:4332631/1‑50 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt > 1:2369391/1‑50 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg < 1:5115985/61‑1 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg < 1:1704363/61‑1 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg < 1:1450476/61‑1 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg < 1:3523794/61‑1 (MQ=255) tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt < 1:1091913/64‑1 (MQ=255) tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt < 1:5143837/64‑1 (MQ=255) ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc > 1:5102481/1‑53 (MQ=255) ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc > 1:5033261/1‑53 (MQ=255) ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc > 1:2417862/1‑53 (MQ=255) ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc > 1:3493960/1‑53 (MQ=255) cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt > 1:1296817/1‑48 (MQ=255) cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa > 1:4030355/1‑61 (MQ=255) cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc > 1:1777827/1‑71 (MQ=255) gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg < 1:5587325/68‑1 (MQ=9) gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg > 1:3939444/4‑71 (MQ=9) ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTg > 1:3450218/1‑59 (MQ=255) gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg < 1:1952331/60‑1 (MQ=255) gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg < 1:4934977/60‑1 (MQ=255) gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg < 1:2574925/60‑1 (MQ=255) gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg < 1:3908258/60‑1 (MQ=255) gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa < 1:3822503/71‑1 (MQ=255) | TACTGCTTTCACGGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCA > minE/2093640‑2093779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |