Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,721 A→G 15.6% L377L (CTT→CTC tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,7210AG15.6% 226.5 / 28.8 96L377L (CTT→CTCtdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (39/42);  new base G (12/3);  total (51/45)
Fisher's exact test for biased strand distribution p-value = 2.67e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.86e-01

Only 100 of 103 total aligned reads displayed.

GGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTA  >  minE/2093652‑2093791
                                                                     |                                                                      
ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                       <  1:1316666/71‑1 (MQ=255)
ggCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCaa                                                                       <  1:4417386/71‑1 (MQ=255)
  cATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                     >  1:1290078/1‑71 (MQ=255)
   aTTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:3518293/71‑1 (MQ=255)
      aaTGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 >  1:62536/1‑71 (MQ=255)
        tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                                        <  1:2850388/62‑1 (MQ=255)
        tGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCa                                                                        <  1:4082327/62‑1 (MQ=255)
         ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                              >  1:72115/1‑71 (MQ=255)
         ggCGTTACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                              >  1:5369122/1‑71 (MQ=255)
             ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                     <  1:1350301/60‑1 (MQ=255)
             ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                     <  1:1899907/60‑1 (MQ=255)
             ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                     <  1:3464394/60‑1 (MQ=255)
             ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAgg                                                                     <  1:5595479/60‑1 (MQ=255)
             ttACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 <  1:4430465/64‑1 (MQ=255)
             ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAgg                                                                     <  1:5253238/60‑1 (MQ=255)
             ttACGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAt                                                          <  1:2779624/71‑1 (MQ=255)
               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                        >  1:5653179/1‑71 (MQ=255)
               aCGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcg                                                        >  1:114394/1‑71 (MQ=255)
               aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGtt                                                                   >  1:605005/1‑60 (MQ=255)
               aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCt                                                              >  1:346/1‑65 (MQ=255)
               aCGCTCGATGCATGGCACCTGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCAtgg                                                        >  1:1677202/1‑69 (MQ=255)
                cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                       <  1:3410395/71‑1 (MQ=255)
                cGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATcgc                                                       <  1:1106021/71‑1 (MQ=255)
                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                  >  1:4494426/1‑56 (MQ=255)
                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                  >  1:4516193/1‑56 (MQ=255)
                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                  >  1:3693864/1‑56 (MQ=255)
                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                   >  1:3590660/1‑71 (MQ=255)
                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                   >  1:3584056/1‑71 (MQ=255)
                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAtt                                                   >  1:1451747/1‑71 (MQ=255)
                    cAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAt                                                    <  1:4537438/70‑1 (MQ=255)
                     aaTGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                  <  1:1529390/71‑1 (MQ=255)
                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:226556/51‑1 (MQ=255)
                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:4015058/51‑1 (MQ=255)
                       tGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGt                                                                    <  1:4681619/51‑1 (MQ=255)
                        gCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 <  1:236864/53‑1 (MQ=255)
                         cACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:1744041/1‑71 (MQ=255)
                          aCGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGc                                                               >  1:216264/1‑53 (MQ=255)
                           cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                            <  1:5343954/71‑1 (MQ=255)
                           cGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgccg                                            <  1:381/71‑1 (MQ=255)
                            gggATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                >  1:1219149/1‑66 (MQ=255)
                              gATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 <  1:4799612/47‑1 (MQ=255)
                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                  >  1:2327529/1‑45 (MQ=255)
                               aTTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTa                                                                  <  1:686516/45‑1 (MQ=255)
                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCGAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       >  1:1978175/1‑71 (MQ=255)
                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 <  1:3246839/45‑1 (MQ=255)
                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTAt                                                                 <  1:3523781/45‑1 (MQ=255)
                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                            <  1:5240538/50‑1 (MQ=255)
                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                            <  1:4156908/50‑1 (MQ=255)
                                tttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTcc                                                            <  1:20459/50‑1 (MQ=255)
                                 ttGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       >  1:5319644/1‑70 (MQ=255)
                                  tGTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCa                                                           >  1:3967197/1‑49 (MQ=255)
                                  tGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCAt                                                          >  1:2946154/1‑50 (MQ=255)
                                   gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTtgtg                                                                >  1:5605557/1‑43 (MQ=255)
                                   gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                     >  1:2405313/1‑70 (MQ=12)
                                   gTACCTGTCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCa                                     >  1:2198475/1‑70 (MQ=12)
                                   gTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                     <  1:1369682/70‑1 (MQ=255)
                                      ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                 >  1:885311/1‑71 (MQ=255)
                                       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt                                    <  1:774486/67‑1 (MQ=255)
                                       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                         >  1:3664762/1‑62 (MQ=255)
                                       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCAt                                         >  1:1351605/1‑62 (MQ=255)
                                       cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                    <  1:2939965/67‑1 (MQ=255)
                                         gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc                                  >  1:810687/1‑67 (MQ=255)
                                          tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTgcg                             >  1:2494244/1‑71 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc                              >  1:2755031/1‑70 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                  >  1:206288/1‑50 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                  >  1:4332631/1‑50 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                  >  1:2369391/1‑50 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       <  1:1450476/61‑1 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       <  1:1704363/61‑1 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       <  1:5115985/61‑1 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       <  1:3523794/61‑1 (MQ=255)
                                          tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                    <  1:1091913/64‑1 (MQ=255)
                                          tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                    <  1:5143837/64‑1 (MQ=255)
                                           ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:5102481/1‑53 (MQ=255)
                                           ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:2417862/1‑53 (MQ=255)
                                           ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:5033261/1‑53 (MQ=255)
                                           ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:3931271/1‑53 (MQ=255)
                                           ccGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTccgc                                              >  1:3493960/1‑53 (MQ=255)
                                            cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGAttt                                                  >  1:1296817/1‑48 (MQ=255)
                                            cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCa                                     >  1:4030355/1‑61 (MQ=255)
                                            cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                           >  1:5285012/1‑71 (MQ=255)
                                            cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                           >  1:1777827/1‑71 (MQ=255)
                                              gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                         >  1:3939444/4‑71 (MQ=9)
                                              gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                         <  1:5587325/68‑1 (MQ=9)
                                                    ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTg                               >  1:3450218/1‑59 (MQ=255)
                                                      aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgccgc                 <  1:3127635/71‑1 (MQ=255)
                                                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTcc                                                >  1:2162419/1‑37 (MQ=255)
                                                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                         <  1:4934977/60‑1 (MQ=255)
                                                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                         <  1:2574925/60‑1 (MQ=255)
                                                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                         <  1:1952331/60‑1 (MQ=255)
                                                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                         <  1:3908258/60‑1 (MQ=255)
                                                         gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa              <  1:3822503/71‑1 (MQ=255)
                                                          cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag             <  1:3440753/71‑1 (MQ=255)
                                                              tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt         <  1:3609381/71‑1 (MQ=255)
                                                              gcAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTg                                       <  1:5623993/40‑1 (MQ=255)
                                                                aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt        <  1:577449/70‑1 (MQ=255)
                                                                 gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGGATAGCATAc                                  >  1:322804/1‑43 (MQ=38)
                                                                    cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt    >  1:556013/1‑70 (MQ=255)
                                                                     aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa  <  1:1068627/71‑1 (MQ=255)
                                                                     aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa  <  1:1061863/71‑1 (MQ=255)
                                                                     |                                                                      
GGCATTAATGGCATTACGTTCAATGCACGGGATTTGTACCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTA  >  minE/2093652‑2093791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: