Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,093,757 T→C 11.5% V365V (GTA→GTG)  tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,093,7570TC11.5% 134.5 / 7.9 52V365V (GTA→GTG) tdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base T (26/20);  new base C (3/3);  total (29/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01

CCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCA  >  minE/2093690‑2093823
                                                                   |                                                                  
ccTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAcc                                                                 >  1:885311/1‑71 (MQ=255)
 cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGTGATTTCCGCCGCATTGCAt                                                                    <  1:774486/67‑1 (MQ=255)
 cTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                    <  1:2939965/67‑1 (MQ=255)
   gTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATAc                                                                  >  1:810687/1‑67 (MQ=255)
    tCCGGCGACCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTgcg                                                             >  1:2494244/1‑71 (MQ=255)
    tCCGGCAACCGGATCGCAGGTCAGGCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTgc                                                              >  1:2755031/1‑70 (MQ=255)
    tCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                    <  1:1091913/64‑1 (MQ=255)
    tCCGCCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCAt                                                                    <  1:5143837/64‑1 (MQ=255)
      cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                                           >  1:1777827/1‑71 (MQ=255)
      cGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGcc                                                           >  1:5285012/1‑71 (MQ=255)
        gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                                                         <  1:5587325/68‑1 (MQ=9)
        gcgaCCGGGTCACACGTCAGACCGAGGTTGTGCTCCATGGCGATTTCCGCCGCGATGCACACCTGCGCCgg                                                         >  1:3939444/4‑71 (MQ=9)
              ggATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTg                                                               >  1:3450218/1‑59 (MQ=255)
                aTCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTgccgc                                                 <  1:3127635/71‑1 (MQ=255)
                   gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                         <  1:1952331/60‑1 (MQ=255)
                   gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                         <  1:3908258/60‑1 (MQ=255)
                   gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                         <  1:2574925/60‑1 (MQ=255)
                   gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCgg                                                         <  1:4934977/60‑1 (MQ=255)
                   gCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCa                                              <  1:3822503/71‑1 (MQ=255)
                    cAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCag                                             <  1:3440753/71‑1 (MQ=255)
                        tCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagtagt                                         <  1:3609381/71‑1 (MQ=255)
                          aGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGtt                                        <  1:577449/70‑1 (MQ=255)
                           gCCCAAGGTTATGCTCCATCGCGATTTCCGCCGGATAGCATAc                                                                  >  1:322804/1‑43 (MQ=38)
                              cAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGt                                    >  1:556013/1‑70 (MQ=255)
                               aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                                  <  1:1068627/71‑1 (MQ=255)
                               aaGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTa                                  <  1:1061863/71‑1 (MQ=255)
                                aGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTaa                                 <  1:3242207/71‑1 (MQ=255)
                                 ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                     >  1:1757215/1‑66 (MQ=255)
                                 ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                     >  1:2795890/1‑66 (MQ=255)
                                 ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                     >  1:2639012/1‑66 (MQ=255)
                                 ggTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                     >  1:80479/1‑66 (MQ=255)
                                  gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                               <  1:4757352/71‑1 (MQ=255)
                                  gTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                               <  1:829348/71‑1 (MQ=255)
                                           cATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccg                      >  1:5469700/1‑71 (MQ=255)
                                            aTCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                     >  1:3856781/1‑71 (MQ=255)
                                             tCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                     >  1:2843138/1‑70 (MQ=255)
                                              cgcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCagta                                           >  1:4412515/1‑47 (MQ=255)
                                               gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGtt                                   >  1:682396/1‑54 (MQ=255)
                                               gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                               >  1:1375060/1‑58 (MQ=255)
                                               gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                               >  1:2489800/1‑58 (MQ=255)
                                               gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAAcc                               >  1:5362224/1‑58 (MQ=255)
                                               gcgATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAt                  >  1:849638/1‑71 (MQ=255)
                                                 gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCAtt                 >  1:4173286/1‑70 (MQ=255)
                                                 gATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTg                <  1:4048256/71‑1 (MQ=255)
                                                  aTTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTc                                       <  1:1469940/47‑1 (MQ=255)
                                                   tttCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTgccgccgc                     <  1:2904729/64‑1 (MQ=255)
                                                      ccgccgCGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGagc             <  1:4323993/69‑3 (MQ=255)
                                                      ccgccgCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACa            <  1:161852/70‑1 (MQ=255)
                                                       cgccgcGATGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCacg          >  1:4570746/1‑69 (MQ=255)
                                                            cATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCa                   >  1:2495466/1‑57 (MQ=255)
                                                              ttGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGc    >  1:4963297/1‑70 (MQ=255)
                                                               tGCACACCTGCGCCGGGCTTGCGCCTAATAGTTCTGCCAGACCAGCCGCCGCCATTGAGCAcgccacgcca  <  1:543722/71‑1 (MQ=255)
                                                                gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAg                                     >  1:2572045/1‑35 (MQ=255)
                                                                gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAAc             >  1:1229003/1‑59 (MQ=255)
                                                                gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa  <  1:4730031/70‑1 (MQ=255)
                                                                gCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCa  <  1:4668797/70‑1 (MQ=255)
                                                                   |                                                                  
CCTGTCCGGCAACCGGATCGCAGGTCAGCCCAAGGTTATGCTCCATCGCGATTTCCGCCGCATTGCATACCTGCGCCGGACTGCCGCCCAGTAGTTCAGTTAACCCTGCCGCCGCCATTGAACAGGCCACGCCA  >  minE/2093690‑2093823

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑