| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 311,562 | C→T | 20.7% | Q437Q (CAG→CAA) | acrB ← | multidrug efflux system protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 311,562 | 0 | C | T | 20.7% | 179.2 / 38.0 | 87 | Q437Q (CAG→CAA) | acrB | multidrug efflux system protein |
| Reads supporting (aligned to +/- strand): ref base C (36/33); new base T (7/11); total (43/44) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTT > minE/311493‑311630 | gCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:2642158/71‑1 (MQ=255) cgaagaatGCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGc < 1:593814/69‑1 (MQ=255) aaaGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcc > 1:1816424/1‑71 (MQ=255) aagGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTg < 1:3090746/65‑1 (MQ=255) tgCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:4761962/70‑1 (MQ=255) tgCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:457399/70‑1 (MQ=255) tgCCATCGGAATAAATACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCg < 1:2156899/70‑1 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:5518337/71‑1 (MQ=255) ggCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCg < 1:5272128/71‑1 (MQ=255) cATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCTTCGACt > 1:1866184/1‑71 (MQ=255) aTCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt > 1:1423079/1‑70 (MQ=255) tCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:3063381/1‑71 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc > 1:5411668/1‑55 (MQ=255) cGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATc > 1:4732004/1‑55 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg > 1:4652197/1‑71 (MQ=255) ggTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCAACTTACg > 1:5107319/1‑71 (MQ=255) tACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGcccc < 1:3768275/58‑1 (MQ=255) tACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACgg < 1:3657130/70‑1 (MQ=255) aCGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTAc < 1:775211/67‑1 (MQ=255) cGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACggg > 1:370842/1‑69 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGATTTTGcga > 1:3464653/1‑51 (MQ=19) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:4848865/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:3019711/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:3216126/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:5636199/1‑59 (MQ=255) aaTACCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGa > 1:1861955/1‑59 (MQ=255) aTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt < 1:3574320/60‑1 (MQ=255) aTACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt < 1:4281949/60‑1 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:2928484/47‑1 (MQ=255) tACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:4381134/47‑1 (MQ=255) aCCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGAttt < 1:3507797/59‑1 (MQ=255) aCCGCTGACAGCACCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGAttt < 1:1629952/59‑1 (MQ=255) aCCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc > 1:1685943/1‑71 (MQ=255) aCCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc > 1:433074/1‑71 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGc > 1:2158641/1‑47 (MQ=255) ccgccgACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttct > 1:1320568/1‑71 (MQ=255) gACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCt < 1:4342965/40‑1 (MQ=255) cAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGcttc > 1:3349190/1‑63 (MQ=255) aGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcgg > 1:246316/1‑71 (MQ=255) gcaCCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGATTTTTCCGTCGCTTCTTTTggc < 1:4178348/66‑1 (MQ=255) aCCATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGATTTTTCCGTCGCTTCTTTTggcgggag > 1:2750095/1‑68 (MQ=255) ccATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGAc > 1:5577918/1‑44 (MQ=255) ccATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGATTTTTCCGTCGCTTCttt < 1:714095/61‑1 (MQ=255) cATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATc < 1:3814667/40‑1 (MQ=16) cATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGatt < 1:2529745/44‑3 (MQ=16) cATCGCGATACCCACCAGTGCGCCCTGAATTTGCGACATCGatt < 1:4122318/44‑3 (MQ=16) aTCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCaa < 1:3859758/68‑1 (MQ=255) aTCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAAcc < 1:1911963/71‑1 (MQ=255) tCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACt < 1:2434777/42‑1 (MQ=255) gcgATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTa > 1:1941244/1‑42 (MQ=255) gcgATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttc > 1:914193/1‑71 (MQ=255) cgATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg < 1:2847855/43‑1 (MQ=255) aCCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg < 1:799649/39‑1 (MQ=255) aCCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACg < 1:1161061/39‑1 (MQ=255) ccGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:5651878/1‑51 (MQ=255) ccGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCtttt > 1:4673531/1‑51 (MQ=255) gACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCa > 1:954246/1‑71 (MQ=255) aCCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCttt < 1:4650574/47‑1 (MQ=255) aCCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCg < 1:2506615/67‑1 (MQ=255) aCCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCAt < 1:3774947/71‑1 (MQ=255) aCCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCAt < 1:72699/71‑1 (MQ=255) ccAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCAt < 1:2099848/70‑1 (MQ=255) ccAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCAt > 1:2593359/1‑70 (MQ=255) gagCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTggcggc < 1:3583454/50‑1 (MQ=255) gagCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttct < 1:953740/59‑1 (MQ=255) gcgcCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACg > 1:2943218/1‑71 (MQ=255) cgcCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGc < 1:3676133/71‑1 (MQ=255) ccTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAaca < 1:111315/65‑1 (MQ=255) ccTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAaca < 1:1490332/65‑1 (MQ=255) cTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCAt < 1:4912134/60‑1 (MQ=255) tGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCaa < 1:508183/44‑1 (MQ=255) tGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATaa > 1:4982303/1‑61 (MQ=255) tGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAAc > 1:1377659/1‑71 (MQ=255) tGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAAc > 1:4760678/1‑71 (MQ=255) tGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAAc > 1:3517069/1‑71 (MQ=255) gAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACctt > 1:1940471/1‑48 (MQ=255) gAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAAc > 1:1619275/1‑70 (MQ=255) gAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACg > 1:3677104/1‑71 (MQ=255) gAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACg > 1:4923046/1‑71 (MQ=255) gAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACg > 1:340200/1‑71 (MQ=255) gAATCTGCACCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACg > 1:1657118/1‑71 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttc > 1:324872/1‑48 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACcttc > 1:4306377/1‑48 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAAc < 1:5082804/69‑1 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGt > 1:1580229/1‑71 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGt > 1:722958/1‑71 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGt > 1:5678957/1‑71 (MQ=255) aaTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGt < 1:2954064/71‑1 (MQ=255) aTCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGtt < 1:5282395/71‑1 (MQ=255) | GCCAAAGAAGGCCATCGGTACGAATACCGCCGACAGTACCATCGCGATACCGACCAGAGCGCCCTGAATCTGCCCCATCGACTTACGGGTAGCTTCTTTTGGCGGCAAACCTTCTTCCGCCATAACACGCTCAACGTT > minE/311493‑311630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |