Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,094,822 A→G 8.9% I10I (ATT→ATC tdcG ← L‑serine dehydratase 3

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,094,8220AG 8.9% 376.5 / 19.1 134I10I (ATT→ATCtdcGL‑serine dehydratase 3
Reads supporting (aligned to +/- strand):  ref base A (53/69);  new base G (8/4);  total (61/73)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 135 total aligned reads displayed.

TCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAA  >  minE/2094760‑2094892
                                                              |                                                                      
tCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGa                                                                 >  1:3134508/1‑70 (MQ=255)
  aaTAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa                                                              <  1:4592910/71‑1 (MQ=255)
      aaaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATc                                                          <  1:5076281/71‑1 (MQ=255)
       aaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg                                                         <  1:1765634/71‑1 (MQ=255)
       aaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg                                                         <  1:3942990/71‑1 (MQ=255)
        aCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGa                                                        <  1:218863/71‑1 (MQ=255)
           ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     >  1:3337653/1‑71 (MQ=255)
           ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     >  1:2724242/1‑71 (MQ=255)
           ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     >  1:5202255/1‑71 (MQ=255)
            tttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAAt                                                                      >  1:3459377/1‑53 (MQ=255)
            tttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAAt                                                                      >  1:5331385/1‑53 (MQ=255)
                cGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACt                                                 <  1:4448373/70‑1 (MQ=255)
                 ggCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTaa                                               <  1:3967628/71‑1 (MQ=255)
                   ctttcattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAAta                                                     >  1:4994076/3‑62 (MQ=255)
                   ctttcattGGTCCAACGGTATGAGAACTGGAAGGGCAAATGCCGATTTTGAAAata                                                            >  1:2413606/3‑56 (MQ=255)
                    tttcattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAAta                                                     >  1:2722853/2‑61 (MQ=255)
                    attcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCa                                            >  1:1953523/1‑71 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaa                                                                <  1:3304151/50‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg                                                         <  1:1480598/57‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg                                                         <  1:4444206/57‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     <  1:4680636/61‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     <  1:2780132/61‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     <  1:4988320/61‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     <  1:4967718/61‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     <  1:4937353/61‑1 (MQ=255)
                     ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCat                                           <  1:1549673/71‑1 (MQ=255)
                      tcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATc                                                          <  1:201504/55‑1 (MQ=255)
                      tcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCata                                          >  1:4600281/1‑71 (MQ=255)
                      tcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCata                                          >  1:185753/1‑71 (MQ=255)
                       cattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGaaaa                                                              >  1:2021963/1‑50 (MQ=255)
                        attGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCatat                                         <  1:3673656/70‑1 (MQ=255)
                        attGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATc                                        <  1:2866449/71‑1 (MQ=255)
                         ttGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATc                                        <  1:4339447/70‑1 (MQ=255)
                          tGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAATACGCTAATCatagga                                       <  1:3516884/70‑4 (MQ=255)
                           ggCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAatat                                                           >  1:335444/1‑49 (MQ=255)
                           ggCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat                                                             >  1:3137799/1‑47 (MQ=255)
                           ggCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCca                                      <  1:1537781/70‑1 (MQ=255)
                               ccACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACAccc                                 >  1:5661671/1‑71 (MQ=255)
                               ccACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACAcc                                  >  1:4446386/1‑70 (MQ=255)
                                cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTg                                                                  <  1:4752548/37‑1 (MQ=255)
                                cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTg                                                                  <  1:5688508/37‑1 (MQ=255)
                                cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat                                                             >  1:5219107/1‑42 (MQ=255)
                                cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCt                                >  1:3152767/1‑71 (MQ=255)
                                 aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa                                                              >  1:770185/1‑40 (MQ=255)
                                 aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa                                                              >  1:745586/1‑40 (MQ=255)
                                 aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg                                                     >  1:4065286/1‑49 (MQ=255)
                                 aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCtg                               >  1:5020014/1‑70 (MQ=255)
                                  cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaa                                                                <  1:4805529/37‑1 (MQ=255)
                                  cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaa                                                                <  1:4042463/37‑1 (MQ=255)
                                  cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCg                              <  1:2278327/71‑1 (MQ=255)
                                  cGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAat                                                             <  1:5658574/40‑1 (MQ=16)
                                   ggTATGAGAACTGGAAGGGCCAATGCCGATTTTGaaaa                                                              >  1:3166002/1‑38 (MQ=16)
                                    gTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa                                                              >  1:4926920/1‑37 (MQ=255)
                                    gTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa                                                              >  1:5399223/1‑37 (MQ=255)
                                    gTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa                                                              >  1:663403/1‑37 (MQ=255)
                                    gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat                                                             >  1:1046720/1‑38 (MQ=255)
                                    gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa                            <  1:4223379/71‑1 (MQ=255)
                                     tATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAt                           >  1:2882038/1‑71 (MQ=255)
                                       tGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa                                                       >  1:834518/1‑41 (MQ=255)
                                        gCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg                                                         <  1:1744917/38‑1 (MQ=255)
                                         cGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg                                                         <  1:3115342/37‑1 (MQ=255)
                                         cGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGcc                       >  1:2376258/1‑71 (MQ=255)
                                          gAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa                                                       >  1:927602/1‑38 (MQ=255)
                                           aaCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCg                              <  1:4262326/62‑1 (MQ=255)
                                           aaCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGt                     >  1:2702154/1‑71 (MQ=255)
                                           aaCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGt                     >  1:468979/1‑71 (MQ=255)
                                            aCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGt                     <  1:1502071/70‑1 (MQ=255)
                                             cTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAAt                                              <  1:1651229/44‑1 (MQ=255)
                                             cTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt                    >  1:4034579/1‑70 (MQ=255)
                                              tGGAGGGACCAATCCCAATTTTGAAAATATCGAATGc                                                    <  1:1646663/37‑1 (MQ=255)
                                              tGGAGGGACCAATCCCAATTTTGAAAATATCGAATGc                                                    <  1:1482948/37‑1 (MQ=255)
                                                gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATc                                             >  1:1207226/1‑42 (MQ=255)
                                                gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAg                 <  1:180410/70‑1 (MQ=255)
                                                gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAg                 <  1:4689144/70‑1 (MQ=255)
                                                 aGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTg               <  1:5473185/71‑1 (MQ=255)
                                                  gggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa                            >  1:349613/1‑57 (MQ=255)
                                                  gggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTg               >  1:961928/1‑70 (MQ=255)
                                                  gggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGa              <  1:2657072/71‑1 (MQ=255)
                                                   ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt                    <  1:2616037/64‑1 (MQ=255)
                                                   ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt                    <  1:598908/64‑1 (MQ=255)
                                                   ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGaa             <  1:2248919/71‑1 (MQ=255)
                                                    gACCAATCCCAATTTTGAAAATATCGAATGCACTaa                                               >  1:100761/1‑36 (MQ=255)
                                                    gACCAATCCCAATTTTGAAAATATCGAATGCACTAAt                                              >  1:421468/1‑37 (MQ=255)
                                                       cAATCCCAATTTTGAAAATATCGAATGCACTAATCata                                          >  1:1683450/1‑38 (MQ=255)
                                                       cAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTc                   >  1:1676745/1‑61 (MQ=255)
                                                          tCCCAATTTTGAAAATATCGAATGCACTAATCatat                                         <  1:2456623/36‑1 (MQ=255)
                                                          tCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa                            >  1:5154677/1‑49 (MQ=255)
                                                          tCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa                            >  1:5276957/1‑49 (MQ=255)
                                                          tCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGc      >  1:393538/1‑71 (MQ=255)
                                                           cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa                            >  1:4175596/1‑48 (MQ=255)
                                                           cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAtt                          <  1:1560363/50‑1 (MQ=255)
                                                           cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAtt                          <  1:4828909/50‑1 (MQ=255)
                                                            ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCg                              >  1:5463296/1‑45 (MQ=255)
                                                            ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt                    <  1:5513208/55‑1 (MQ=255)
                                                            ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCgg    <  1:2831372/71‑1 (MQ=255)
                                                             cAATTTTGAAAATATCGAATGCACTAATCATATCCacac                                   >  1:3621842/1‑39 (MQ=255)
                                                             cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGc                        >  1:46103/1‑50 (MQ=255)
                                                             cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCg                      >  1:5441437/1‑52 (MQ=255)
                                                             cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCg                      >  1:3661698/1‑52 (MQ=255)
                                                             cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAg            >  1:687235/1‑62 (MQ=255)
                                                              |                                                                      
TCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAA  >  minE/2094760‑2094892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: