| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,094,822 | A→G | 8.9% | I10I (ATT→ATC) | tdcG ← | L‑serine dehydratase 3 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,094,822 | 0 | A | G | 8.9% | 376.5 / 19.1 | 134 | I10I (ATT→ATC) | tdcG | L‑serine dehydratase 3 |
| Reads supporting (aligned to +/- strand): ref base A (53/69); new base G (8/4); total (61/73) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.41e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
TCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAA > minE/2094760‑2094892 | tCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGa > 1:3134508/1‑70 (MQ=255) aaTAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa < 1:4592910/71‑1 (MQ=255) aaaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATc < 1:5076281/71‑1 (MQ=255) aaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg < 1:1765634/71‑1 (MQ=255) aaCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg < 1:3942990/71‑1 (MQ=255) aCTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGa < 1:218863/71‑1 (MQ=255) ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:3337653/1‑71 (MQ=255) ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:2724242/1‑71 (MQ=255) ttttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:5202255/1‑71 (MQ=255) tttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAAt > 1:3459377/1‑53 (MQ=255) tttCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAAt > 1:5331385/1‑53 (MQ=255) cGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACt < 1:4448373/70‑1 (MQ=255) ggCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTaa < 1:3967628/71‑1 (MQ=255) ctttcattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAAta > 1:4994076/3‑62 (MQ=255) ctttcattGGTCCAACGGTATGAGAACTGGAAGGGCAAATGCCGATTTTGAAAata > 1:2413606/3‑56 (MQ=255) tttcattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAAta > 1:2722853/2‑61 (MQ=255) attcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCa > 1:1953523/1‑71 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaa < 1:3304151/50‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg < 1:1480598/57‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg < 1:4444206/57‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg < 1:4680636/61‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg < 1:2780132/61‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg < 1:4988320/61‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg < 1:4967718/61‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg < 1:4937353/61‑1 (MQ=255) ttcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCat < 1:1549673/71‑1 (MQ=255) tcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATc < 1:201504/55‑1 (MQ=255) tcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCata > 1:4600281/1‑71 (MQ=255) tcattGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCata > 1:185753/1‑71 (MQ=255) cattGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGaaaa > 1:2021963/1‑50 (MQ=255) attGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCatat < 1:3673656/70‑1 (MQ=255) attGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATc < 1:2866449/71‑1 (MQ=255) ttGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATc < 1:4339447/70‑1 (MQ=255) tGGTCCAACGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAATATCGAATACGCTAATCatagga < 1:3516884/70‑4 (MQ=255) ggCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAatat > 1:335444/1‑49 (MQ=255) ggCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat > 1:3137799/1‑47 (MQ=255) ggCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCca < 1:1537781/70‑1 (MQ=255) ccACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACAccc > 1:5661671/1‑71 (MQ=255) ccACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACAcc > 1:4446386/1‑70 (MQ=255) cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTg < 1:4752548/37‑1 (MQ=255) cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTg < 1:5688508/37‑1 (MQ=255) cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat > 1:5219107/1‑42 (MQ=255) cACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCt > 1:3152767/1‑71 (MQ=255) aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa > 1:770185/1‑40 (MQ=255) aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa > 1:745586/1‑40 (MQ=255) aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATg > 1:4065286/1‑49 (MQ=255) aCGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCtg > 1:5020014/1‑70 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaa < 1:4805529/37‑1 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGaa < 1:4042463/37‑1 (MQ=255) cGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCg < 1:2278327/71‑1 (MQ=255) cGGTATGAGAACTGGAAGGGCCAATGCCGATTTTGAAAat < 1:5658574/40‑1 (MQ=16) ggTATGAGAACTGGAAGGGCCAATGCCGATTTTGaaaa > 1:3166002/1‑38 (MQ=16) gTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa > 1:4926920/1‑37 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa > 1:5399223/1‑37 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGaaaa > 1:663403/1‑37 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAat > 1:1046720/1‑38 (MQ=255) gTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa < 1:4223379/71‑1 (MQ=255) tATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAt > 1:2882038/1‑71 (MQ=255) tGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:834518/1‑41 (MQ=255) gCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg < 1:1744917/38‑1 (MQ=255) cGAACTGGAGGGACCAATCCCAATTTTGAAAATATCg < 1:3115342/37‑1 (MQ=255) cGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGcc > 1:2376258/1‑71 (MQ=255) gAACTGGAGGGACCAATCCCAATTTTGAAAATATCGaa > 1:927602/1‑38 (MQ=255) aaCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCg < 1:4262326/62‑1 (MQ=255) aaCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGt > 1:2702154/1‑71 (MQ=255) aaCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGt > 1:468979/1‑71 (MQ=255) aCTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGt < 1:1502071/70‑1 (MQ=255) cTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAAt < 1:1651229/44‑1 (MQ=255) cTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt > 1:4034579/1‑70 (MQ=255) tGGAGGGACCAATCCCAATTTTGAAAATATCGAATGc < 1:1646663/37‑1 (MQ=255) tGGAGGGACCAATCCCAATTTTGAAAATATCGAATGc < 1:1482948/37‑1 (MQ=255) gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATc > 1:1207226/1‑42 (MQ=255) gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAg < 1:180410/70‑1 (MQ=255) gAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAg < 1:4689144/70‑1 (MQ=255) aGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTg < 1:5473185/71‑1 (MQ=255) gggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa > 1:349613/1‑57 (MQ=255) gggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTg > 1:961928/1‑70 (MQ=255) gggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGa < 1:2657072/71‑1 (MQ=255) ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt < 1:2616037/64‑1 (MQ=255) ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt < 1:598908/64‑1 (MQ=255) ggACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGaa < 1:2248919/71‑1 (MQ=255) gACCAATCCCAATTTTGAAAATATCGAATGCACTaa > 1:100761/1‑36 (MQ=255) gACCAATCCCAATTTTGAAAATATCGAATGCACTAAt > 1:421468/1‑37 (MQ=255) cAATCCCAATTTTGAAAATATCGAATGCACTAATCata > 1:1683450/1‑38 (MQ=255) cAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTc > 1:1676745/1‑61 (MQ=255) tCCCAATTTTGAAAATATCGAATGCACTAATCatat < 1:2456623/36‑1 (MQ=255) tCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa > 1:5154677/1‑49 (MQ=255) tCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa > 1:5276957/1‑49 (MQ=255) tCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGc > 1:393538/1‑71 (MQ=255) cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGa > 1:4175596/1‑48 (MQ=255) cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAtt < 1:1560363/50‑1 (MQ=255) cccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGAtt < 1:4828909/50‑1 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCg > 1:5463296/1‑45 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGtt < 1:5513208/55‑1 (MQ=255) ccAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCgg < 1:2831372/71‑1 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATCCacac > 1:3621842/1‑39 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGc > 1:46103/1‑50 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCg > 1:5441437/1‑52 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCg > 1:3661698/1‑52 (MQ=255) cAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAg > 1:687235/1‑62 (MQ=255) | TCAATAAAACTTTTTCCGGCATTCATTGGCCCCACGGTATGCGAACTGGAGGGACCAATCCCAATTTTGAAAATATCGAATGCACTAATCATATCCACACCCTCGGATTGCCGTTCAGTGAAGTGGAGCGGAA > minE/2094760‑2094892 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |