| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,096,591 | T→C | 8.3% | E116E (GAA→GAG) | garK ← | glycerate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,096,591 | 0 | T | C | 8.3% | 163.9 / 4.9 | 60 | E116E (GAA→GAG) | garK | glycerate kinase I |
| Reads supporting (aligned to +/- strand): ref base T (26/29); new base C (2/3); total (28/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.24e-01 | |||||||||||
GCGTGAAGTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTG > minE/2096522‑2096661 | gcgTGAAGTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCAttt < 1:1031675/71‑1 (MQ=255)gcgTGAAGTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCAttt > 1:643035/1‑71 (MQ=255) gTGCAGTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCACCAGCCCACTGGCCGCCGCCATTTCa < 1:1717968/71‑1 (MQ=255) aaGTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAAt > 1:4348168/1‑70 (MQ=255) aaGTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAAt > 1:5198934/1‑70 (MQ=255) gTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCa < 1:4720065/66‑1 (MQ=255) gTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCa < 1:2078999/66‑1 (MQ=255) cACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATaaa > 1:4661834/1‑66 (MQ=255) aCGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGt < 1:1169047/71‑1 (MQ=255) aGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGttt > 1:3821196/1‑70 (MQ=255) tGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc < 1:522963/71‑1 (MQ=255) tGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc < 1:4657247/71‑1 (MQ=255) ggATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc < 1:979523/70‑1 (MQ=255) ggATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc > 1:2143747/1‑70 (MQ=255) gATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc < 1:5144728/70‑1 (MQ=255) tCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATc > 1:146437/1‑71 (MQ=255) cgcgTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCa > 1:3487636/1‑50 (MQ=255) ttttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGcc > 1:2997059/1‑68 (MQ=255) tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg > 1:3010229/1‑68 (MQ=255) tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGga < 1:5280595/70‑1 (MQ=255) ttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTg > 1:1095900/1‑58 (MQ=255) ttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg > 1:2439995/1‑67 (MQ=255) tCCGCAGGTACCTGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga < 1:1582598/70‑1 (MQ=255) ccGCAGGTACCAGCTCCAGCCCACTGGCCGCCG‑CATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc > 1:787884/1‑71 (MQ=255) cGCATGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc < 1:3388872/70‑1 (MQ=255) gCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGt < 1:675312/50‑1 (MQ=255) gCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGt < 1:1759723/50‑1 (MQ=255) gCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccc > 1:4005276/1‑71 (MQ=255) tACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc < 1:602647/71‑1 (MQ=255) ccAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc < 1:814367/49‑1 (MQ=255) cAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg < 1:3979976/71‑1 (MQ=255) aGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc < 1:4865336/47‑1 (MQ=255) cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc > 1:1091799/1‑59 (MQ=255) cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc > 1:1253572/1‑58 (MQ=255) tCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa > 1:2957591/1‑63 (MQ=255) cAGCCCACTGGCGGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCa < 1:2768444/71‑1 (MQ=255) ccACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt < 1:506739/71‑1 (MQ=255) aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga < 1:755891/47‑1 (MQ=255) aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCa < 1:2198443/54‑1 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:4667717/52‑1 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:1745855/52‑1 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtctc > 1:3926480/1‑71 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCCGAGATccccc < 1:179563/52‑1 (MQ=255) tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg > 1:3593843/1‑71 (MQ=255) tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg > 1:4799295/1‑71 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa > 1:3957323/1‑52 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa > 1:4515923/1‑52 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc > 1:2219952/1‑53 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGc > 1:2411976/1‑71 (MQ=255) gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc > 1:786951/4‑71 (MQ=255) gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc > 1:697326/4‑71 (MQ=255) ctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc < 1:5535532/68‑1 (MQ=255) ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg < 1:395542/54‑1 (MQ=255) ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGccc < 1:3151299/71‑1 (MQ=255) ccgccATTTCAATAAACGCGGTTTTGCCATCGCCg < 1:4194555/35‑1 (MQ=255) ccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCa < 1:526871/69‑1 (MQ=255) cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:5388770/44‑1 (MQ=255) cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:3167975/44‑1 (MQ=255) cgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCATCg < 1:5037709/71‑1 (MQ=255) gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc > 1:605773/1‑39 (MQ=255) gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc > 1:5606833/1‑46 (MQ=255) ccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGc > 1:433689/1‑71 (MQ=255) tttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCt > 1:5273455/1‑71 (MQ=255) ttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTg > 1:5572796/1‑71 (MQ=255) | GCGTGAAGTGGTCACGAGTGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTG > minE/2096522‑2096661 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |