| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,096,609 | G→C | 6.7% | G110G (GGC→GGG) | garK ← | glycerate kinase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,096,609 | 0 | G | C | 6.7% | 223.2 / 4.7 | 74 | G110G (GGC→GGG) | garK | glycerate kinase I |
| Reads supporting (aligned to +/- strand): ref base G (37/32); new base C (2/3); total (39/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.62e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.54e-01 | |||||||||||
TGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTT > minE/2096540‑2096679 | tGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc < 1:522963/71‑1 (MQ=255)tGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc < 1:4657247/71‑1 (MQ=255) ggATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc < 1:979523/70‑1 (MQ=255) ggATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGc > 1:2143747/1‑70 (MQ=255) gATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc < 1:5144728/70‑1 (MQ=255) tCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATc > 1:146437/1‑71 (MQ=255) ttttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGcc > 1:2997059/1‑68 (MQ=255) tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg > 1:3010229/1‑68 (MQ=255) tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGga < 1:5280595/70‑1 (MQ=255) ttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTg > 1:1095900/1‑58 (MQ=255) ttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg > 1:2439995/1‑67 (MQ=255) tCCGCAGGTACCTGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga < 1:1582598/70‑1 (MQ=255) ccGCAGGTACCAGCTCCAGCCCACTGGCCGCCG‑CATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc > 1:787884/1‑71 (MQ=255) cGCATGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc < 1:3388872/70‑1 (MQ=255) gCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccc > 1:4005276/1‑71 (MQ=255) tACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc < 1:602647/71‑1 (MQ=255) ccAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc < 1:814367/49‑1 (MQ=255) cAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg < 1:3979976/71‑1 (MQ=255) aGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGcc < 1:4865336/47‑1 (MQ=255) cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc > 1:1091799/1‑59 (MQ=255) cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc > 1:1253572/1‑58 (MQ=255) tCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa > 1:2957591/1‑63 (MQ=255) cAGCCCACTGGCGGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCa < 1:2768444/71‑1 (MQ=255) ccACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt < 1:506739/71‑1 (MQ=255) aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga < 1:755891/47‑1 (MQ=255) aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCa < 1:2198443/54‑1 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:1745855/52‑1 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:4667717/52‑1 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtctc > 1:3926480/1‑71 (MQ=255) cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCCGAGATccccc < 1:179563/52‑1 (MQ=255) tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg > 1:3593843/1‑71 (MQ=255) tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg > 1:4799295/1‑71 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa > 1:4515923/1‑52 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa > 1:3957323/1‑52 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc > 1:2219952/1‑53 (MQ=255) ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGc > 1:2411976/1‑71 (MQ=255) gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc > 1:786951/4‑71 (MQ=255) gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc > 1:697326/4‑71 (MQ=255) ctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc < 1:5535532/68‑1 (MQ=255) ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg < 1:395542/54‑1 (MQ=255) ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGccc < 1:3151299/71‑1 (MQ=255) ccgccATTTCAATAAACGCGGTTTTGCCATCGCCg < 1:4194555/35‑1 (MQ=255) ccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCa < 1:526871/69‑1 (MQ=255) cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:3167975/44‑1 (MQ=255) cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc < 1:5388770/44‑1 (MQ=255) cgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCATCg < 1:5037709/71‑1 (MQ=255) gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc > 1:605773/1‑39 (MQ=255) gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc > 1:5606833/1‑46 (MQ=255) ccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGc > 1:433689/1‑71 (MQ=255) tttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCt > 1:5273455/1‑71 (MQ=255) ttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTg > 1:5572796/1‑71 (MQ=255) tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCAtt < 1:5260805/47‑1 (MQ=255) tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct > 1:5192297/1‑55 (MQ=255) tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtc > 1:3790962/1‑54 (MQ=255) aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc > 1:3670230/1‑71 (MQ=255) aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc > 1:3663530/1‑71 (MQ=255) tAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg > 1:3800223/1‑58 (MQ=255) aaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCa < 1:4018471/71‑1 (MQ=255) cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt > 1:4705379/1‑43 (MQ=255) cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt > 1:4415560/1‑43 (MQ=255) gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct > 1:1380603/1‑46 (MQ=255) gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct > 1:922753/1‑46 (MQ=255) cgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCg < 1:2474521/71‑1 (MQ=255) tttGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt < 1:2655758/39‑1 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg > 1:976787/1‑47 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGcc > 1:4816747/1‑52 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa < 1:660612/69‑1 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa < 1:4347880/69‑1 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc < 1:1676490/70‑1 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc < 1:3019747/70‑1 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg > 1:3011316/1‑71 (MQ=255) ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTATCGCCCAGCGGCCCTGTAACCCAGGCGTGa < 1:5476708/69‑1 (MQ=255) ttGCCAGCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg > 1:737594/1‑71 (MQ=255) tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt > 1:1211212/1‑71 (MQ=255) tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt > 1:5274956/1‑71 (MQ=255) gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg > 1:592681/1‑69 (MQ=255) gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGtt > 1:4407448/1‑71 (MQ=255) | TGGATCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTT > minE/2096540‑2096679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |