Predicted mutation
evidence seq id position mutation freq annotation gene description
RA minE 2,096,612 A→G 7.2% D109D (GAT→GAC)  garK ← glycerate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*minE2,096,6120AG 7.2% 192.1 / 4.8 69D109D (GAT→GAC) garKglycerate kinase I
Reads supporting (aligned to +/- strand):  ref base A (38/26);  new base G (2/3);  total (40/29)
Fisher's exact test for biased strand distribution p-value = 6.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

TCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTT  >  minE/2096544‑2096679
                                                                    |                                                                   
tCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATc                                                                   >  1:146437/1‑71 (MQ=255)
      ttttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGcc                                                                >  1:2997059/1‑68 (MQ=255)
       tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg                                                               >  1:3010229/1‑68 (MQ=255)
       tttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGga                                                             <  1:5280595/70‑1 (MQ=255)
        ttCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCg                                                               >  1:2439995/1‑67 (MQ=255)
         tCCGCAGGTACCTGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga                                                           <  1:1582598/70‑1 (MQ=255)
          ccGCAGGTACCAGCTCCAGCCCACTGGCCGCCG‑CATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc                                                        >  1:787884/1‑71 (MQ=255)
           cGCATGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc                                                         <  1:3388872/70‑1 (MQ=255)
            gCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccc                                                       >  1:4005276/1‑71 (MQ=255)
                 tACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                  <  1:602647/71‑1 (MQ=255)
                    cAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg                                               <  1:3979976/71‑1 (MQ=255)
                       cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATcc                                                        >  1:1091799/1‑59 (MQ=255)
                       cTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc                                                         >  1:1253572/1‑58 (MQ=255)
                        tCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa                                                   >  1:2957591/1‑63 (MQ=255)
                          cAGCCCACTGGCGGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCa                                         <  1:2768444/71‑1 (MQ=255)
                              ccACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt                                     <  1:506739/71‑1 (MQ=255)
                                aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGgaga                                                           <  1:755891/47‑1 (MQ=255)
                                aCTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCa                                                    <  1:2198443/54‑1 (MQ=255)
                                 cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:1745855/52‑1 (MQ=255)
                                 cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:4667717/52‑1 (MQ=255)
                                 cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtctc                                  >  1:3926480/1‑71 (MQ=255)
                                 cTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCCGAGATccccc                                                     <  1:179563/52‑1 (MQ=255)
                                  tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg                                 >  1:3593843/1‑71 (MQ=255)
                                  tGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCg                                 >  1:4799295/1‑71 (MQ=255)
                                   ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa                                                   >  1:4515923/1‑52 (MQ=255)
                                   ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCaa                                                   >  1:3957323/1‑52 (MQ=255)
                                   ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                  >  1:2219952/1‑53 (MQ=255)
                                   ggCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGc                                >  1:2411976/1‑71 (MQ=255)
                                    gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                               >  1:697326/4‑71 (MQ=255)
                                    gctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                               >  1:786951/4‑71 (MQ=255)
                                     ctgccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGcc                               <  1:5535532/68‑1 (MQ=255)
                                     ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTgg                                               <  1:395542/54‑1 (MQ=255)
                                     ccgccgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGccc                              <  1:3151299/71‑1 (MQ=255)
                                        ccgccATTTCAATAAACGCGGTTTTGCCATCGCCg                                                               <  1:4194555/35‑1 (MQ=255)
                                        ccgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCa                             <  1:526871/69‑1 (MQ=255)
                                         cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:5388770/44‑1 (MQ=255)
                                         cgccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATccccc                                                     <  1:3167975/44‑1 (MQ=255)
                                         cgccATCTCAATCACCGCCGTTTTCCCGTCGCCGGTAAGGCCATAAAAAGCATTCACTTTTTCGCCCATCg                          <  1:5037709/71‑1 (MQ=255)
                                          gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATc                                                         >  1:605773/1‑39 (MQ=255)
                                          gccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAAc                                                  >  1:5606833/1‑46 (MQ=255)
                                           ccATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGc                        >  1:433689/1‑71 (MQ=255)
                                              tttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCt                     >  1:5273455/1‑71 (MQ=255)
                                               ttCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTg                    >  1:5572796/1‑71 (MQ=255)
                                                tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCAtt                                           <  1:5260805/47‑1 (MQ=255)
                                                tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                   >  1:5192297/1‑55 (MQ=255)
                                                tCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtc                                    >  1:3790962/1‑54 (MQ=255)
                                                   aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc                >  1:3670230/1‑71 (MQ=255)
                                                   aTAAACGCGGTTTTGACATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAAc                >  1:3663530/1‑71 (MQ=255)
                                                    tAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg                            >  1:3800223/1‑58 (MQ=255)
                                                      aaCGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCa             <  1:4018471/71‑1 (MQ=255)
                                                        cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt                                       >  1:4705379/1‑43 (MQ=255)
                                                        cgcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACt                                       >  1:4415560/1‑43 (MQ=255)
                                                         gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                   >  1:922753/1‑46 (MQ=255)
                                                         gcgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTtct                                   >  1:1380603/1‑46 (MQ=255)
                                                          cgGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCg         <  1:2474521/71‑1 (MQ=255)
                                                              tttGCCATCGCCGGAGATCCCCCAACTGGCATTCACttt                                     <  1:2655758/39‑1 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAg                            >  1:976787/1‑47 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGcc                       >  1:4816747/1‑52 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa      <  1:660612/69‑1 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGa      <  1:4347880/69‑1 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc     <  1:3019747/70‑1 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGAc     <  1:1676490/70‑1 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg    >  1:3011316/1‑71 (MQ=255)
                                                               ttGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTATCGCCCAGCGGCCCTGTAACCCAGGCGTGa      <  1:5476708/69‑1 (MQ=255)
                                                               ttGCCAGCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg    >  1:737594/1‑71 (MQ=255)
                                                                tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt   >  1:5274956/1‑71 (MQ=255)
                                                                tGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGt   >  1:1211212/1‑71 (MQ=255)
                                                                 gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACg    >  1:592681/1‑69 (MQ=255)
                                                                 gCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGtt  >  1:4407448/1‑71 (MQ=255)
                                                                    |                                                                   
TCGCGTTTTTCCGCAGGTACCAGCTCCAGCCCACTGGCCGCCGCCATTTCAATAAACGCGGTTTTGCCATCGCCGGAGATCCCCCAACTGGCATTCACTTTCTCGCCCAGCGGCCCTGTAACCCAGGCGTGACGTT  >  minE/2096544‑2096679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑