| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,104,153 | T→C | 16.0% | N51N (AAT→AAC) | kbaZ → | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,104,153 | 0 | T | C | 16.0% | 298.6 / 43.8 | 131 | N51N (AAT→AAC) | kbaZ | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base T (47/63); new base C (16/5); total (63/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.70e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01 | |||||||||||
Only 100 of 133 total aligned reads displayed.
GTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTC > minE/2104085‑2104217 | gTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATc < 1:4838487/70‑1 (MQ=255) gAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAg < 1:4578151/70‑1 (MQ=255) gAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGa > 1:1036656/1‑71 (MQ=255) aaGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc < 1:2397512/71‑1 (MQ=255) cTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATc < 1:5275941/60‑1 (MQ=255) cTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATc < 1:4732550/60‑1 (MQ=255) cTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:5440345/71‑1 (MQ=255) cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc < 1:1891971/71‑1 (MQ=255) cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:4476016/1‑71 (MQ=255) aTCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcgg < 1:5047803/71‑1 (MQ=255) tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc < 1:1224130/71‑1 (MQ=255) tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc > 1:3645520/1‑71 (MQ=255) tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc < 1:183417/71‑1 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc > 1:1308532/1‑60 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc > 1:4230862/1‑60 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcgg > 1:3361451/1‑71 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg < 1:2947930/70‑1 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg < 1:2347729/70‑1 (MQ=255) gCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:390094/71‑1 (MQ=255) gCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:948892/71‑1 (MQ=255) tACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1221334/1‑71 (MQ=255) cGCCAGTGCAAACCAAACGCCGCTACTGATTGAAGCAACCTCCAATCAGGTAGAc < 1:2169827/55‑1 (MQ=255) ccAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAg > 1:5311209/1‑51 (MQ=255) aGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCgg < 1:4261789/71‑1 (MQ=255) tGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATc < 1:217903/41‑1 (MQ=255) tGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATc < 1:740352/41‑1 (MQ=255) tGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATc < 1:3107623/41‑1 (MQ=255) tGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGaa < 1:2162299/71‑1 (MQ=255) caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTa > 1:2105455/1‑44 (MQ=255) caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTa > 1:3983490/1‑44 (MQ=255) caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTa > 1:874942/1‑44 (MQ=255) aaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa < 1:961907/71‑1 (MQ=255) aaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAg < 1:447841/43‑1 (MQ=255) aaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1162522/1‑59 (MQ=255) accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcg > 1:268734/1‑51 (MQ=255) accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcg > 1:3833679/1‑71 (MQ=255) caaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcc > 1:4833883/1‑71 (MQ=255) acGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata > 1:5337673/1‑55 (MQ=255) cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc > 1:2849938/1‑71 (MQ=255) cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc > 1:2964781/1‑71 (MQ=255) cGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt > 1:688022/1‑71 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:4670184/71‑1 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:4663429/71‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:1508459/36‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:872318/36‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:819041/36‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:5040655/45‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:2628446/45‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata < 1:5157905/49‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta < 1:3134208/47‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAttt < 1:4517395/71‑1 (MQ=255) tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTta < 1:5381246/44‑1 (MQ=255) tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActtt > 1:1452742/3‑71 (MQ=255) tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata < 1:4972172/48‑1 (MQ=255) tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCg < 1:1515090/51‑1 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg > 1:3768371/1‑41 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:5181723/1‑37 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:4137225/1‑37 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata > 1:3605181/1‑47 (MQ=255) cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata > 1:2236531/1‑46 (MQ=255) cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata > 1:4198216/1‑46 (MQ=255) cTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcg > 1:4115025/1‑71 (MQ=255) tGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActt < 1:4642488/67‑1 (MQ=255) gATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgcg > 1:1766241/1‑71 (MQ=255) aTTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcg > 1:3244603/1‑70 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATAcc < 1:4982771/45‑1 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:4570683/50‑1 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:1889668/50‑1 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2350933/1‑69 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2560392/1‑69 (MQ=255) ttGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:2531918/49‑1 (MQ=255) ttGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGc > 1:5301465/1‑71 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCgg > 1:2220899/1‑45 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:2493561/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:3330137/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:5112299/1‑68 (MQ=255) tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtat < 1:4668275/40‑1 (MQ=255) aGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGTAATGACACCGGCAGACTTTCGCGAAtttg < 1:1525802/71‑1 (MQ=255) aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1977596/1‑36 (MQ=255) aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1862434/1‑36 (MQ=255) aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1416292/1‑36 (MQ=255) cAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGaa > 1:2724506/1‑42 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGa > 1:3224518/1‑44 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt < 1:5011078/71‑1 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt < 1:4287668/71‑1 (MQ=255) aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:5089189/71‑1 (MQ=255) aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:3228111/71‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgc > 1:982795/1‑46 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:4117836/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:959601/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgtttg < 1:1969733/71‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:4174387/1‑68 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:3450725/1‑68 (MQ=255) cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:5583957/65‑1 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgccc > 1:5458015/1‑45 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt > 1:5653725/1‑61 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt > 1:220938/1‑61 (MQ=255) aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa > 1:4285320/1‑36 (MQ=255) aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa > 1:3025590/1‑36 (MQ=255) tCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:1641440/59‑1 (MQ=255) | GTGCTGGAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTC > minE/2104085‑2104217 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |