| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,104,160 | G→A | 17.0% | D54N (GAC→AAC) ‡ | kbaZ → | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,104,160 | 0 | G | A | 17.0% | 279.4 / 42.5 | 123 | D54N (GAC→AAC) ‡ | kbaZ | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base G (49/53); new base A (16/5); total (65/58) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.92e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
Only 100 of 128 total aligned reads displayed.
GAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTC > minE/2104091‑2104230 | gAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGa > 1:1036656/1‑71 (MQ=255) aaGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc < 1:2397512/71‑1 (MQ=255) cTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:5440345/71‑1 (MQ=255) cTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:2181598/71‑1 (MQ=255) cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc < 1:2613918/71‑1 (MQ=255) cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:1552048/1‑71 (MQ=255) cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:4476016/1‑71 (MQ=255) cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc < 1:1891971/71‑1 (MQ=255) tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc > 1:3645520/1‑71 (MQ=255) tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc < 1:183417/71‑1 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc > 1:1308532/1‑60 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGAc > 1:4230862/1‑60 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcgg > 1:3361451/1‑71 (MQ=255) gCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:948892/71‑1 (MQ=255) gCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:390094/71‑1 (MQ=255) tACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1221334/1‑71 (MQ=255) actccAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:4658344/4‑70 (MQ=255) cGCCAGTGCAAACCAAACGCCGCTACTGATTGAAGCAACCTCCAATCAGGTAGAc < 1:2169827/55‑1 (MQ=255) ccAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAg > 1:5311209/1‑51 (MQ=255) aGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCgg < 1:4261789/71‑1 (MQ=255) tGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGaa < 1:2162299/71‑1 (MQ=255) caatccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGTTAGACCAGTTCGGCGGTTATACCGGAAt < 1:2460635/70‑1 (MQ=255) caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt > 1:1291697/1‑70 (MQ=255) aaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa < 1:961907/71‑1 (MQ=255) aaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAg < 1:447841/43‑1 (MQ=255) aaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAg < 1:833901/43‑1 (MQ=255) aaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1162522/1‑59 (MQ=255) caaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcc > 1:4833883/1‑71 (MQ=255) acGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata > 1:5337673/1‑55 (MQ=255) cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc > 1:2849938/1‑71 (MQ=255) cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc > 1:2964781/1‑71 (MQ=255) gCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCccgccg < 1:5108881/71‑1 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:4663429/71‑1 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:5237288/71‑1 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:4670184/71‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:872318/36‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:819041/36‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:2823204/36‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAg < 1:1508459/36‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:5040655/45‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:2628446/45‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata < 1:5157905/49‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta < 1:3134208/47‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAttt < 1:4517395/71‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:571206/70‑1 (MQ=255) tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTta < 1:5381246/44‑1 (MQ=255) tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActtt > 1:1452742/3‑71 (MQ=255) tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata < 1:4972172/48‑1 (MQ=255) tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCg < 1:1515090/51‑1 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg > 1:3768371/1‑41 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCAGCCGATTTTc > 1:3781488/1‑71 (MQ=255) cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata > 1:2236531/1‑46 (MQ=255) cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata > 1:4198216/1‑46 (MQ=255) cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata > 1:4144947/1‑46 (MQ=255) cTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcg > 1:4115025/1‑71 (MQ=255) tGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActt < 1:4642488/67‑1 (MQ=255) gATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgcg > 1:1766241/1‑71 (MQ=255) aTTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcg > 1:3244603/1‑70 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:1889668/50‑1 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:4570683/50‑1 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2560392/1‑69 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2350933/1‑69 (MQ=255) ttGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGc > 1:5301465/1‑71 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCgg > 1:2220899/1‑45 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:5112299/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:2493561/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:3330137/1‑68 (MQ=255) tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtat < 1:4668275/40‑1 (MQ=255) tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtat < 1:4066572/40‑1 (MQ=255) tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACTCCCGCCGATTTTCGCgg > 1:189999/1‑69 (MQ=255) gAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:2316795/1‑38 (MQ=255) aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1977596/1‑36 (MQ=255) aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1416292/1‑36 (MQ=255) cAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGaa > 1:2724506/1‑42 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGa > 1:491660/1‑44 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGa > 1:3224518/1‑44 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt < 1:5011078/71‑1 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt < 1:4287668/71‑1 (MQ=255) aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:3228111/71‑1 (MQ=255) aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:5089189/71‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgc > 1:982795/1‑46 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:4117836/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:959601/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:3450725/1‑68 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:4174387/1‑68 (MQ=255) ccTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:5441795/40‑1 (MQ=255) ccTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:5025624/40‑1 (MQ=255) cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:5583957/65‑1 (MQ=255) cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc < 1:3011683/71‑1 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt > 1:5653725/1‑61 (MQ=255) aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa > 1:3025590/1‑36 (MQ=255) aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa > 1:4285320/1‑36 (MQ=255) tCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:1641440/59‑1 (MQ=255) cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGc > 1:3287018/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgc > 1:4649050/1‑50 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:4632385/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:2577266/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:1520900/1‑71 (MQ=255) gTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:1211817/58‑1 (MQ=255) tAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGa > 1:5044543/1‑70 (MQ=255) | GAAGCTGCAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTC > minE/2104091‑2104230 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |