| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,104,168 | C→T | 20.4% | F56F (TTC→TTT) | kbaZ → | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,104,168 | 0 | C | T | 20.4% | 208.1 / 44.6 | 103 | F56F (TTC→TTT) | kbaZ | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base C (38/44); new base T (16/5); total (54/49) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.60e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 121 total aligned reads displayed.
CAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATT > minE/2104098‑2104238 | cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc < 1:1891971/71‑1 (MQ=255)cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:4476016/1‑71 (MQ=255)cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:1552048/1‑71 (MQ=255)cAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc < 1:2613918/71‑1 (MQ=255) aTCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcgg < 1:5047803/71‑1 (MQ=255) tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc < 1:1224130/71‑1 (MQ=255) tCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggc > 1:3645520/1‑71 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcgg > 1:3361451/1‑71 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg < 1:2947930/70‑1 (MQ=255) cGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg < 1:2347729/70‑1 (MQ=255) gCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:390094/71‑1 (MQ=255) actccAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:4658344/4‑70 (MQ=255) aGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCgg < 1:4261789/71‑1 (MQ=255) tGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGaa < 1:2162299/71‑1 (MQ=255) caatccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGTTAGACCAGTTCGGCGGTTATACCGGAAt < 1:2460635/70‑1 (MQ=255) caaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt > 1:1291697/1‑70 (MQ=255) aaaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa < 1:961907/71‑1 (MQ=255) aaccaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1162522/1‑59 (MQ=255) accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcg > 1:268734/1‑51 (MQ=255) accaaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcg > 1:3833679/1‑71 (MQ=255) caaacGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcc > 1:4833883/1‑71 (MQ=255) acGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata > 1:5337673/1‑55 (MQ=255) cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc > 1:2849938/1‑71 (MQ=255) cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc > 1:2964781/1‑71 (MQ=255) gCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCccgccg < 1:5108881/71‑1 (MQ=255) cGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt > 1:688022/1‑71 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:4663429/71‑1 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:5237288/71‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGt < 1:5040655/45‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata < 1:5157905/49‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta < 1:3134208/47‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAttt < 1:4517395/71‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:571206/70‑1 (MQ=255) tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTta < 1:5381246/44‑1 (MQ=255) tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActtt > 1:1452742/3‑71 (MQ=255) tACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtata < 1:4972172/48‑1 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTcggcg > 1:3768371/1‑41 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:4137225/1‑37 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTc > 1:5181723/1‑37 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCAGCCGATTTTc > 1:3781488/1‑71 (MQ=255) cTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTtata > 1:4144947/1‑46 (MQ=255) cTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcg > 1:4115025/1‑71 (MQ=255) tGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActt < 1:4642488/67‑1 (MQ=255) gATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgcg > 1:1766241/1‑71 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATAcc < 1:4982771/45‑1 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:4570683/50‑1 (MQ=255) aTTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:1889668/50‑1 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2560392/1‑69 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2350933/1‑69 (MQ=255) ttGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:2531918/49‑1 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCgg > 1:2220899/1‑45 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:3330137/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:4235724/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:5112299/1‑68 (MQ=255) tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTtat < 1:4668275/40‑1 (MQ=255) tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACTCCCGCCGATTTTCGCgg > 1:189999/1‑69 (MQ=255) gAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:2316795/1‑38 (MQ=255) aGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGTAATGACACCGGCAGACTTTCGCGAAtttg < 1:1525802/71‑1 (MQ=255) aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1416292/1‑36 (MQ=255) aGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTta > 1:1862434/1‑36 (MQ=255) cAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGaa > 1:1464212/1‑42 (MQ=255) cAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGaa > 1:2724506/1‑42 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGa > 1:491660/1‑44 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGa > 1:3224518/1‑44 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt < 1:4287668/71‑1 (MQ=255) aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:3228111/71‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgc > 1:982795/1‑46 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:959601/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:4117836/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgtttg < 1:1969733/71‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:3450725/1‑68 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:4174387/1‑68 (MQ=255) ccTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:5025624/40‑1 (MQ=255) ccTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAAt < 1:5441795/40‑1 (MQ=255) cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:5583957/65‑1 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgccc > 1:5458015/1‑45 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt > 1:5653725/1‑61 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt > 1:220938/1‑61 (MQ=255) aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa > 1:4285320/1‑36 (MQ=255) aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa > 1:3025590/1‑36 (MQ=255) aTCAGGTAGACCAGTTCGGCGGTTATACCGGAATGa > 1:953726/1‑36 (MQ=255) tCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:1641440/59‑1 (MQ=255) tCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:5314207/59‑1 (MQ=255) cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTttgtttgt > 1:4656143/1‑63 (MQ=255) cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGc > 1:3287018/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgc > 1:4649050/1‑50 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:2577266/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:1520900/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:4632385/1‑71 (MQ=255) gTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:1211817/58‑1 (MQ=255) tAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGa > 1:5044543/1‑70 (MQ=255) gACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTc > 1:4728619/1‑71 (MQ=255) aCCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGc < 1:5684670/47‑1 (MQ=255) aGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTg < 1:1085077/71‑1 (MQ=255) gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:3600970/50‑1 (MQ=255) gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:1050824/50‑1 (MQ=255) ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:2550838/49‑1 (MQ=255) cggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:1064807/44‑1 (MQ=255) cggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCt > 1:2059892/1‑54 (MQ=255) cggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAtt > 1:24378/1‑71 (MQ=255) | CAATCCGCTACGCCAGTGCAAACCAAACGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATT > minE/2104098‑2104238 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |