| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | minE | 2,104,192 | C→G | 12.7% | P64P (CCC→CCG) | kbaZ → | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | minE | 2,104,192 | 0 | C | G | 12.7% | 243.7 / 21.4 | 95 | P64P (CCC→CCG) | kbaZ | tagatose 6‑phosphate aldolase 1, kbaZ subunit |
| Reads supporting (aligned to +/- strand): ref base C (36/46); new base G (8/4); total (45/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
Only 100 of 105 total aligned reads displayed.
CGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTG > minE/2104125‑2104261 | cGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgcccgcc > 1:2964781/1‑71 (MQ=255) gCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCccgccg < 1:5108881/71‑1 (MQ=255) cGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt > 1:688022/1‑71 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:5237288/71‑1 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:4670184/71‑1 (MQ=255) gTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:4663429/71‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAttt < 1:4517395/71‑1 (MQ=255) ttACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAtt < 1:571206/70‑1 (MQ=255) tgcTGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActtt > 1:1452742/3‑71 (MQ=255) aCTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCAGCCGATTTTc > 1:3781488/1‑71 (MQ=255) cTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcg > 1:4115025/1‑71 (MQ=255) tGATTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGActt < 1:4642488/67‑1 (MQ=255) aTTGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcg > 1:3244603/1‑70 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2350933/1‑69 (MQ=255) ttGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaa > 1:2560392/1‑69 (MQ=255) ttGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGc > 1:5301465/1‑71 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:4235724/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:3330137/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:5112299/1‑68 (MQ=255) tGAAGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTcgcgaat > 1:2493561/1‑68 (MQ=255) tGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACTCCCGCCGATTTTCGCgg > 1:189999/1‑69 (MQ=255) aGCAACGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGTAATGACACCGGCAGACTTTCGCGAAtttg < 1:1525802/71‑1 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt < 1:4287668/71‑1 (MQ=255) aaCGTCAAACCAGGTCAATCAATTTGGCGGTTATACCGGAATGACACCGGCAGACTTTCGCGAAtttgttt < 1:5011078/71‑1 (MQ=255) aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:5089189/71‑1 (MQ=255) aaCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:3228111/71‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:4117836/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGAt < 1:959601/54‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgtttg < 1:1969733/71‑1 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:4174387/1‑68 (MQ=255) aCCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:3450725/1‑68 (MQ=255) ccTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTttgtttgt < 1:4330402/71‑1 (MQ=255) cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:5583957/65‑1 (MQ=255) cTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc < 1:3011683/71‑1 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGAcgccc > 1:5458015/1‑45 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt > 1:5653725/1‑61 (MQ=255) tCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCt > 1:220938/1‑61 (MQ=255) tCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:5314207/59‑1 (MQ=255) tCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:1641440/59‑1 (MQ=255) cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTttgtttgt > 1:4656143/1‑63 (MQ=255) cAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGc > 1:3287018/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTcgc > 1:4649050/1‑50 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:2577266/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:4632385/1‑71 (MQ=255) aGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGcc > 1:1520900/1‑71 (MQ=255) gTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:1211817/58‑1 (MQ=255) gACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTc > 1:4728619/1‑71 (MQ=255) aCCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGc < 1:4646609/47‑1 (MQ=255) aCCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGc < 1:5684670/47‑1 (MQ=255) aGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTg < 1:1085077/71‑1 (MQ=255) gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:1050824/50‑1 (MQ=255) gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:3600970/50‑1 (MQ=255) gTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCTCCGACTCGTTg > 1:5497384/1‑70 (MQ=255) ttCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt < 1:2550838/49‑1 (MQ=255) cggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCtttg < 1:1064807/44‑1 (MQ=255) cggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCt > 1:2059892/1‑54 (MQ=255) cggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAtt > 1:24378/1‑71 (MQ=255) ggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAttt < 1:3279030/71‑1 (MQ=255) ggcggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAAtt < 1:1397652/70‑1 (MQ=255) cggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc < 1:1322977/47‑1 (MQ=255) cggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc < 1:3511699/47‑1 (MQ=255) cggTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc < 1:654665/47‑1 (MQ=255) gTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCg > 1:1562606/1‑71 (MQ=255) ttATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCt > 1:1570889/1‑48 (MQ=255) ttATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGc < 1:110540/71‑1 (MQ=255) ttATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGc < 1:1796869/71‑1 (MQ=255) ttATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGc < 1:4609439/71‑1 (MQ=255) ttATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGc < 1:1803659/71‑1 (MQ=255) tataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCg > 1:1695852/1‑69 (MQ=255) tataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCg > 1:2805223/1‑69 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt > 1:875173/1‑39 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt > 1:217082/1‑39 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCtttgttt > 1:3887035/1‑39 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCtttgtt > 1:140867/1‑38 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:898032/1‑37 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:4929813/1‑37 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCtttgt > 1:1132465/1‑37 (MQ=255) ataCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAg > 1:3104457/1‑71 (MQ=255) taCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAg < 1:4967494/70‑1 (MQ=255) aCCGGAATGACGCCCGCCGATTTTCGCGGCtttgt < 1:1490198/35‑1 (MQ=255) aCCGGAATGACGCCCGCCGATTTTCGCGGCtttgt < 1:164171/35‑1 (MQ=255) aCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc < 1:4168486/40‑1 (MQ=255) aCCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTc < 1:4887608/40‑1 (MQ=255) ccGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTccc < 1:2348319/64‑1 (MQ=255) ccGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGAt > 1:2578364/1‑71 (MQ=255) cGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGa > 1:3034888/1‑48 (MQ=255) gAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGc < 1:1010246/63‑1 (MQ=255) aTGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGt > 1:5342419/1‑70 (MQ=255) tGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTg < 1:614634/50‑1 (MQ=255) tGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAg < 1:4775459/62‑1 (MQ=255) tGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAg < 1:4053138/62‑1 (MQ=255) tGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAg < 1:2545375/62‑1 (MQ=255) aCGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGa < 1:2370794/70‑1 (MQ=255) aCGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGa < 1:2223626/70‑1 (MQ=255) aCGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGAt < 1:1293599/71‑1 (MQ=255) gcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTc < 1:3129834/71‑1 (MQ=255) gcccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTc < 1:3123312/71‑1 (MQ=255) cccgccGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCt > 1:2485545/1‑71 (MQ=255) ccgccgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTg > 1:3719077/1‑71 (MQ=255) ccgccgATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTg > 1:949989/1‑71 (MQ=255) | CGCCGTTACTGATTGAAGCAACCTCCAATCAGGTAGACCAGTTCGGCGGTTATACCGGAATGACGCCCGCCGATTTTCGCGGCTTTGTTTGTCAGCTCGCCGACTCGTTGAATTTCCCGCAGGATGCGTTGATTCTG > minE/2104125‑2104261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |